DACH1 (NM_080759) Human Tagged ORF Clone

CAT#: RC221505

DACH1 (Myc-DDK-tagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DACH1
• Synonyms: DACH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221505 representing NM_080759
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATCTCCACGTCTG
  CTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCGGCTCCTTCCATCGGACCCCC
  GGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATCGCTTCGGCGGCGGCGGCGGCGGCCACAGTC
  ACCTCTACCGGCGGCGGCGGCGGCGGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACGGAGGCG
  GCGGTGGCGGCGGCGGCGGTGGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAGCGGCGG
  CGGCGGCGGCGGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACCGGCAGC
  AGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCT
  GCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCTCAGAATAATGA
  GTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTGGCTTCCTTCACGGTGGAGGGCTGCGAGCTGATCTGC
  CTGCCCCAGGCTTTCGACCTGTTCCTGAAGCACTTGGTGGGGGGCTTGCATACGGTCTACACCAAGCTGA
  AGCGGCTGGAGATCACGCCGGTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGGCGCCAT
  CCAGCCAGGAGTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGACTGCACC
  AACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCCCCAGAGAACTCTCACA
  TCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATTCCACCAACAGGTCTGACAGCAGCCGC
  TGCAGCAGCTGCTGCTGCTACCAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATCAAATTAGAA
  GCCATGAGCAACTATCATGCCAGTAATAACCAACATGGAGCAGACTCTGAAAACGGGGACATGAATTCAA
  GTGTCGGACTGGAACTTCCTTTTATGATGATGCCCCACCCTCTAATTCCTGTCAGCCTACCTCCAGCATC
  TGTCACCATGGCAATGAGCCAGATGAACCACCTCAGCACCATTGCAAATATGGCAGCAGCAGCACAAGTT
  CAGAGTCCCCCATCCAGAGTTGAGACATCAGTTATTAAGGAGCGTGTTCCTGATAGCCCCTCACCTGCCC
  CCTCTCTGGAGGAGGGGAGAAGGCCTGGCAGTCACCCATCATCACATCGCAGCAGCAGCGTGTCCAGCTC
  CCCTGCTCGGACTGAGAGCTCTTCTGACAGAATCCCGGTCCATCAGAATGGGTTGTCCATGAACCAGATG
  CTGATGGGCTTATCACCAAATGTACTTCCTGGGCCCAAAGAGGGAGATTTGGCCGGTCATGACATGGGAC
  ATGAGTCAAAAAGGATGCATATTGAAAAAGATGAGACCCCGCTTTCTACACCAACCGCAAGAGACAGCCT
  TGACAAACTCTCTCTAACTGGGCATGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTGTTTCCT
  GATGGACTGTCTTCCATCGAGACTCTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCCATAGATAATG
  CCAGAGCTCAAGAGAAACAGGTCCAACTGGAAAAAACTGAGCTGAAGATGGATTTTTTAAGGGAAAGAGA
  ACTAAGGGAAACACTTGAGAAGCAGTTGGCTATGGAACAAAAGAATAGAGCCATAGTTCAAAAGAGGCTA
  AAGAAGGAGAAGAAGGCAAAGAGAAAATTGCAGGAAGCACTTGAGTTTGAGACGAAACGGCGTGAACAAG
  CAGAACAGACGCTAAAACAGGCAGCTTCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGACCCCAGA
  GATAGAGGCTGACCGCAGTGGCGGCAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTG
  AAAACTACTGTCATGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221505 representing NM_080759
  Red=Cloning site Green=Tags(s)
  
  MAVPAALIPPTQLVPPQPPISTSASSSGTTTSTSSATSSPAPSIGPPASSGPTLFRPEPIASAAAAAATV
  TSTGGGGGGGGSGGGGGSSGNGGGGGGGGGGSNCNPNLAAASNGSGGGGGGISAGGGVASSTPINASTGS
  SSSSSSSSSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVENTPQNNECKMVDLRGAKVASFTVEGCELIC
  LPQAFDLFLKHLVGGLHTVYTKLKRLEITPVVCNVEQVRILRGLGAIQPGVNRCKLISRKDFETLYNDCT
  NASSRPGRPPKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIKLE
  AMSNYHASNNQHGADSENGDMNSSVGLELPFMMMPHPLIPVSLPPASVTMAMSQMNHLSTIANMAAAAQV
  QSPPSRVETSVIKERVPDSPSPAPSLEEGRRPGSHPSSHRSSSVSSSPARTESSSDRIPVHQNGLSMNQM
  LMGLSPNVLPGPKEGDLAGHDMGHESKRMHIEKDETPLSTPTARDSLDKLSLTGHGQPLPPGFPSPFLFP
  DGLSSIETLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRL
  KKEKKAKRKLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAERTIQDGRLYL
  KTTVMY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080759
• ORF Size: 2118 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080759.1, NM_080759.2, NM_080759.3, NM_080759.4, NM_080759.5, NP_542937.1, NP_542937.3
• RefSeq Size: 5240 bp
• RefSeq ORF: 2121 bp
• Locus ID: 1602
• Cytogenetics: 13q21.33
• Domains: Ski_Sno
• Protein Families: Transcription Factors
• MW: 73 kDa
• Gene Summary: 'This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'