IRAK4 (NM_016123) Human Untagged Clone

CAT#: SC323327

IRAK4 (untagged)-Kinase deficient mutant (K213M) of Human interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRAK4
• Synonyms: IPD1; IRAK-4; NY-REN-64; REN64
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016123, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAATGTTGGACTAATTAGGAAGCTGTCAG
  ATTTTATTGATCCTCAAGAAGGATGGAAGAAGTTAGCTGTAGCTATTAAAAAACCATCTGGTGATGATAG
  ATACAATCAGTTTCACATAAGGAGATTTGAAGCATTACTTCAAACTGGAAAAAGTCCCACTTCTGAATTA
  CTGTTTGACTGGGGCACCACAAATTGCACAGTTGGTGATCTTGTGGATCTTTTGATCCAAAATGAATTTT
  TTGCTCCTGCGAGTCTTTTGCTCCCAGATGCTGTTCCCAAAACTGCTAATACACTACCTTCTAAAGAAGC
  TATAACAGTTCAGCAAAAACAGATGCCTTTCTGTGACAAAGACAGGACATTGATGACACCTGTGCAGAAT
  CTTGAACAAAGCTATATGCCACCTGACTCCTCAAGTCCAGAAAATAAAAGTTTAGAAGTTAGTGATACAC
  GTTTTCACAGTTTTTCATTTTATGAATTGAAGAATGTCACAAATAACTTTGATGAACGACCCATTTCTGT
  TGGTGGTAATAAAATGGGAGAGGGAGGATTTGGAGTTGTATATAAAGGCTACGTAAATAACACAACTGTG
  GCAGTGAAGAAGCTTGCAGCAATGGTTGACATTACTACTGAAGAACTGAAACAGCAGTTTGATCAAGAAA
  TAAAAGTAATGGCAAAGTGTCAACATGAAAACTTAGTAGAACTACTTGGTTTCTCAAGTGATGGAGATGA
  CCTCTGCTTAGTATATGTTTACATGCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGATGGTACT
  CCACCACTTTCTTGGCACATGAGATGCAAGATTGCTCAGGGTGCAGCTAATGGCATCAATTTTCTACATG
  AAAATCATCATATTCATAGAGATATTAAAAGTGCAAATATCTTACTGGATGAAGCTTTTACTGCTAAAAT
  ATCTGACTTTGGCCTTGCACGGGCTTCTGAGAAGTTTGCCCAGACAGTCATGACTAGCAGAATTGTGGGA
  ACAACAGCTTATATGGCACCAGAAGCTTTGCGTGGAGAAATAACACCCAAATCTGATATTTACAGCTTTG
  GTGTGGTTTTACTAGAAATAATAACTGGACTTCCAGCTGTGGATGAACACCGTGAACCTCAGTTATTGCT
  AGATATTAAAGAAGAAATTGAAGATGAAGAAAAGACAATTGAAGATTATATTGATAAAAAGATGAATGAT
  GCTGATTCCACTTCAGTTGAAGCTATGTACTCTGTTGCTAGTCAATGTCTGCATGAAAAGAAAAATAAGA
  GACCAGACATTAAGAAGGTTCAACAGCTGCTGCAAGAGATGACAGCTTCTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_016123 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGCCCGCGCCCG
  GGCAGGAATAGAAGATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAATGTTGGACTAAT
  TAGGAAGCTGTCAGATTTTATTGATCCTCAAGAAGGATGGAAGAAGTTAGCTGTAGCTATTAAAAAACCA
  TCTGGTGATGATAGATACAATCAGTTTCACATAAGATGCTGTTCCCAAAACTGCTAATACACTACCCTTC
  TAAAAGAAGCTATAACAGTTCAGCAAAAAACAGATGCCTTTCTGTGACAAAGACAGGACATTGATGACAC
  CTTGTGCAGATCCTTGACAAAGCTTATGCCACCTGACTCCTCAAGTCCGAAAATAAAAGTTAAAAGTAGT
  GAACACGTTTCCCAGTTTTCCATTTTATGATTTGAGATGGCCAAATTAACTTTGATGAAGGACCATTTTC
  GGTGGTGGTATAAATGGGGAAGGAGGGATTGGGATTTGTATAAAGGTCATTATAAACACCGGGGGATGAG
  AGACTTGCAAAGGGTTGACTTCTCTCGGAAACTAAACACGTTGTTCGAGATAAGTAGGGAAGGGAACTGA
  AACATATAAACTGGTTCTAGTGTGAGAGAGCCTGCTATATAGTACACGCAAGGCATCGGCAGACATCTCG
  TGGTGGTCTCCACTCTTGCCAGAGCCATGCCAGGACTAAGGACAATTCAGATATCACTCAGATATTGGCA
  ATTCGGAGCTTTTT
  
  Kinase domain sequence

  >SC323327 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ACGATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGCCCGCGCCCGGGCA
  GGAATAGAAGATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAATGTTGGACTAATTAGG
  AAGCTGTCAGATTTTATTGATCCTCAAGAAGGATGGAAGAAGTTA
  
• Restriction Sites: Please inquire
• ACCN: NM_016123
• ORF Size: 1383 bp
• Insert Size: 3000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_016123.1, NP_057207.1
• RefSeq Size: 2817
• RefSeq ORF: 1383
• Locus ID: 51135
• Domains: DEATH, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 13 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.