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ESPL1 (Myc-DDK-tagged)-Human extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

ESPL1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN417230 is the updated version of KN217230.

ESPL1 (GFP-tagged) - Human extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

ESPL1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin

ESPL1 (untagged)-Human extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1)

Vector pCMV6-XL6
Tag Tag Free
Mammalian Cell Selection None

ESPL1 CRISPRa kit - CRISPR gene activation of human extra spindle pole bodies like 1, separase

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

qSTAR qPCR primer pairs against Homo sapiens gene ESPL1

3`UTR clone of extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1) for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

Transient overexpression of ESPL1 (NM_012291) in HEK293T cells paraffin embedded controls for ICC/IHC staining

ESPL1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector

Format Retroviral plasmids
Vector pRS

Transient overexpression of ESPL1 (NM_012291) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 5 slides per pack

Transient overexpression of ESPL1 (NM_012291) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 25 slides per pack