DROSHA (NM_013235) Human Tagged ORF Clone

CAT#: RG213916

DROSHA (GFP-tagged) - Human drosha, ribonuclease type III (DROSHA), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DROSHA
• Synonyms: ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213916 representing NM_013235
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAGGGAAACACATGTCACAGAATGTCGTTCCACCCGGGACGAGGGTGTCCCCGAGGACGAGGAG
  GACATGGAGCCAGACCCTCAGCACCATCCTTTAGGCCCCAAAATCTGAGGCTGCTTCACCCTCAGCAGCC
  TCCTGTGCAATATCAATATGAACCTCCAAGTGCCCCTTCCACCACTTTCTCAAACTCTCCAGCCCCCAAT
  TTTCTCCCTCCACGACCAGACTTTGTACCCTTCCCCCCACCCATGCCTCCGTCAGCGCAAGGCCCTCTTC
  CCCCCTGCCCAATCAGGCCGCCTTTCCCCAACCACCAGATGAGGCACCCCTTCCCAGTTCCTCCTTGTTT
  TCCTCCCATGCCACCACCAATGCCTTGTCCTAATAACCCCCCAGTCCCTGGGGCACCTCCTGGACAAGGC
  ACTTTCCCCTTCATGATGCCCCCTCCCTCCATGCCTCATCCCCCGCCCCCTCCAGTCATGCCGCAGCAGG
  TTAATTATCAGTACCCTCCGGGCTATTCTCACCACAACTTCCCACCTCCCAGTTTTAATAGTTTCCAGAA
  CAACCCTAGTTCTTTCCTGCCCAGTGCTAATAACAGCAGTAGTCCTCATTTCAGACATCTCCCTCCATAC
  CCACTCCCAAAGGCTCCCAGTGAGAGAAGGTCCCCAGAAAGGCTGAAACACTATGATGACCACAGGCACC
  GAGATCACAGTCATGGGCGAGGTGAGAGGCATCGGTCCCTGGATCGGCGGGAGCGAGGCCGCAGTCCCGA
  CAGGAGAAGACAAGACAGCCGGTACAGATCTGATTATGACCGAGGGAGAACACCATCTCGCCACCGCAGC
  TACGAACGGAGCAGAGAGCGAGAACGGGAGAGACACAGGCATCGAGACAACCGAAGATCACCATCTCTGG
  AAAGGTCCTACAAAAAAGAGTATAAGAGATCTGGAAGGAGTTACGGTTTATCGGTTGTTCCTGAACCTGC
  TGGATGCACACCAGAATTACCTGGGGAGATTATTAAAAATACAGATTCTTGGGCCCCACCCCTGGAGATT
  GTGAATCATCGCTCCCCAAGTAGGGAGAAGAAGAGAGCTCGTTGGGAGGAAGAAAAAGACCGTTGGAGTG
  ACAACCAGAGTTCTGGCAAAGACAAGAACTATACCTCAATCAAGGAAAAAGAGCCCGAGGAGACCATGCC
  TGACAAGAATGAGGAGGAAGAAGAAGAACTTCTTAAGCCTGTGTGGATTCGATGCACTCATTCAGAAAAC
  TACTACTCCAGTGACCCCATGGATCAGGTGGGAGATTCTACAGTGGTTGGAACGAGTAGGCTTCGTGACT
  TATATGACAAATTTGAGGAGGAGTTGGGGAGCAGGCAAGAAAAGGCCAAAGCTGCTCGGCCTCCGTGGGA
  ACCTCCAAAGACGAAGCTCGATGAAGATTTAGAGAGTTCCAGTGAATCCGAGTGTGAGTCTGATGAGGAC
  AGCACCTGTTCTAGCAGCTCAGACTCTGAAGTTTTTGACGTTATTGCAGAAATCAAACGCAAAAAGGCCC
  ACCCTGACCGACTTCATGATGAACTTTGGTACAACGATCCAGGCCAGATGAATGATGGACCACTCTGCAA
  ATGCAGCGCAAAGGCAAGACGCACAGGAATTAGGCACAGCATTTATCCTGGAGAAGAGGCCATCAAGCCC
  TGTCGTCCTATGACCAACAATGCTGGCAGACTTTTCCACTACCGGATCACAGTCTCCCCGCCTACGAACT
  TTTTAACTGACAGGCCAACTGTTATAGAATACGATGATCACGAGTATATCTTTGAAGGATTTTCTATGTT
  TGCACATGCCCCCCTGACCAATATTCCACTGTGTAAAGTAATTAGATTCAACATAGACTACACGATTCAT
  TTCATTGAAGAGATGATGCCGGAGAATTTTTGTGTGAAAGGGCTTGAACTCTTTTCACTGTTCCTATTCA
  GAGATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGCCC
  AAGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGAAAGGAAGTGCTGTCCATGCAC
  CAGATTCTCCTGTACTTGTTAAGGTGCAGCAAAGCCCTGGTGCCTGAGGAGGAGATTGCCAATATGCTTC
  AGTGGGAGGAGCTGGAGTGGCAGAAATATGCAGAAGAATGCAAAGGCATGATTGTTACCAACCCTGGGAC
  GAAACCAAGCTCTGTCCGTATCGATCAACTGGATCGTGAACAGTTCAACCCCGATGTGATTACTTTTCCG
  ATTATCGTCCACTTTGGGATACGCCCTGCACAGTTGAGTTATGCAGGAGACCCACAGTACCAAAAACTGT
  GGAAGAGTTATGTGAAACTTCGCCACCTCCTAGCAAATAGTCCCAAAGTCAAACAAACTGACAAACAGAA
  GCTGGCACAGAGGGAGGAAGCCCTCCAAAAAATACGGCAGAAGAATACAATGAGACGAGAAGTAACGGTG
  GAGCTAAGTAGCCAAGGATTCTGGAAAACTGGCATCCGTTCTGATGTCTGTCAGCATGCAATGATGCTAC
  CTGTTCTGACCCATCATATCCGCTACCACCAATGCCTAATGCATTTGGACAAGTTGATAGGATATACTTT
  CCAAGATCGTTGTCTGTTGCAGCTGGCCATGACTCATCCAAGTCATCATTTAAATTTTGGAATGAATCCT
  GATCATGCCAGGAATTCATTATCTAACTGTGGAATTCGGCAGCCCAAATACGGAGACAGAAAAGTTCATC
  ACATGCACATGCGGAAGAAAGGGATTAACACCTTGATAAATATCATGTCACGCCTTGGCCAAGATGACCC
  AACTCCCTCGAGGATTAACCACAATGAACGGTTGGAATTCCTGGGTGATGCTGTTGTTGAATTTCTGACC
  AGCGTCCATTTGTACTATTTGTTTCCTAGTCTGGAAGAAGGAGGATTAGCAACCTATCGGACTGCCATTG
  TTCAGAATCAGCACCTTGCCATGCTAGCAAAGAAACTTGAACTGGATCCATTTATGCTGTATGCTCACGG
  GCCTGACCTTTGTAGAGAATCGGACCTTCGACATGCAATGGCCAATTGTTTTGAAGCGTTAATAGGAGCT
  GTTTACTTGGAGGGAAGCCTGGAGGAAGCCAAGCAGTTATTTGGACGCTTGCTCTTTAATGATCCGGACC
  TGCGCGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACTGATCGACAACT
  TATTGAAACTTCTCCAGTTCTACAAAAACTTACTGAGTTTGAAGAAGCAATTGGAGTAATTTTTACTCAT
  GTTCGACTTCTGGCAAGGGCATTCACATTGAGAACTGTGGGATTTAACCATCTGACCCTAGGCCACAATC
  AGAGAATGGAATTCCTAGGTGACTCCATAATGCAACTGGTAGCCACAGAGTACTTATTCATTCATTTCCC
  AGATCATCATGAAGGACACTTAACTTTGTTGCGAAGCTCTTTGGTGAATAATAGAACTCAGGCCAAGGTA
  GCGGAGGAGCTGGGCATGCAGGAGTACGCCATAACCAACGACAAGACCAAGAGGCCTGTGGCGCTTCGCA
  CCAAGACCTTGGCGGACCTTTTGGAATCATTTATTGCAGCGCTGTACACTGATAAGGATTTGGAATATGT
  TCATACTTTCATGAATGTCTGCTTCTTTCCACGATTGAAAGAATTCATTTTGAATCAGGATTGGAATGAC
  CCCAAATCCCAGCTTCAGCAGTGTTGCTTGACACTTAGGACAGAAGGAAAAGAGCCAGACATTCCTCTGT
  ACAAGACTCTGCAGACAGTGGGCCCATCCCATGCCCGAACCTACACTGTGGCTGTTTATTTCAAGGGAGA
  AAGAATAGGCTGTGGGAAAGGACCAAGTATTCAGCAAGCGGAAATGGGAGCAGCAATGGATGCGCTTGAA
  AAATATAATTTTCCCCAGATGGCCCATCAGAAGCGGTTCATCGAACGGAAGTACAGACAAGAGTTAAAAG
  AAATGAGGTGGGAAAGAGAGCATCAAGAGAGAGAGCCAGATGAGACTGAAGACATCAAGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213916 representing NM_013235
  Red=Cloning site Green=Tags(s)
  
  MMQGNTCHRMSFHPGRGCPRGRGGHGARPSAPSFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPN
  FLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRHPFPVPPCFPPMPPPMPCPNNPPVPGAPPGQG
  TFPFMMPPPSMPHPPPPPVMPQQVNYQYPPGYSHHNFPPPSFNSFQNNPSSFLPSANNSSSPHFRHLPPY
  PLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGRTPSRHRS
  YERSRERERERHRHRDNRRSPSLERSYKKEYKRSGRSYGLSVVPEPAGCTPELPGEIIKNTDSWAPPLEI
  VNHRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSEN
  YYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDED
  STCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKP
  CRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIH
  FIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMH
  QILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPDVITFP
  IIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTV
  ELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNP
  DHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLT
  SVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDPFMLYAHGPDLCRESDLRHAMANCFEALIGA
  VYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTH
  VRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKV
  AEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYTDKDLEYVHTFMNVCFFPRLKEFILNQDWND
  PKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALE
  KYNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPDETEDIKK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013235
• ORF Size: 4122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013235.2, NP_037367.2
• RefSeq Size: 4764
• RefSeq ORF: 4125
• Locus ID: 29102
• Domains: RIBOc, DSRM
• Gene Summary: This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]