DACH1 (NM_080759) Human Untagged Clone

CAT#: SC327980

DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1) transcript variant 1


  "NM_080759" in other vectors (7)

Reconstitution Protocol

USD 1,440.00

2 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DACH1
Synonyms DACH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_080759, the custom clone sequence may differ by one or more nucleotides


ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATCTCCACGTCTG
CTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCGGCTCCTTCCATCGGACCCCC
GGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATCGCTTCGGCGGCGGCGGCGGCGGCCACAGTC
ACCTCTACCGGCGGCGGCGGCGGCGGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACGGAGGCG
GCGGTGGCGGCGGCGGCGGTGGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAGCGGCGG
CGGCGGCGGCGGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACCGGCAGC
AGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCT
GCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCTCAGAATAATGA
GTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTGGCTTCCTTCACGGTGGAGGGCTGCGAGCTGATCTGC
CTGCCCCAGGCTTTCGACCTGTTCCTGAAGCACTTGGTGGGGGGCTTGCATACGGTCTACACCAAGCTGA
AGCGGCTGGAGATCACGCCGGTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGGCGCCAT
CCAGCCAGGAGTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGACTGCACC
AACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCCCCAGAGAACTCTCACA
TCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATTCCACCAACAGGTCTGACAGCAGCCGC
TGCAGCAGCTGCTGCTGCTACCAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATCAAATTAGAA
GCCATGAGCAACTATCATGCCAGTAATAACCAACATGGAGCAGACTCTGAAAACGGGGACATGAATTCAA
GTGTCGGACTGGAACTTCCTTTTATGATGATGCCCCACCCTCTAATTCCTGTCAGCCTACCTCCAGCATC
TGTCACCATGGCAATGAGCCAGATGAACCACCTCAGCACCATTGCAAATATGGCAGCAGCAGCACAAGTT
CAGAGTCCCCCATCCAGAGTTGAGACATCAGTTATTAAGGAGCGTGTTCCTGATAGCCCCTCACCTGCCC
CCTCTCTGGAGGAGGGGAGAAGGCCTGGCAGTCACCCATCATCACATCGCAGCAGCAGCGTGTCCAGCTC
CCCTGCTCGGACTGAGAGCTCTTCTGACAGAATCCCGGTCCATCAGAATGGGTTGTCCATGAACCAGATG
CTGATGGGCTTATCACCAAATGTACTTCCTGGGCCCAAAGAGGGAGATTTGGCCGGTCATGACATGGGAC
ATGAGTCAAAAAGGATGCATATTGAAAAAGATGAGACCCCGCTTTCTACACCAACCGCAAGAGACAGCCT
TGACAAACTCTCTCTAACTGGGCATGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTGTTTCCT
GATGGACTGTCTTCCATCGAGACTCTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCCATAGATAATG
CCAGAGCTCAAGAGAAACAGGTCCAACTGGAAAAAACTGAGCTGAAGATGGATTTTTTAAGGGAAAGAGA
ACTAAGGGAAACACTTGAGAAGCAGTTGGCTATGGAACAAAAGAATAGAGCCATAGTTCAAAAGAGGCTA
AAGAAGGAGAAGAAGGCAAAGAGAAAATTGCAGGAAGCACTTGAGTTTGAGACGAAACGGCGTGAACAAG
CAGAACAGACGCTAAAACAGGCAGCTTCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGACCCCAGA
GATAGAGGCTGACCGCAGTGGCGGCAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTG
AAAACTACTGTCATGTACTGA


Restriction Sites SgfI-MluI     
ACCN NM_080759
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080759.5, NP_542937.3
RefSeq Size 5240 bp
RefSeq ORF 2121 bp
Locus ID 1602
Cytogenetics 13q21.33
Domains Ski_Sno
Protein Families Transcription Factors
Gene Summary 'This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'
Transcript Variant: This variant (1) is the predominant transcript and it encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to remove two codons that are not included in the GRCh38 reference genome allele.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.