DROSHA (NM_013235) Human Untagged Clone

CAT#: SC308939

DROSHA (untagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DROSHA
• Synonyms: ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013235, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGCAGGGAAACACATGTCACAGAATGTCGTTCCACCCGGGACGAGGGTGTCCCCGAGGACGAGGAG
  GACATGGAGCCAGACCCTCAGCACCATCCTTTAGGCCCCAAAATCTGAGGCTGCTTCACCCTCAGCAGCC
  TCCTGTGCAATATCAATATGAACCTCCAAGTGCCCCTTCCACCACTTTCTCAAACTCTCCAGCCCCCAAT
  TTTCTCCCTCCACGACCAGACTTTGTACCCTTCCCCCCACCCATGCCTCCGTCAGCGCAAGGCCCTCTTC
  CCCCCTGCCCAATCAGGCCGCCTTTCCCCAACCACCAGATGAGGCACCCCTTCCCAGTTCCTCCTTGTTT
  TCCTCCCATGCCACCACCAATGCCTTGTCCTAATAACCCCCCAGTCCCTGGGGCACCTCCTGGACAAGGC
  ACTTTCCCCTTCATGATGCCCCCTCCCTCCATGCCTCATCCCCCGCCCCCTCCAGTCATGCCGCAGCAGG
  TTAATTATCAGTACCCTCCGGGCTATTCTCACCACAACTTCCCACCTCCCAGTTTTAATAGTTTCCAGAA
  CAACCCTAGTTCTTTCCTGCCCAGTGCTAATAACAGCAGTAGTCCTCATTTCAGACATCTCCCTCCATAC
  CCACTCCCAAAGGCTCCCAGTGAGAGAAGGTCCCCAGAAAGGCTGAAACACTATGATGACCACAGGCACC
  GAGATCACAGTCATGGGCGAGGTGAGAGGCATCGGTCCCTGGATCGGCGGGAGCGAGGCCGCAGTCCCGA
  CAGGAGAAGACAAGACAGCCGGTACAGATCTGATTATGACCGAGGGAGAACACCATCTCGCCACCGCAGC
  TACGAACGGAGCAGAGAGCGAGAACGGGAGAGACACAGGCATCGAGACAACCGAAGATCACCATCTCTGG
  AAAGGTCCTACAAAAAAGAGTATAAGAGATCTGGAAGGAGTTACGGTTTATCGGTTGTTCCTGAACCTGC
  TGGATGCACACCAGAATTACCTGGGGAGATTATTAAAAATACAGATTCTTGGGCCCCACCCCTGGAGATT
  GTGAATCATCGCTCCCCAAGTAGGGAGAAGAAGAGAGCTCGTTGGGAGGAAGAAAAAGACCGTTGGAGTG
  ACAACCAGAGTTCTGGCAAAGACAAGAACTATACCTCAATCAAGGAAAAAGAGCCCGAGGAGACCATGCC
  TGACAAGAATGAGGAGGAAGAAGAAGAACTTCTTAAGCCTGTGTGGATTCGATGCACTCATTCAGAAAAC
  TACTACTCCAGTGACCCCATGGATCAGGTGGGAGATTCTACAGTGGTTGGAACGAGTAGGCTTCGTGACT
  TATATGACAAATTTGAGGAGGAGTTGGGGAGCAGGCAAGAAAAGGCCAAAGCTGCTCGGCCTCCGTGGGA
  ACCTCCAAAGACGAAGCTCGATGAAGATTTAGAGAGTTCCAGTGAATCCGAGTGTGAGTCTGATGAGGAC
  AGCACCTGTTCTAGCAGCTCAGACTCTGAAGTTTTTGACGTTATTGCAGAAATCAAACGCAAAAAGGCCC
  ACCCTGACCGACTTCATGATGAACTTTGGTACAACGATCCAGGCCAGATGAATGATGGACCACTCTGCAA
  ATGCAGCGCAAAGGCAAGACGCACAGGAATTAGGCACAGCATTTATCCTGGAGAAGAGGCCATCAAGCCC
  TGTCGTCCTATGACCAACAATGCTGGCAGACTTTTCCACTACCGGATCACAGTCTCCCCGCCTACGAACT
  TTTTAACTGACAGGCCAACTGTTATAGAATACGATGATCACGAGTATATCTTTGAAGGATTTTCTATGTT
  TGCACATGCCCCCCTGACCAATATTCCACTGTGTAAAGTAATTAGATTCAACATAGACTACACGATTCAT
  TTCATTGAAGAGATGATGCCGGAGAATTTTTGTGTGAAAGGGCTTGAACTCTTTTCACTGTTCCTATTCA
  GAGATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGCCC
  AAGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGAAAGGAAGTGCTGTCCATGCAC
  CAGATTCTCCTGTACTTGTTAAGGTGCAGCAAAGCCCTGGTGCCTGAGGAGGAGATTGCCAATATGCTTC
  AGTGGGAGGAGCTGGAGTGGCAGAAATATGCAGAAGAATGCAAAGGCATGATTGTTACCAACCCTGGGAC
  GAAACCAAGCTCTGTCCGTATCGATCAACTGGATCGTGAACAGTTCAACCCCGATGTGATTACTTTTCCG
  ATTATCGTCCACTTTGGGATACGCCCTGCACAGTTGAGTTATGCAGGAGACCCACAGTACCAAAAACTGT
  GGAAGAGTTATGTGAAACTTCGCCACCTCCTAGCAAATAGTCCCAAAGTCAAACAAACTGACAAACAGAA
  GCTGGCACAGAGGGAGGAAGCCCTCCAAAAAATACGGCAGAAGAATACAATGAGACGAGAAGTAACGGTG
  GAGCTAAGTAGCCAAGGATTCTGGAAAACTGGCATCCGTTCTGATGTCTGTCAGCATGCAATGATGCTAC
  CTGTTCTGACCCATCATATCCGCTACCACCAATGCCTAATGCATTTGGACAAGTTGATAGGATATACTTT
  CCAAGATCGTTGTCTGTTGCAGCTGGCCATGACTCATCCAAGTCATCATTTAAATTTTGGAATGAATCCT
  GATCATGCCAGGAATTCATTATCTAACTGTGGAATTCGGCAGCCCAAATACGGAGACAGAAAAGTTCATC
  ACATGCACATGCGGAAGAAAGGGATTAACACCTTGATAAATATCATGTCACGCCTTGGCCAAGATGACCC
  AACTCCCTCGAGGATTAACCACAATGAACGGTTGGAATTCCTGGGTGATGCTGTTGTTGAATTTCTGACC
  AGCGTCCATTTGTACTATTTGTTTCCTAGTCTGGAAGAAGGAGGATTAGCAACCTATCGGACTGCCATTG
  TTCAGAATCAGCACCTTGCCATGCTAGCAAAGAAACTTGAACTGGATCGATTTATGCTGTATGCTCACGG
  GCCTGACCTTTGTAGAGAATCGGACCTTCGACATGCAATGGCCAATTGTTTTGAAGCGTTAATAGGAGCT
  GTTTACTTGGAGGGAAGCCTGGAGGAAGCCAAGCAGTTATTTGGACGCTTGCTCTTTAATGATCCGGACC
  TGCGCGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACTGATCGACAACT
  TATTGAAACTTCTCCAGTTCTACAAAAACTTACTGAGTTTGAAGAAGCAATTGGAGTAATTTTTACTCAT
  GTTCGACTTCTGGCAAGGGCATTCACATTGAGAACTGTGGGATTTAACCATCTGACCCTAGGCCACAATC
  AGAGAATGGAATTCCTAGGTGACTCCATAATGCAACTGGTAGCCACAGAGTACTTATTCATTCATTTCCC
  AGATCATCATGAAGGACACTTAACTTTGTTGCGAAGCTCTTTGGTGAATAATAGAACTCAGGCCAAGGTA
  GCGGAGGAGCTGGGCATGCAGGAGTACGCCATAACCAACGACAAGACCAAGAGGCCTGTGGCGCTTCGCA
  CCAAGACCTTGGCGGACCTTTTGGAATCATTTATTGCAGCGCTGTACATTGATAAGGATTTGGAATATGT
  TCATACTTTCATGAATGTCTGCTTCTTTCCACGATTGAAAGAGTTCATTTTGAATCAGGATTGGAATGAC
  CCCAAATCCCAGCTTCAGCAGTGTTGCTTGACACTTAGGACAGAAGGAAAAGAGCCAGACATTCCTCTGT
  ACAAGACTCTGCAGACAGTGGGCCCATCCCATGCCCGAACCTACACTGTGGCTGTTTATTTCAAGGGAGA
  AAGAATAGGCTGTGGGAAAGGACCAAGTATTCAGCAAGCGGAAATGGGAGCAGCAATGGATGCGCTTGAA
  AAATATAATTTTCCCCAGATGGCCCATCAGAAGCGGTTCATCGAACGGAAGTACAGACAAGAGTTAAAAG
  AAATGAGGTGGGAAAGAGAGCATCAAGAGAGAGAGCCAGATGAGACTGAAGACATCAAGAAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_013235
• ORF Size: 4125 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_013235.4, NP_037367.3
• RefSeq Size: 5424
• RefSeq ORF: 4125
• Locus ID: 29102
• Domains: RIBOc, DSRM
• Gene Summary: This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.