IRAK4 (NM_016123) Human Tagged ORF Clone

CAT#: RC203041

IRAK4 (Myc-DDK-tagged)-Human interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY426473) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225772 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IRAK4 protein (Cat# TP303041). The protein was produced from HEK293T cells transfected with IRAK4 cDNA clone (Cat# RC203041) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRAK4
• Synonyms: IPD1; IRAK-4; NY-REN-64; REN64
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203041 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAATGTTGGACTAATTAGGAAGCTGTCAG
  ATTTTATTGATCCTCAAGAAGGATGGAAGAAGTTAGCTGTAGCTATTAAAAAACCATCTGGTGATGATAG
  ATACAATCAGTTTCACATAAGGAGATTTGAAGCATTACTTCAAACTGGAAAAAGTCCCACTTCTGAATTA
  CTGTTTGACTGGGGCACCACAAATTGCACAGTTGGTGATCTTGTGGATCTTTTGATCCAAAATGAATTTT
  TTGCTCCTGCAAGTCTTTTGCTCCCAGATGCTGTTCCCAAAACTGCTAATACACTACCTTCTAAAGAAGC
  TATAACAGTTCAGCAAAAACAGATGCCTTTCTGTGACAAAGACAGGACATTGATGACACCTGTGCAGAAT
  CTTGAACAAAGCTATATGCCACCTGACTCCTCAAGTCCAGAAAATAAAAGTTTAGAAGTTAGTGATACAC
  GTTTTCACAGTTTTTCATTTTATGAATTGAAGAATGTCACAAATAACTTTGATGAACGACCCATTTCTGT
  TGGTGGTAATAAAATGGGAGAGGGAGGATTTGGAGTTGTATATAAAGGCTACGTAAATAACACAACTGTG
  GCAGTGAAGAAGCTTGCAGCAATGGTTGACATTACTACTGAAGAACTGAAACAGCAGTTTGATCAAGAAA
  TAAAAGTAATGGCAAAGTGTCAACATGAAAACTTAGTAGAACTACTTGGTTTCTCAAGTGATGGAGATGA
  CCTCTGCTTAGTATATGTTTACATGCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGATGGTACT
  CCACCACTTTCTTGGCACATGAGATGCAAGATTGCTCAGGGTGCAGCTAATGGCATCAATTTTCTACATG
  AAAATCATCATATTCATAGAGATATTAAAAGTGCAAATATCTTACTGGATGAAGCTTTTACTGCTAAAAT
  ATCTGACTTTGGCCTTGCACGGGCTTCTGAGAAGTTTGCCCAGACAGTCATGACTAGCAGAATTGTGGGA
  ACAACAGCTTATATGGCACCAGAAGCTTTGCGTGGAGAAATAACACCCAAATCTGATATTTACAGCTTTG
  GTGTGGTTTTACTAGAAATAATAACTGGACTTCCAGCTGTGGATGAACACCGTGAACCTCAGTTATTGCT
  AGATATTAAAGAAGAAATTGAAGATGAAGAAAAGACAATTGAAGATTATATTGATAAAAAGATGAATGAT
  GCTGATTCCACTTCAGTTGAAGCTATGTACTCTGTTGCTAGTCAATGTCTGCATGAAAAGAAAAATAAGA
  GACCAGACATTAAGAAGGTTCAACAGCTGCTGCAAGAGATGACAGCTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203041 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSEL
  LFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQN
  LEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTV
  AVKKLAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVYMPNGSLLDRLSCLDGT
  PPLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDEAFTAKISDFGLARASEKFAQTVMTSRIVG
  TTAYMAPEALRGEITPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYIDKKMND
  ADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016123
• ORF Size: 1380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016123.1, NM_016123.2, NM_016123.3, NP_057207.1
• RefSeq Size: 4303 bp
• RefSeq ORF: 1383 bp
• Locus ID: 51135
• Domains: DEATH, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway, Toll-like receptor signaling pathway
• MW: 51.5 kDa
• Gene Summary: This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]