GADD45G Mouse Monoclonal Antibody [Clone ID: OTI2F12]

CAT#: TA505437

GADD45G mouse monoclonal antibody, clone OTI2F12 (formerly 2F12)

Product Images

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human GADD45G plasmid (RC201364, lane 2), mouse GADD45G plasmid (MR201228, lane 3) using anti-GADD45G antibody TA505437 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Figure from citation: Western Blot of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines. View Citation

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Anti-GADD45G mouse monoclonal antibody (TA505437) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G (RC201364).

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  Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines. View Citation

• Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human GBM8401 cells. View Citation

• Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human CRC cells. View Citation

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Specifications

Product Data

• Clone Name: OTI2F12
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GADD45G(NP_006696) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.9 kDa
• Gene Name: growth arrest and DNA damage inducible gamma
• Database Link:
  NP_006696
  Entrez Gene 23882 MouseEntrez Gene 291005 RatEntrez Gene 10912 Human
  UniProt ID O95257
• Background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008]
• Synonyms: CR6; DDIT2; GADD45gamma; GRP17

Reference Data

• Protein Pathways: Cell cycle, MAPK signaling pathway, p53 signaling pathway