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pCAS-Guide vector (with Cas9 expression) for genomic target sequence cloning (10 µg), GE100008 included.
| Function | All-in-One CRISPR/Cas9 vectors |
GE100002 is the updated version of GE100001.
Vectors
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Oligo annealing buffer (10X)
| Function | Accessories |
pLenti-Cas-Guide vector (with Cas9 expression) for genomic target sequence cloning (10 µg), GE100008 included.
| Function | All-in-One CRISPR/Cas9 vectors |
GE100010 is the updated version of GE100009.
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
| Function | All-in-One CRISPR/Cas9 vectors |
CF3, the forward sequencing primer to sequence target sequence cloned in CRISPR vectors.
| Function | Accessories |
pGuide-EF1a-GFP, gRNA only vector with EF1a driven GFP
| Function | gRNA only vectors |
Vector kit for targeted transgene insertion into AAVS1 locus (EF1a, puro), including GE100023, GE100046 and GE100003
| Function | CRISPR/AAVS1 kit |
pLenti-Guide-Puro, gRNA cloning vector in lenti vector with puromycin as mammalian selection marker (10ug)
| Function | gRNA only vectors |
PCR Primer mix to confirm AAVS1 integration, amplifying the left integration junction, 2 nmols
Cas9 expression vector in AAVS1 donor vector, puromycin selection (10 ug)
| Function | Cas9 only vectors |
Vector kit for targeted transgene insertion into ROSA26 locus in mice (puro), including GE100050, GE100051 and GE100003
| Function | CRISPR/ROSA26 kit |
pLenti-EF1a-Cas9-IRES-Puro, Cas9 in lenti vector under EF1a promoter with bicistronic IRES-Puro (10 ug)
| Function | Cas9 only vectors |
pLenti-Cas9-P2A-tGFP, Cas9 in lenti vector with tGFP after P2A (10 ug)
| Function | Cas9 only vectors |
Vector kit for targeted transgene insertion into AAVS1 locus (blasticidin selection), including AAVS1 gRNA vector (cat# GE100023), AAVS1-BSD-donor (cat# GE100035) and gRNA scramble control (cat# GE100003)
| Function | CRISPR/AAVS1 kit |
Cas9 expression vector in AAVS1 donor vector, blasticidin selection (10ug)
| Function | Cas9 only vectors |
Vector kit to insert Cas9 at AAVS1 locus (puro selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-Puro donor (cat# GE100037), and a scramble gRNA control (cat# GE100003)
| Function | CRISPR/AAVS1 kit |
Vector kit for targeted transgene insertion into AAVS1 locus (EF1a, blasticidin), including GE100023, GE100048 and GE100003
| Function | CRISPR/AAVS1 kit |
Cas9 in vitro digestion buffer, 10x
| Function | Accessories |
Vector kit to insert Cas9 at AAVS1 locus (blasticidin selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-BSD donor (cat# GE100039), and a scramble gRNA control (cat# GE100003)
| Function | CRISPR/AAVS1 kit |
pLenti-EF1a-Cas-Guide, (with EF1a-Cas9) for gRNA target sequence cloning (10 µg)
| Function | All-in-One CRISPR/Cas9 vectors |
ROSA26 safe harbor gRNA vector, validated ROSA26 targeting sequence cloned in pCas-Guide vector
| Function | Rosa26 gRNA |
CRISPRa SAM vector kit containing pCas-Guide-CRISPRa (GE100055), pCRISPRa-Enhancer (GE100056) and pCas-Guide-CRISPRa-Scramble (GE100058)
| Function | CRISPRa SAM vector kit |
pCas-Guide-CRISPRa vector, CRISPRa SAM activation system vector containing dCas9-VP64 and gRNA cloning site.
| Function | All-in-one CRISPRa SAM vector |
USD 680.00
In Stock
pCRISPRa-Enhancer vector, expressing MS2-p65-HSF1, which synergistically activates gene expression with dCas9-VP64.
| Function | CRISPRa Enhancer Vector |
USD 390.00
In Stock
pCas-Guide-CRISPRa-Scramble, scramble gRNA control for CRISPRa SAM system
| Function | CRISPRa scramble control vector |
USD 480.00
2 Weeks
pCas-Guide-CRISPRi vector, all-in-one CRISPRi vector containing dCas9-KRAB-MeCP2 and gRNA cloning site.
| Function | All-in-one CRISPRi vector |
pLenti-Cas9-Myc-DDK-P2A-Puro control plasmid containing the expression cassette for Cas9-Myc-DDK fusion protein and Puromycin resistance.
| Function | Cas9 only vectors |
pCas-Guide-Nickase vector for gRNA cloning (with Cas9 D10A expression) (10 µg)
| Function | Cas9 Nickase |
pT7-Cas9-Nickase vector (Cas9 D10A) (10 µg)
| Function | Cas9 Nickase |
A scrambled sequence in pCas-Guide-EF1a-GFP vector (10 µg)
| Function | All-in-One CRISPR/Cas9 vectors |
pCas-Guide-EF1a-CD4 vector (with Cas9 and CD4 expression) for genomic target sequence cloning (10 µg)
| Function | All-in-One CRISPR/Cas9 vectors |
AAVS1 gRNA vector, validated AAVS1 targeting sequence cloned in pCas-Guide vector
| Function | All-in-One CRISPR/Cas9 vectors |
Positive control for AAVS1 insertion (RFP insertion via CRISPR)
| Function | AAVS1 donor vector |
Vector kit for targeted transgene insertion into AAVS1 locus, including GE100023, GE100024 and GE100003
| Function | CRISPR kit |
pLenti-Cas9 vector, Cas9 in lenti vector (10 ug)
| Function | Cas9 only vectors |
pLenti-Cas9-IRES-Puro, Cas9 in lenti vector with bicistronic IRES-Puro (10 ug)
| Function | Cas9 only vectors |
USD 1,000.00
2 Weeks
RFP negative transgenic cell lines (HEK293T cells containing RFP dead mutant at AAVS1 locus), can be edited to convert to RFP positive cells
USD 150.00
2 Weeks
Validated repairing donor oligos for RFP dead mutant correction in GE100041C cells
Validated sgRNA targeting RFP dead mutant in GE100041C cells
pCas-Guide-CRISPRi-Scramble, scramble gRNA control for CRISPRi SAM system
| Function | CRISPRi scramble control vector |
Catalytically inactive Cas9 (D10A/H840A) vector for gRNA cloning (10 µg)
| Function | Cas9 Nickase |
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