DROSHA (NM_013235) Human Tagged ORF Clone
CAT#: RC213916
DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 1
"NM_013235" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | DROSHA |
Synonyms | ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213916 representing NM_013235
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCAGGGAAACACATGTCACAGAATGTCGTTCCACCCGGGACGAGGGTGTCCCCGAGGACGAGGAG GACATGGAGCCAGACCCTCAGCACCATCCTTTAGGCCCCAAAATCTGAGGCTGCTTCACCCTCAGCAGCC TCCTGTGCAATATCAATATGAACCTCCAAGTGCCCCTTCCACCACTTTCTCAAACTCTCCAGCCCCCAAT TTTCTCCCTCCACGACCAGACTTTGTACCCTTCCCCCCACCCATGCCTCCGTCAGCGCAAGGCCCTCTTC CCCCCTGCCCAATCAGGCCGCCTTTCCCCAACCACCAGATGAGGCACCCCTTCCCAGTTCCTCCTTGTTT TCCTCCCATGCCACCACCAATGCCTTGTCCTAATAACCCCCCAGTCCCTGGGGCACCTCCTGGACAAGGC ACTTTCCCCTTCATGATGCCCCCTCCCTCCATGCCTCATCCCCCGCCCCCTCCAGTCATGCCGCAGCAGG TTAATTATCAGTACCCTCCGGGCTATTCTCACCACAACTTCCCACCTCCCAGTTTTAATAGTTTCCAGAA CAACCCTAGTTCTTTCCTGCCCAGTGCTAATAACAGCAGTAGTCCTCATTTCAGACATCTCCCTCCATAC CCACTCCCAAAGGCTCCCAGTGAGAGAAGGTCCCCAGAAAGGCTGAAACACTATGATGACCACAGGCACC GAGATCACAGTCATGGGCGAGGTGAGAGGCATCGGTCCCTGGATCGGCGGGAGCGAGGCCGCAGTCCCGA CAGGAGAAGACAAGACAGCCGGTACAGATCTGATTATGACCGAGGGAGAACACCATCTCGCCACCGCAGC TACGAACGGAGCAGAGAGCGAGAACGGGAGAGACACAGGCATCGAGACAACCGAAGATCACCATCTCTGG AAAGGTCCTACAAAAAAGAGTATAAGAGATCTGGAAGGAGTTACGGTTTATCGGTTGTTCCTGAACCTGC TGGATGCACACCAGAATTACCTGGGGAGATTATTAAAAATACAGATTCTTGGGCCCCACCCCTGGAGATT GTGAATCATCGCTCCCCAAGTAGGGAGAAGAAGAGAGCTCGTTGGGAGGAAGAAAAAGACCGTTGGAGTG ACAACCAGAGTTCTGGCAAAGACAAGAACTATACCTCAATCAAGGAAAAAGAGCCCGAGGAGACCATGCC TGACAAGAATGAGGAGGAAGAAGAAGAACTTCTTAAGCCTGTGTGGATTCGATGCACTCATTCAGAAAAC TACTACTCCAGTGACCCCATGGATCAGGTGGGAGATTCTACAGTGGTTGGAACGAGTAGGCTTCGTGACT TATATGACAAATTTGAGGAGGAGTTGGGGAGCAGGCAAGAAAAGGCCAAAGCTGCTCGGCCTCCGTGGGA ACCTCCAAAGACGAAGCTCGATGAAGATTTAGAGAGTTCCAGTGAATCCGAGTGTGAGTCTGATGAGGAC AGCACCTGTTCTAGCAGCTCAGACTCTGAAGTTTTTGACGTTATTGCAGAAATCAAACGCAAAAAGGCCC ACCCTGACCGACTTCATGATGAACTTTGGTACAACGATCCAGGCCAGATGAATGATGGACCACTCTGCAA ATGCAGCGCAAAGGCAAGACGCACAGGAATTAGGCACAGCATTTATCCTGGAGAAGAGGCCATCAAGCCC TGTCGTCCTATGACCAACAATGCTGGCAGACTTTTCCACTACCGGATCACAGTCTCCCCGCCTACGAACT TTTTAACTGACAGGCCAACTGTTATAGAATACGATGATCACGAGTATATCTTTGAAGGATTTTCTATGTT TGCACATGCCCCCCTGACCAATATTCCACTGTGTAAAGTAATTAGATTCAACATAGACTACACGATTCAT TTCATTGAAGAGATGATGCCGGAGAATTTTTGTGTGAAAGGGCTTGAACTCTTTTCACTGTTCCTATTCA GAGATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGCCC AAGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGAAAGGAAGTGCTGTCCATGCAC CAGATTCTCCTGTACTTGTTAAGGTGCAGCAAAGCCCTGGTGCCTGAGGAGGAGATTGCCAATATGCTTC AGTGGGAGGAGCTGGAGTGGCAGAAATATGCAGAAGAATGCAAAGGCATGATTGTTACCAACCCTGGGAC GAAACCAAGCTCTGTCCGTATCGATCAACTGGATCGTGAACAGTTCAACCCCGATGTGATTACTTTTCCG ATTATCGTCCACTTTGGGATACGCCCTGCACAGTTGAGTTATGCAGGAGACCCACAGTACCAAAAACTGT GGAAGAGTTATGTGAAACTTCGCCACCTCCTAGCAAATAGTCCCAAAGTCAAACAAACTGACAAACAGAA GCTGGCACAGAGGGAGGAAGCCCTCCAAAAAATACGGCAGAAGAATACAATGAGACGAGAAGTAACGGTG GAGCTAAGTAGCCAAGGATTCTGGAAAACTGGCATCCGTTCTGATGTCTGTCAGCATGCAATGATGCTAC CTGTTCTGACCCATCATATCCGCTACCACCAATGCCTAATGCATTTGGACAAGTTGATAGGATATACTTT CCAAGATCGTTGTCTGTTGCAGCTGGCCATGACTCATCCAAGTCATCATTTAAATTTTGGAATGAATCCT GATCATGCCAGGAATTCATTATCTAACTGTGGAATTCGGCAGCCCAAATACGGAGACAGAAAAGTTCATC ACATGCACATGCGGAAGAAAGGGATTAACACCTTGATAAATATCATGTCACGCCTTGGCCAAGATGACCC AACTCCCTCGAGGATTAACCACAATGAACGGTTGGAATTCCTGGGTGATGCTGTTGTTGAATTTCTGACC AGCGTCCATTTGTACTATTTGTTTCCTAGTCTGGAAGAAGGAGGATTAGCAACCTATCGGACTGCCATTG TTCAGAATCAGCACCTTGCCATGCTAGCAAAGAAACTTGAACTGGATCCATTTATGCTGTATGCTCACGG GCCTGACCTTTGTAGAGAATCGGACCTTCGACATGCAATGGCCAATTGTTTTGAAGCGTTAATAGGAGCT GTTTACTTGGAGGGAAGCCTGGAGGAAGCCAAGCAGTTATTTGGACGCTTGCTCTTTAATGATCCGGACC TGCGCGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACTGATCGACAACT TATTGAAACTTCTCCAGTTCTACAAAAACTTACTGAGTTTGAAGAAGCAATTGGAGTAATTTTTACTCAT GTTCGACTTCTGGCAAGGGCATTCACATTGAGAACTGTGGGATTTAACCATCTGACCCTAGGCCACAATC AGAGAATGGAATTCCTAGGTGACTCCATAATGCAACTGGTAGCCACAGAGTACTTATTCATTCATTTCCC AGATCATCATGAAGGACACTTAACTTTGTTGCGAAGCTCTTTGGTGAATAATAGAACTCAGGCCAAGGTA GCGGAGGAGCTGGGCATGCAGGAGTACGCCATAACCAACGACAAGACCAAGAGGCCTGTGGCGCTTCGCA CCAAGACCTTGGCGGACCTTTTGGAATCATTTATTGCAGCGCTGTACACTGATAAGGATTTGGAATATGT TCATACTTTCATGAATGTCTGCTTCTTTCCACGATTGAAAGAATTCATTTTGAATCAGGATTGGAATGAC CCCAAATCCCAGCTTCAGCAGTGTTGCTTGACACTTAGGACAGAAGGAAAAGAGCCAGACATTCCTCTGT ACAAGACTCTGCAGACAGTGGGCCCATCCCATGCCCGAACCTACACTGTGGCTGTTTATTTCAAGGGAGA AAGAATAGGCTGTGGGAAAGGACCAAGTATTCAGCAAGCGGAAATGGGAGCAGCAATGGATGCGCTTGAA AAATATAATTTTCCCCAGATGGCCCATCAGAAGCGGTTCATCGAACGGAAGTACAGACAAGAGTTAAAAG AAATGAGGTGGGAAAGAGAGCATCAAGAGAGAGAGCCAGATGAGACTGAAGACATCAAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213916 representing NM_013235
Red=Cloning site Green=Tags(s) MMQGNTCHRMSFHPGRGCPRGRGGHGARPSAPSFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPN FLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRHPFPVPPCFPPMPPPMPCPNNPPVPGAPPGQG TFPFMMPPPSMPHPPPPPVMPQQVNYQYPPGYSHHNFPPPSFNSFQNNPSSFLPSANNSSSPHFRHLPPY PLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGRTPSRHRS YERSRERERERHRHRDNRRSPSLERSYKKEYKRSGRSYGLSVVPEPAGCTPELPGEIIKNTDSWAPPLEI VNHRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSEN YYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDED STCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKP CRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIH FIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMH QILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPDVITFP IIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTV ELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNP DHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLT SVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDPFMLYAHGPDLCRESDLRHAMANCFEALIGA VYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTH VRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKV AEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYTDKDLEYVHTFMNVCFFPRLKEFILNQDWND PKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALE KYNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPDETEDIKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013235 |
ORF Size | 4122 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_013235.2, NM_013235.3, NM_013235.4, NP_037367.2, NP_037367.3 |
RefSeq Size | 4764 |
RefSeq ORF | 4125 |
Locus ID | 29102 |
Domains | RIBOc, DSRM |
MW | 159.1 kDa |
Gene Summary | This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC308939 | DROSHA (untagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 1 |
USD 2,790.00 |
|
RG213916 | DROSHA (GFP-tagged) - Human drosha, ribonuclease type III (DROSHA), transcript variant 1 |
USD 1,580.00 |
|
RC213916L1 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, Myc-DDK-tagged |
USD 1,640.00 |
|
RC213916L2 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, mGFP tagged |
USD 1,640.00 |
|
RC213916L3 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, Myc-DDK-tagged |
USD 1,640.00 |
|
RC213916L4 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, mGFP tagged |
USD 2,028.00 |
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