SAV1 (NM_021818) Human Tagged ORF Clone
CAT#: RC204389
SAV1 (Myc-DDK-tagged)-Human salvador homolog 1 (Drosophila) (SAV1)
"NM_021818" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SAV1 |
Synonyms | SAV; WW45; WWP4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204389 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCCCGAAAGAAAACCAAAAACGAAGTGTCCAAGCCGGCCGAGGTGCAGGGGAAGTACGTGAAGA AGGAGACGTCGCCTCTGCTTCGGAATCTTATGCCTTCATTCATCCGGCATGGTCCAACAATTCCAAGACG AACTGATATCTGTCTTCCAGATTCAAGCCCTAATGCCTTTTCAACTTCTGGAGATGTAGTTTCAAGAAAC CAGAGTTTCCTTAGAACTCCAATTCAAAGAACACCTCATGAAATAATGAGAAGAGAAAGCAACAGATTAT CTGCACCTTCTTATCTTGCCAGAAGTCTAGCAGATGTCCCTAGAGAGTATGGTTCTTCTCAGTCATTTGT AACGGAAGTTAGTTTTGCTGTTGAAAATGGAGACTCTGGTTCCCGATATTATTATTCAGACAATTTTTTT GATGGTCAGAGAAAGCGGCCACTTGGAGATCGTGCACATGAAGACTACAGATATTATGAATACAACCATG ATCTCTTCCAAAGAATGCCACAGAATCAGGGGAGGCATGCTTCAGGTATTGGGAGAGTTGCTGCTACATC TTTAGGAAATTTGACTAACCATGGTTCTGAAGATTTACCCCTTCCTCCTGGCTGGTCTGTGGACTGGACA ATGAGAGGGAGAAAATATTATATAGATCATAACACAAATACAACTCACTGGAGCCATCCTCTTGAGCGAG AAGGACTTCCTCCTGGATGGGAACGAGTTGAGTCATCCGAATTTGGAACCTATTATGTAGATCACACAAA TAAGAAGGCCCAATACAGGCATCCCTGTGCTCCTAGTGTACCTCGGTATGATCAACCACCTCCTGTCACA TACCAGCCACAGCAAACTGAAAGAAATCAGTCCCTTCTGGTACCTGCAAATCCATATCATACTGCAGAAA TTCCTGACTGGCTTCAGGTTTACGCACGAGCCCCTGTGAAATATGACCACATTCTGAAGTGGGAACTCTT CCAGCTGGCTGACCTGGATACATACCAGGGAATGCTAAAGTTGCTCTTCATGAAAGAATTGGAGCAGATT GTTAAAATGTATGAAGCATACAGACAAGCCCTTCTTACAGAGTTGGAAAACCGAAAGCAGAGACAGCAGT GGTATGCCCAACAACATGGAAAAAATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204389 protein sequence
Red=Cloning site Green=Tags(s) MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDICLPDSSPNAFSTSGDVVSRN QSFLRTPIQRTPHEIMRRESNRLSAPSYLARSLADVPREYGSSQSFVTEVSFAVENGDSGSRYYYSDNFF DGQRKRPLGDRAHEDYRYYEYNHDLFQRMPQNQGRHASGIGRVAATSLGNLTNHGSEDLPLPPGWSVDWT MRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVT YQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQI VKMYEAYRQALLTELENRKQRQQWYAQQHGKNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021818 |
ORF Size | 1149 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021818.1, NM_021818.2, NM_021818.3, NP_068590.1 |
RefSeq Size | 3141 bp |
RefSeq ORF | 1152 bp |
Locus ID | 60485 |
Domains | WW |
MW | 44.6 kDa |
Gene Summary | WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein with two WW domains, a SARAH domain, and a coiled-coil region and is ubiquitously expressed in adult tissues. This protein binds to MST1 (mammalian sterile 20-like kinase 1) and promotes MST1-induced apoptosis. It has also been shown to bind to HAX1 (hematopoietic cell-specific protein 1 (HS1)-associated protein X-1) and to attenuate the anti-apoptotic effects of HAX1. Studies in human and mouse suggest this gene acts as a tumor suppressor. [provided by RefSeq, Aug 2012] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC112869 | SAV1 (untagged)-Human salvador homolog 1 (Drosophila) (SAV1) |
USD 310.00 |
|
RG204389 | SAV1 (GFP-tagged) - Human salvador homolog 1 (Drosophila) (SAV1) |
USD 460.00 |
|
RC204389L3 | Lenti ORF clone of Human salvador homolog 1 (Drosophila) (SAV1), Myc-DDK-tagged |
USD 620.00 |
|
RC204389L4 | Lenti ORF clone of Human salvador homolog 1 (Drosophila) (SAV1), mGFP tagged |
USD 620.00 |
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