DROSHA (NM_013235) Human Tagged ORF Clone

CAT#: RC213916L3

Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, Myc-DDK-tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DROSHA
• Synonyms: ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN
• Vector: pLenti-C-Myc-DDK-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC213916).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_013235
• ORF Size: 4122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013235.2, NP_037367.2, NP_037367.3
• RefSeq Size: 4764
• RefSeq ORF: 4125
• Locus ID: 29102
• Domains: RIBOc, DSRM
• MW: 159.1 kDa
• Gene Summary: This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]