GADD45G Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2F12]

CAT#: TA505437AM

GADD45G mouse monoclonal antibody,clone 2F12, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G. Positive lysates LY416476 (100ug) and LC416476 (20ug) can be purchased separately from OriGene.

• 

  Figure from citation: Western Blot of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines. View Citation

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505437)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505437)

• 

  Anti-GADD45G mouse monoclonal antibody (TA505437) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G (RC201364).

• 

  Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines. View Citation

• Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human GBM8401 cells. View Citation

• Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human CRC cells. View Citation

Show all 8 images

Specifications

Product Data

• Clone Name: OTI2F12
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GADD45G(NP_006696) produced in HEK293T cell.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.9 kDa
• Gene Name: growth arrest and DNA damage inducible gamma
• Database Link:
  NP_006696
  Entrez Gene 23882 MouseEntrez Gene 291005 RatEntrez Gene 10912 Human
  UniProt ID O95257
• Background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008]
• Synonyms: CR6; DDIT2; GADD45gamma; GRP17

Reference Data

• Protein Pathways: Cell cycle, MAPK signaling pathway, p53 signaling pathway