DACH1 (NM_080759) Human Untagged Clone

CAT#: SC120348

DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DACH1
• Synonyms: DACH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_080759, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATCTCCACGTCTG
  CTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCGGCTCCTTCCATCGGACCCCC
  GGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATCGCTTCGGCGGCGGCGGCGGCGGCCACAGTC
  ACCTCTACCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACG
  GAGGCGGCGGTGGCGGCGGCGGCGGTGGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAG
  CGGCGGCGGCGGCGGCGGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACC
  GGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGCAGTAGCAGCA
  GCAGCTGCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCTCAGAA
  TAATGAGTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTGGCTTCCTTCACGGTGGAGGGCTGCGAGCTG
  ATCTGCCTGCCCCAGGCTTTCGACCTGTTCCTGAAGCACTTGGTGGGGGGCTTGCATACGGTCTACACCA
  AGCTGAAGCGGCTGGAGATCACGCCGGTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGG
  CGCCATCCAGCCAGGAGTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGAC
  TGCACCAACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCCCCAGAGAACT
  CTCACATCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATTCCACCAACAGGTCTGACAGC
  AGCCGCTGCAGCAGCTGCTGCTGCTACCAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATCAAA
  TTAGAAGCCATGAGCAACTATCATGCCAGTAATAACCAACATGGAGCAGACTCTGAAAACGGGGACATGA
  ATTCAAGTGTCGGACTGGAACTTCCTTTTATGATGATGCCCCACCCTCTAATTCCTGTCAGCCTACCTCC
  AGCATCTGTCACCATGGCAATGAGCCAGATGAACCACCTCAGCACCATTGCAAATATGGCAGCAGCAGCA
  CAAGTTCAGAGTCCCCCATCCAGAGTTGAGACATCAGTTATTAAGGAGCGTGTTCCTGATAGCCCCTCAC
  CTGCCCCCTCTCTGGAGGAGGGGAGAAGGCCTGGCAGTCACCCATCATCACATCGCAGCAGCAGCGTGTC
  CAGCTCCCCTGCTCGGACTGAGAGCTCTTCTGACAGAATCCCGGTCCATCAGAATGGGTTGTCCATGAAC
  CAGATGCTGATGGGCTTATCACCAAATGTACTTCCTGGGCCCAAAGAGGGAGATTTGGCCGGTCATGACA
  TGGGACATGAGTCAAAAAGGATGCATATTGAAAAAGATGAGACCCCGCTTTCTACACCAACCGCAAGAGA
  CAGCCTTGACAAACTCTCTCTAACTGGGCATGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTG
  TTTCCTGATGGACTGTCTTCCATCGAGACTCTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCCATAG
  ATAATGCCAGAGCTCAAGAGAAACAGGTCCAACTGGAAAAAACTGAGCTGAAGATGGATTTTTTAAGGGA
  AAGAGAACTAAGGGAAACACTTGAGAAGCAGTTGGCTATGGAACAAAAGAATAGAGCCATAGTTCAAAAG
  AGGCTAAAGAAGGAGAAGAAGGCAAAGAGAAAATTGCAGGAAGCACTTGAGTTTGAGACGAAACGGCGTG
  AACAAGCAGAACAGACGCTAAAACAGGCAGCTTCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGAC
  CCCAGAGATAGAGGCTGACCGCAGTGGCGGCAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTG
  TATTTGAAAACTACTGTCATGTACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080759 unedited
  GAATTTGTAATACGACCTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGAGGACTC
  GCATTTCGACTTGCGGGACACTTTTGTGCGTTCCTCTCCAGAGCGCCTCTCGTGCTCGCC
  CCTCTTGCGCTCGCTCTTTATTACCTTCACCTCCTTTTCTCCCCCTTCTCTCCCTTTCTC
  CTTCTCGTTCTCTCCCGGAGTTGTTGTTGCCCCCCTCGCTCCTTCTCCCCCCTTTTTTCC
  CCTTCCCCTCCCGGGGGTGTGTGGCAACTTTTCCTCTCGCTTCTCCTCCGTCTGTTTCCC
  CTTATATGTGACCATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCC
  TCAACCCCCAATCTCCACGTCTGCTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGC
  GACTTCGTCTCCGGCTCCTTCCATCGGACCCCCGGCGTCCTCTGGGCCAACTCTGTTCCG
  CCCGGAGCCCATCGCTTCGGCGGCGGCGGCGGCGGCCACAGTCACCTCTACCGGCGGCGG
  CGGCGGCGGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACGGAGGCGGCGGTGG
  CGGCGGCGGCGGGTGCANCAACTGCAACCCCAACCTGNCGGCCGCGAGCAACGGCAGCGG
  CGGCGGCGGCGGCGGCATCACCGCTGGCGGCNGGCGTCGCTTCCAGCACCCCCATCAACG
  CCAGCACCGGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCA
  GCAGCAGCAGTAGCACACAGCTTGGGGGCCCCCTCCCCGGAAACCCCTGTACTTCACCCC
  CGTCCCAGTGGAAAACACCCTCAGATTATGGTGCCAATGGTGGATCTGGGAGGGGCCCAA
  GTTGGTTCCTTACGGGGGGAGGCTGGAAGCTGATTTGCCTGCCCAAGCTTTGAACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_080759 unedited
  GTACCGCGNGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTAGAATACTTAAA
  CTTTTAAAGAACATTAATACACAAAATTCAGGAAGTTCCCTTAAAAGGACTTTATTTTTT
  TCTGAACTTTCCCATGACGAATGTCTGACTGCAAAGTTCTTTTCTATAACATGGATTTCT
  TCAACAGGAAAGATTCAGTACATGACAGTAGTTTTCAAATACAGTCTTCCATCTTGTATT
  GTCCTTTCAGCATCTGTTCTGCCGCCACTGCGGTCAGCCTCTATCTCTGGGGTCAGAGAG
  TCATTTAAGACCCTGAGACTATCTGTTGAAGCTGCCTGTTTTAGCGTCTGTTCTGCTTGT
  TCACGCCGTTTCGTCTCAAACTCAAGTGCTTCCTGCAATTTTCTCTTTGCCTTCTTCTCC
  TTCTTTAGCCTCTTTTGAACTATGGCTCTATTCTTTTGTTCCATAGCCAACTGCTTCTCA
  AGTGTTTCCCTTAGTTCTCTTTCCCTTAAAAAATCCATCTTCAGCTCAGTTTTTTCCAGT
  TGGACCTGTTTCTCTTGAGCTCTGGCATTATCTATGGCAACTTTCAACAGCCCCTGTATG
  TTAGTCAGAAGAGTCTCGATGGAAGACAGTCCATCAGGAAACAGAAAAGGAGATGGAAAA
  CCTGGAGGCAGTGGTTGTCCATGCCCAGTTAGAGAGAGTTTGTCAAGGCTGTCTCTTGCG
  GTTGGTGTANAAAAGCGGGTCTCATCTTTTTCATATGCATCCTTTTTGACTCATGTCCCA
  TGTCATGACCGGCCAAATCTNCCTCTTTGGGCCCAGNAAGTACAATTGGTGATTAGCCCA
  TCAGCATCTGGTTCATGGACAACCCATTCTGATGGACGGGGATNCTGTCAGAGAGCTCTC
  ANTCCGAGCAGGGANCTTGACCCCTGCTGCTGCAATTGATAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_080759
• Insert Size: 2680 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080759.3, NP_542937.1
• RefSeq Size: 5246 bp
• RefSeq ORF: 2121 bp
• Locus ID: 1602
• Cytogenetics: 13q21.33
• Domains: Ski_Sno
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (1) is the predominant transcript and it encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to remove two codons that are not included in the GRCh38 reference genome allele.