MED25 (NM_030973) Human Untagged Clone

CAT#: SC108991

MED25 (untagged)-Human mediator complex subunit 25 (MED25)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MED25
• Synonyms: ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2; TCBAP0758
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030973, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTCCCCGGGTCCGAGGGCCCGGCCCGCGCCGGGAGCGTGGTGGCCGACGTGGTGTTTGTGATTGAGG
  GTACGGCCAACCTGGGACCCTACTTCGAGGGGCTCCGCAAGCACTACCTGCTCCCGGCCATCGAGTATTT
  TAATGGTGGTCCTCCTGCTGAGACGGACTTCGGGGGAGACTATGGGGGGACCCAGTACAGCCTCGTGGTG
  TTCAACACAGTGGACTGCGCTCCCGAGTCCTACGTACAATGTCACGCTCCCACCAGCAGCGCCTATGAGT
  TTGTCACCTGGCTCGATGGCATTAAGTTCATGGGCGGGGGTGGTGAGAGCTGCAGCCTCATCGCGGAAGG
  ACTCAGCACAGCCTTGCAGCTGTTTGATGACTTCAAGAAGATGCGCGAGCAGATTGGCCAGACGCACCGG
  GTCTGCCTCCTCATCTGCAACTCACCCCCATACTTGTTGCCTGCTGTTGAGAGCACCACGTACTCTGGAT
  GCACAACTGAGAATCTTGTGCAGCAGATTGGGGAGCGGGGGATCCACTTCTCCATTGTGTCTCCCCGGAA
  GCTGCCTGCGCTTCGGCTTCTGTTTGAGAAGGCAGCCCCCCCGGCCTTGCTGGAGCCGCTGCAGCCTCCG
  ACAGATGTGAGCCAGGACCCGAGGCACATGGTGCTGGTTCGGGGACTCGTGCTGCCTGTTGGGGGTGGCT
  CAGCCCCAGGCCCCCTCCAGTCAAAGCAGCCAGTCCCCCTGCCTCCCGCCGCACCCTCAGGTGCCACTCT
  CTCAGCAGCCCCCCAGCAGCCTCTGCCCCCCGTCCCCCCGCAGTACCAGGTTCCCGGGAACCTGAGTGCA
  GCTCAGGTGGCCGCGCAGAATGCAGTGGAGGCTGCCAAGAACCAGAAGGCTGGGCTGGGCCCTCGCTTCT
  CGCCCATCACCCCTCTCCAACAAGCTGCTCCCGGAGTGGGTCCCCCCTTCAGCCAGGCCCCAGCTCCCCA
  ACTACCCCCAGGACCCCCTGGCGCCCCCAAGCCACCACCTGCTTCCCAGCCCAGTCTGGTCTCCACTGTG
  GCCCCTGGCTCCGGCCTGGCTCCCACGGCACAGCCCGGGGCACCGTCCATGGCAGGCACTGTGGCCCCAG
  GAGGGGTGAGCGGCCCTTCCCCAGCCCAGCTGGGAGCCCCAGCCCTCGGTGGGCAGCAGTCAGTCTCCAA
  TAAGCTTCTGGCCTGGAGCGGGGTCCTGGAGTGGCAAGAGAAACCCAAACCTGCCTCAGTGGATGCCAAC
  ACCAAGCTGACGCGGTCACTGCCCTGCCAGGTCTACGTGAATCATGGCGAGAACCTGAAGACGGAGCAGT
  GGCCCCAGAAGCTGATCATGCAGCTCATCCCCCAGCAGCTGCTGACCACCCTGGGCCCTTTGTTCCGGAA
  CTCAAGGATGGTCCAGTTCCATTTCACCAACAAGGACCTGGAGTCTCTCAAAGGCCTCTACCGCATCATG
  GGCAACGGCTTCGCGGGCTGCGTGCACTTCCCCCACACGGCGCCCTGTGAGGTGCGCGTGCTCATGCTCC
  TGTACTCGTCCAAGAAGAAGATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTCGTCAACGGCAT
  CCGGCAGGTCATCACCAACCACAAGCAGGTCCAGCAGCAGAAGCTGGAGCAGCAGCAGCGAGGAATGGGG
  GGACAGCAGGCACCCCCAGGGCTGGGGCCCATTCTGGAGGACCAAGCCAGGCCCTCACAGAATCTGCTCC
  AGCTCCGCCCACCGCAGCCCCAGCCTCAGGGTACCGTAGGGGCCTCTGGGGCCACGGGGCAGCCCCAGCC
  CCAAGGTACTGCCCAGCCCCCGCCAGGTGCCCCTCAAGGCCCTCCTGGAGCAGCTTCTGGCCCACCCCCT
  CCTGGACCCATCCTTCGGCCCCAGAACCCTGGGGCCAACCCTCAGCTGCGAAGCCTCCTCCTCAACCCAC
  CACCGCCGCAGACTGGGGTGCCCCCACCCCAGGCCTCCCTCCACCACCTCCAGCCACCAGGGGCTCCTGC
  GCTGCTGCCTCCGCCGCACCAGGGCCTGGGGCAGCCCCAGTTGGGGCCCCCACTCCTGCATCCACCACCT
  GCCCAGTCCTGGCCCGCACAACTTCCCCCTCGGGCTCCACTGCCAGGTCAGATGCTGCTGAGCGGGGGTC
  CCCGGGGCCCGGTCCCCCAGCCGGGCCTGCAGCCCAGCGTCATGGAGGACGACATCCTCATGGATCTCAT
  CTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030973 unedited
  TATAACACCCGCCCTGTTGAACGCAAATGGGCGGTAGGCGTGTACGGTGGGCAGGTCTAT
  ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
  GCCGCGAATTCGGCACGAGGGTGGCGGGTACCGCACGGGGTATGGTCCCCGGGTCCGAGG
  GCCCGGCCCGCGCCGGGAGCGTGGTGGCCGACGTGGTGTTTGTGATTGAGGGTACGGCCA
  ACCTGGGACCCTACTTCGAGGGGCTCCGCACGCACTACCTGCTCCCGGCCATCGAGTATT
  TTAATGGTGGTCCTCCTGCTGAGACGGACTTCGGGGGAGACTATGGGGGGACCCAGTACA
  GCCTCGTGGTGTTCAACACAGTGGACTGCGCTCCCGAGTCCTACGTACAATGTCACGCTC
  CCACCAGCAGCGCCTATGAGTTTGTCACCTGGCTCGATGGCATTAAGTTCATGGGCGGGG
  GTGGTGAGAGCTGCAGCCTCATCGCGGAAGGACTCAGCACAGCCTTGCAGCTGTTTGATG
  ACTTCAAGAAGATGCGCGAGCAGATTGGCCAGACGCACCGGGTCTGCCTCCTCATCTGCA
  ACTCACCCCCATACTTGTTGCCTGCTGTTGAGAGCACCACGTACTCTGGATGCACAACTG
  AGCATCTTGTGCAGCAGATTGGGGAGCGGNGGCACCACTTCTCCATTGNGTCTCCCCGCA
  GCTGCCTGCGCTTTCGCTTCTGATTGAGAGGCAGCCCCCTCGCCTTGCTGAGCGCTGCAC
  GCTCCGAAGATGTAGCTGGACCTGAGACAGGGGCTTGTTTGGGACTCGGCTGCTGTTGGG
  GGGGTTTAACCTAGGCCCTTTAGTAAGGAGCCGGCCCTTGCTTCCCCGCACCTAGGGGCA
  TTTTTTAAAGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030973 unedited
  TTTCCCTTGGGAATTAACTATGNACCCGCGGCCGCTTNCTANGATCGAGTTTTTTTTTTT
  TTTTTTTATCATCAAATCGGATCTTTACTCAACTATGGTGAATGCATGGAACATAATACA
  AACATGGATGTTACTGGAGTTTATATTTTTAAATTCAAAATTGAAAAAATACAAATTGAA
  AAATAGAAATATTTAATTATTAAAATTTTCTCTTTCATAATTCTTTTTGCGCCTTTGAGT
  TGAAGCCAAAAGGCTGGCGTCTTCCCTTCCACACCGAGATGTCAGTGTCAGGCCTCGGGT
  GGCACGGTGGGGACGGGCAGTTTAGAATTCAGCCTTGAATCCTTCAACTGGGAACGATGT
  TTAATTTGATTCAGGTTCGTAACGGAAAACAGCTGTTCACAAGCGTGGGTACTCCCAAAC
  ACAGATATTCAGGACAAACTACTGGAGATAGTTGTAGAATCTCACCATCTTCACACAGAC
  TTGTATTTAGGAACCAGCGCCGTATTGCACTGTAGTTCAATTACTATTTATGGTCTGCAT
  TCACACAAGAAACATCTGCCAACAAAGGCAAACTGGACAGTGTCTGTTCCTTCTCATGAG
  TCTGGATTCCGAGAGCCTTTGGGGGAATTTGGTCTTCAACCCCAGGACGTAGGTGAATAC
  AGTCCGGGCCACCTCTTTCATTTCTGAAAGCCGACTGCAGCACAGGAAAGAGTAAAGGTT
  TTTTTTCCTTGCCGGTGGATTCCCCAGGACCAAGCATACACCATCATACATTCAAGTAGC
  TGTTTAGGGACATCCTTGCGGAGAAATATTCAGTGTTCAGAACGGTCATAACCTTGACCA
  GAAGTCTTTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030973
• ORF Size: 2244 bp
• Insert Size: 4700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_030973.2, NP_112235.2
• RefSeq Size: 2335
• RefSeq ORF: 2244
• Locus ID: 81857
• Gene Summary: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010]