PFKFB4 (NM_004567) Human Tagged ORF Clone

CAT#: RG201573

PFKFB4 (GFP-tagged) - Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PFKFB4
• Synonyms: 6-phosphofructo-2-kinase; bifunctional enzyme with kinase and biphosphatase activities; fructose-2,6-biphosphatase-4; fructose-2,6-biphosphatase 4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201573 representing NM_004567
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCCCACGGGAATTGACACAGAACCCCCTGAAGAAGATCTGGATGCCATACAGCAATGGGCGGC
  CCGCTCTGCACGCTTGCCAGCGCGGTGTGTGCATGACCAACTGCCCAACTCTCATTGTCATGGTGGGCCT
  GCCCGCCAGGGGCAAGACCTACATCTCCAAGAAGCTGACTCGATACCTGAACTGGATTGGTGTGCCCACT
  CGGGAGTTCAATGTTGGCCAGTATCGCCGGGACGTGGTCAAGACCTACAAATCTTTTGAATTTTTTCTCC
  CCGACAATGAAGAGGGCCTGAAAATCAGGAAGCAGTGTGCCCTGGCAGCCCTCCGTGACGTCCGGCGGTT
  CCTTAGTGAGGAGGGGGGACATGTGGCGGTTTTTGATGCCACAAACACCACCCGAGAACGGAGAGCGACC
  ATCTTTAATTTTGGAGAACAGAATGGCTACAAGACCTTTTTTGTCGAGTCCATCTGTGTGGATCCTGAGG
  TCATAGCTGCCAACATCGTGCAAGTGAAACTGGGCAGCCCTGACTATGTCAACCGCGACAGTGATGAGGC
  TACGGAGGACTTCATGAGGCGCATTGAGTGCTATGAGAACTCCTACGAGTCGCTAGATGAGGACCTGGAT
  AGGGACCTGTCCTATATCAAGATCATGGATGTGGGCCAGAGCTACGTGGTGAACCGTGTGGCTGACCACA
  TCCAGAGCCGCATCGTATATTACCTCATGAACATCCACGTGACCCCCCGCTCCATCTACCTCTGCCGGCA
  CGGGGAGAGCGAGCTCAACCTCAAGGGCCGGATTGGCGGGGACCCAGGACTGTCCCCTCGGGGCAGGGAG
  TTTGCCAAGAGTCTAGCCCAGTTCATCAGTGACCAAAATATCAAGGATCTGAAGGTCTGGACAAGCCAGA
  TGAAGAGGACAATCCAGACGGCTGAGGCACTGGGTGTGCCCTATGAACAGTGGAAGGTCCTCAACGAGAT
  CGATGCGGGCGTCTGTGAGGAAATGACCTACGAGGAAATTCAGGATAATTATCCACTGGAGTTCGCCCTG
  CGGGACCAGGACAAGTACCGGTACCGGTACCCTAAAGGGGAGTCCTACGAGGACCTGGTCCAGAGACTGG
  AGCCTGTCATCATGGAGCTGGAGAGGCAAGAGAATGTGCTGGTCATCTGCCACCAGGCTGTGATGCGCTG
  CCTGCTGGCCTACTTCCTCGACAAGGCAGCAGAACAGCTGCCCTACCTCAAGTGTCCGCTGCACACAGTC
  CTGAAGCTGACTCCTGTGGCATATGGTTGTAAAGTGGAGTCCATATTCCTGAACGTGGCTGCTGTGAACA
  CGCACCGGGACAGGCCTCAGAACGTGGACATCTCAAGACCTCCAGAGGAAGCCCTTGTCACGGTGCCTGC
  TCACCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201573 representing NM_004567
  Red=Cloning site Green=Tags(s)
  
  MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPT
  REFNVGQYRRDVVKTYKSFEFFLPDNEEGLKIRKQCALAALRDVRRFLSEEGGHVAVFDATNTTRERRAT
  IFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLD
  RDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGRE
  FAKSLAQFISDQNIKDLKVWTSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEEMTYEEIQDNYPLEFAL
  RDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTV
  LKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPPEEALVTVPAHQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004567
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004567.2, NP_004558.1
• RefSeq Size: 3503 bp
• RefSeq ORF: 1410 bp
• Locus ID: 5210
• Cytogenetics: 3p21.31
• Domains: PGAM, 6PF2K
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism
• Gene Summary: 'The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]'