SAV1 (NM_021818) Human Tagged ORF Clone

CAT#: RG204389

SAV1 (GFP-tagged) - Human salvador homolog 1 (Drosophila) (SAV1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SAV1
• Synonyms: SAV; WW45; WWP4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204389 representing NM_021818
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCCGAAAGAAAACCAAAAACGAAGTGTCCAAGCCGGCCGAGGTGCAGGGGAAGTACGTGAAGA
  AGGAGACGTCGCCTCTGCTTCGGAATCTTATGCCTTCATTCATCCGGCATGGTCCAACAATTCCAAGACG
  AACTGATATCTGTCTTCCAGATTCAAGCCCTAATGCCTTTTCAACTTCTGGAGATGTAGTTTCAAGAAAC
  CAGAGTTTCCTTAGAACTCCAATTCAAAGAACACCTCATGAAATAATGAGAAGAGAAAGCAACAGATTAT
  CTGCACCTTCTTATCTTGCCAGAAGTCTAGCAGATGTCCCTAGAGAGTATGGTTCTTCTCAGTCATTTGT
  AACGGAAGTTAGTTTTGCTGTTGAAAATGGAGACTCTGGTTCCCGATATTATTATTCAGACAATTTTTTT
  GATGGTCAGAGAAAGCGGCCACTTGGAGATCGTGCACATGAAGACTACAGATATTATGAATACAACCATG
  ATCTCTTCCAAAGAATGCCACAGAATCAGGGGAGGCATGCTTCAGGTATTGGGAGAGTTGCTGCTACATC
  TTTAGGAAATTTGACTAACCATGGTTCTGAAGATTTACCCCTTCCTCCTGGCTGGTCTGTGGACTGGACA
  ATGAGAGGGAGAAAATATTATATAGATCATAACACAAATACAACTCACTGGAGCCATCCTCTTGAGCGAG
  AAGGACTTCCTCCTGGATGGGAACGAGTTGAGTCATCCGAATTTGGAACCTATTATGTAGATCACACAAA
  TAAGAAGGCCCAATACAGGCATCCCTGTGCTCCTAGTGTACCTCGGTATGATCAACCACCTCCTGTCACA
  TACCAGCCACAGCAAACTGAAAGAAATCAGTCCCTTCTGGTACCTGCAAATCCATATCATACTGCAGAAA
  TTCCTGACTGGCTTCAGGTTTACGCACGAGCCCCTGTGAAATATGACCACATTCTGAAGTGGGAACTCTT
  CCAGCTGGCTGACCTGGATACATACCAGGGAATGCTAAAGTTGCTCTTCATGAAAGAATTGGAGCAGATT
  GTTAAAATGTATGAAGCATACAGACAAGCCCTTCTTACAGAGTTGGAAAACCGAAAGCAGAGACAGCAGT
  GGTATGCCCAACAACATGGAAAAAATTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204389 representing NM_021818
  Red=Cloning site Green=Tags(s)
  
  MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDICLPDSSPNAFSTSGDVVSRN
  QSFLRTPIQRTPHEIMRRESNRLSAPSYLARSLADVPREYGSSQSFVTEVSFAVENGDSGSRYYYSDNFF
  DGQRKRPLGDRAHEDYRYYEYNHDLFQRMPQNQGRHASGIGRVAATSLGNLTNHGSEDLPLPPGWSVDWT
  MRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVT
  YQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQI
  VKMYEAYRQALLTELENRKQRQQWYAQQHGKNF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021818
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021818.2, NP_068590.1
• RefSeq Size: 3031
• RefSeq ORF: 1152
• Locus ID: 60485
• Domains: WW
• Gene Summary: WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein with two WW domains, a SARAH domain, and a coiled-coil region and is ubiquitously expressed in adult tissues. This protein binds to MST1 (mammalian sterile 20-like kinase 1) and promotes MST1-induced apoptosis. It has also been shown to bind to HAX1 (hematopoietic cell-specific protein 1 (HS1)-associated protein X-1) and to attenuate the anti-apoptotic effects of HAX1. Studies in human and mouse suggest this gene acts as a tumor suppressor. [provided by RefSeq, Aug 2012]