PMS2 Mouse Monoclonal Antibody [Clone ID: OTI2G5]

CAT#: CF506658

Carrier-free (BSA/glycerol-free) PMS2 mouse monoclonal antibody, clone OTI2G5 (formerly 2G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMS2 (RC210880, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMS2. Positive lysates LY424658 (100ug) and LC424658 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and PMS2-Knockout HeLa cells (KO, Cat# LC831286) were separated by SDS-PAGE and immunoblotted with anti-PMS2 monoclonal antibody TA506658 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506658)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506658)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506658)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506658)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506658)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506658)

• Immunofluorescent staining of HeLa cells using anti-PMS2 mouse monoclonal antibody (TA506658).

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Specifications

Product Data

• Clone Name: OTI2G5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PMS2(NP_000526) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 95.6 kDa
• Gene Name: Homo sapiens PMS1 homolog 2, mismatch repair system component (PMS2), transcript variant 1, mRNA.
• Database Link:
  NP_000526
  Entrez Gene 5395 Human
  UniProt ID P54278
• Background: This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: HNPCC4; PMS2CL; PMSL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair