CRABP2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI10D6]

CAT#: TA800069BM

CRABP2 mouse monoclonal antibody,clone 10D6, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRABP2 (RC200221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRABP2. Positive lysates LY419677 (100ug) and LC419677 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRABP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRABP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800069)

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  HEK293T cells transfected with either RC200221 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRABP2 antibody (TA800069), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI10D6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CRABP2 (NP_001869) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15.5 kDa
• Gene Name: cellular retinoic acid binding protein 2
• Database Link:
  NP_001869
  Entrez Gene 12904 MouseEntrez Gene 1382 Human
  UniProt ID P29373
• Background: This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Synonyms: CRABP-II; RBP6

Reference Data

• Protein Families: Druggable Genome, Transcription Factors