MED25 (NM_030973) Human Tagged ORF Clone
CAT#: RC209044L1
- LentiORF®
Lenti ORF clone of Human mediator complex subunit 25 (MED25), Myc-DDK-tagged
"NM_030973" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MED25 |
Synonyms | ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2; TCBAP0758 |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC209044).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_030973 |
ORF Size | 2241 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030973.2 |
RefSeq Size | 2361 bp |
RefSeq ORF | 2244 bp |
Locus ID | 81857 |
UniProt ID | Q71SY5 |
Cytogenetics | 19q13.33 |
MW | 78.2 kDa |
Gene Summary | This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108991 | MED25 (untagged)-Human mediator complex subunit 25 (MED25) |
USD 310.00 |
|
SC321957 | MED25 (untagged)-Human mediator complex subunit 25 (MED25) |
USD 310.00 |
|
RC209044 | MED25 (Myc-DDK-tagged)-Human mediator complex subunit 25 (MED25) |
USD 610.00 |
|
RG209044 | MED25 (GFP-tagged) - Human mediator complex subunit 25 (MED25) |
USD 670.00 |
|
RC209044L2 | Lenti ORF clone of Human mediator complex subunit 25 (MED25), mGFP tagged |
USD 810.00 |
|
RC209044L3 | Lenti ORF clone of Human mediator complex subunit 25 (MED25), Myc-DDK-tagged |
USD 810.00 |
|
RC209044L4 | Lenti ORF clone of Human mediator complex subunit 25 (MED25), mGFP tagged |
USD 810.00 |
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