MED25 (NM_030973) Human Tagged ORF Clone

CAT#: RC209044

MED25 (Myc-DDK-tagged)-Human mediator complex subunit 25 (MED25)

Product Images

Western blot validation of overexpression lysate (Cat# LY410636) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209044 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MED25 protein (Cat# TP309044). The protein was produced from HEK293T cells transfected with MED25 cDNA clone (Cat# RC209044) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MED25
• Synonyms: ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2; TCBAP0758
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209044 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCCCGGGTCCGAGGGCCCGGCCCGCGCCGGGAGCGTGGTGGCCGACGTGGTGTTTGTGATTGAGG
  GTACGGCCAACCTGGGACCCTACTTCGAGGGGCTCCGCAAGCACTACCTGCTCCCGGCCATCGAGTATTT
  TAATGGTGGTCCTCCTGCTGAGACGGACTTCGGGGGAGACTATGGGGGGACCCAGTACAGCCTCGTGGTG
  TTCAACACAGTGGACTGCGCTCCCGAGTCCTACGTACAATGTCACGCTCCCACCAGCAGCGCCTATGAGT
  TTGTCACCTGGCTCGATGGCATTAAGTTCATGGGCGGGGGTGGTGAGAGCTGCAGCCTCATCGCGGAAGG
  ACTCAGCACAGCCTTGCAGCTGTTTGATGACTTCAAGAAGATGCGCGAGCAGATTGGCCAGACGCACCGG
  GTCTGCCTCCTCATCTGCAACTCACCCCCATACTTGTTGCCTGCTGTTGAGAGCACCACGTACTCTGGAT
  GCACAACTGAGAATCTTGTGCAGCAGATTGGGGAGCGGGGGATCCACTTCTCCATTGTGTCTCCCCGGAA
  GCTGCCTGCGCTTCGGCTTCTGTTTGAGAAGGCAGCCCCCCCGGCCTTGCTGGAGCCGCTGCAGCCTCCG
  ACAGATGTGAGCCAGGACCCGAGGCACATGGTGCTGGTTCGGGGACTCGTGCTGCCTGTTGGGGGTGGCT
  CAGCCCCAGGCCCCCTCCAGTCAAAGCAGCCAGTCCCCCTGCCTCCCGCCGCACCCTCAGGTGCCACTCT
  CTCAGCAGCCCCCCAGCAGCCTCTGCCCCCCGTCCCCCCGCAGTACCAGGTTCCCGGGAACCTGAGTGCA
  GCTCAGGTGGCCGCGCAGAATGCAGTGGAGGCTGCCAAGAACCAGAAGGCTGGGCTGGGCCCTCGCTTCT
  CGCCCATCACCCCTCTCCAACAAGCTGCTCCCGGAGTGGGTCCCCCCTTCAGCCAGGCCCCAGCTCCCCA
  ACTACCCCCAGGACCCCCTGGCGCCCCCAAGCCACCACCTGCTTCCCAGCCCAGTCTGGTCTCCACTGTG
  GCCCCTGGCTCCGGCCTGGCTCCCACGGCACAGCCCGGGGCACCGTCCATGGCAGGCACTGTGGCCCCAG
  GAGGGGTGAGCGGCCCTTCCCCAGCCCAGCTGGGAGCCCCAGCCCTCGGTGGGCAGCAGTCAGTCTCCAA
  TAAGCTTCTGGCCTGGAGCGGGGTCCTGGAGTGGCAAGAGAAACCCAAACCTGCCTCAGTGGATGCCAAC
  ACCAAGCTGACGCGGTCACTGCCCTGCCAGGTCTACGTGAATCATGGCGAGAACCTGAAGACGGAGCAGT
  GGCCCCAGAAGCTGATCATGCAGCTCATCCCCCAGCAGCTGCTGACCACCCTGGGCCCTTTGTTCCGGAA
  CTCAAGGATGGTCCAGTTCCATTTCACCAACAAGGACCTGGAGTCTCTCAAAGGCCTCTACCGCATCATG
  GGCAACGGCTTCGCGGGCTGCGTGCACTTCCCCCACACGGCGCCCTGTGAGGTGCGCGTGCTCATGCTCC
  TGTACTCGTCCAAGAAGAAGATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTCGTCAACGGCAT
  CCGGCAGGTCATCACCAACCACAAGCAGGTCCAGCAGCAGAAGCTGGAGCAGCAGCAGCGAGGAATGGGG
  GGACAGCAGGCACCCCCAGGGCTGGGGCCCATTCTGGAGGACCAAGCCAGGCCCTCACAGAATCTGCTCC
  AGCTCCGCCCACCGCAGCCCCAGCCTCAGGGTACCGTAGGGGCCTCTGGGGCCACGGGGCAGCCCCAGCC
  CCAAGGTACTGCCCAGCCCCCGCCAGGTGCCCCTCAAGGCCCTCCTGGAGCAGCTTCTGGCCCACCCCCT
  CCTGGACCCATCCTTCGGCCCCAGAACCCTGGGGCCAACCCTCAGCTGCGAAGCCTCCTCCTCAACCCAC
  CACCGCCGCAGACTGGGGTGCCCCCACCCCAGGCCTCCCTCCACCACCTCCAGCCACCAGGGGCTCCTGC
  GCTGCTGCCTCCGCCGCACCAGGGCCTGGGGCAGCCCCAGTTGGGGCCCCCACTCCTGCATCCACCACCT
  GCCCAGTCCTGGCCCGCACAACTTCCCCCTCGGGCTCCACTGCCAGGTCAGATGCTGCTGAGCGGGGGTC
  CCCGGGGCCCGGTCCCCCAGCCGGGCCTGCAGCCCAGCGTCATGGAGGACGACATCCTCATGGATCTCAT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209044 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVV
  FNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHR
  VCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPP
  TDVSQDPRHMVLVRGLVLPVGGGSAPGPLQSKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSA
  AQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTV
  APGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDAN
  TKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIM
  GNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMG
  GQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGAASGPPP
  PGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPP
  AQSWPAQLPPRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030973
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030973.2, NM_030973.3, NP_112235.2
• RefSeq Size: 2361
• RefSeq ORF: 2244
• Locus ID: 81857
• MW: 78.2 kDa
• Gene Summary: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010]