SLIRP Rabbit Polyclonal Antibody

CAT#: TA309873

Rabbit Polyclonal SLIRP Antibody

Product Images

Immunohistochemistry: SLIRP Antibody TA309873 - Analysis of SLIRP in mouse intestine using DAB with hematoxylin counterstain.

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  Immunocytochemistry/Immunofluorescence: SLIRP Antibody TA309873 - SLIRP antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Simple Western: SLIRP Antibody TA309873 - Simple Western lane view shows a specific band for SLIRP in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

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  Immunocytochemistry/Immunofluorescence: SLIRP Antibody TA309873 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SLIRP (TA309873) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: SLIRP Antibody TA309873 - Detection of SLIRP protein in a human liver. Primary was used at 0.6 ug/ml.

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Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunohistochemistry: 1:100, Western Blot: 0.5-2.0 ug/ml, Immunohistochemistry-Paraffin: 1:100, Simple Western: 10 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:10-1:500
• Reactivities: Human, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a C-terminal region of human SLIRP protein. [Entrez-Prot# AAX58600]
• Formulation: Tris-glycine, 150mM NaCl and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Peptide affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: SRA stem-loop interacting RNA binding protein
• Database Link:
  NP_112487
  Entrez Gene 81892 Human
  UniProt ID Q9GZT3
• Synonyms: C14orf156; DC50; PD04872