PFKFB4 (NM_004567) Human Untagged Clone

CAT#: SC323929

PFKFB4 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PFKFB4
• Synonyms: 6-phosphofructo-2-kinase; bifunctional enzyme with kinase and biphosphatase activities; fructose-2,6-biphosphatase-4; fructose-2,6-biphosphatase 4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004567.2 CTCATCCCGGCCCCGGGATGGCGTCCCCACGGGAATTGACACAGAACCCCCTGAAGAAGA
  TCTGGATGCCATACAGCAATGGGCGGCCCGCTCTGCACGCTTGCCAGCGCGGTGTGTGCA
  TGACCAACTGCCCAACTCTCATTGTCATGGTGGGCCTGCCCGCCAGGGGCAAGACCTACA
  TCTCCAAGAAGCTGACTCGATACCTGAACTGGATTGGTGTGCCCACTCGGGAGTTCAATG
  TTGGCCAGTATCGCCGGGACGTGGTCAAGACCTACAAATCTTTTGAATTTTTTCTCCCCG
  ACAATGAAGAGGGCCTGAAAATCAGGAAGCAGTGTGCCCTGGCAGCCCTCCGTGACGTCC
  GGCGGTTCCTTAGTGAGGAGGGGGGACATGTGGCGGTTTTTGATGCCACAAACACCACCC
  GAGAACGGAGAGCGACCATCTTTAATTTTGGAGAACAGAATGGCTACAAGACCTTTTTTG
  TCGAGTCCATCTGTGTGGATCCTGAGGTCATAGCTGCCAACATCGTGCAAGTGAAACTGG
  GCAGCCCTGACTATGTCAACCGCGACAGTGATGAGGCTACGGAGGACTTCATGAGGCGCA
  TTGAGTGCTATGAGAACTCCTACGAGTCGCTAGATGAGGACCTGGATAGGGACCTGTCCT
  ATATCAAGATCATGGATGTGGGCCAGAGCTACGTGGTGAACCGTGTGGCTGACCACATCC
  AGAGCCGCATCGTATATTACCTCATGAACATCCACGTGACCCCCCGCTCCATCTACCTCT
  GCCGGCACGGGGAGAGCGAGCTCAACCTCAAGGGCCGGATTGGCGGGGACCCAGGACTGT
  CCCCTCGGGGCAGGGAGTTTGCCAAGAGTCTAGCCCAGTTCATCAGTGACCAAAATATCA
  AGGATCTGAAGGTCTGGACAAGCCAGATGAAGAGGACAATCCAGACGGCTGAGGCACTGG
  GTGTGCCCTATGAACAGTGGAAGGTCCTCAACGAGATCGATGCGGGCGTCTGTGAGGAAA
  TGACCTACGAGGAAATTCAGGATAATTATCCACTGGAGTTCGCCCTGCGGGACCAGGACA
  AGTACCGGTACCGGTACCCTAAAGGGGAGTCCTACGAGGACCTGGTCCAGAGACTGGAGC
  CTGTCATCATGGAGCTGGAGAGGCAAGAGAATGTGCTGGTCATCTGCCACCAGGCTGTGA
  TGCGCTGCCTGCTGGCCTACTTCCTCGACAAGGCAGCAGAACAGCTGCCCTACCTCAAGT
  GTCCGCTGCACACAGTCCTGAAGCTGACTCCTGTGGCATATGGTTGTAAAGTGGAGTCCA
  TATTCCTGAACGTGGCTGCTGTGAACACGCACCGGGACAGGCCTCAGAACGTGGACATCT
  CAAGACCTCCAGAGGAAGCCCTTGTCACGGTGCCTGCTCACCAGTGACCATGTTCATCCA
  CTGTGACCACTAGGCAGGCACTGCTCTCTGCAGAGGGGGTCATTCCAGGCCCTCCAGTGT
  GTGTGATAGTCACCATGCCATGCAGGGATATTCTTGAAGCCACACATGGCTGGCGGAACC
  CAGAGCCCCCACCCCAGCCCACCTGGCTCTTTGTTGACAGTCGGCGACAAGGTTGTGCGT
  GGCTCCTGACCTGCTGCTAAGAGTCACTTGACCAGACTGCATCTGCATGGGCTGCGCGGA
  GGTTGCCCAGCCCCAGTTTCTTCCGGCGCAGCTCTTAGGTGTTCACTCTCGCCAGCTCAG
  TTGGCTTTGTGAAGTGTGAAACCCTACAATGTGAAAGGAAAGTGCTTGCTGTGATGTTCC
  TACTGTGGCCCAGCTGCCCAGCATGGACCTGGTGACTCTCCACAGGGCCTCTACCATCCT
  CTCTGTGGCCACTTCCTGAGCCAGAGGCCAGGTCTTCATGGGGCCCTGAGCTTCTGCTGC
  CTCTGGTGAGAGGGAGAGCCCTTCCCATCCTTACCCACCAGGAACTAGAGCCCCAACCAC
  AGCAGATGCTTCCTGGGCAGCCACTGGCCAGGCCGTTGTATCCATGTCACCCTTAGTTGT
  GGGCATTCATGAAAGCAATGCGCTTGCTTCAGCACATTGGGATGCACAGAAACGTGAGGG
  CAGGGGGGCAGTTGTGGTCGCCCCTCCCCAGTTGTGCATGTACCCGGCTACTTAGGTATG
  CTTAGGGGAGCTGGTGGCCAGGGGTGCCCCTTCAGGCCTGCTCTCACAGTGAAACCACTT
  CAAGACAGGGCAGGAAGTTACCTTCCTGGTGCACACACCGAAGTGCTCGAGGGAGAGGCT
  GCCGCCCCCTCTCCCCTTCCCTGGCTGTGGACACTGGCAGCCAATGGGGAGGCGGCCTCT
  CCCCTGTTGCTGCCCGTGTCCACACAGGAATGTACACTGGTGGCAGAAACATGCATTTGC
  CATAAATAATTCAGAAACACTTGTATGGTCCTGTAAAACTTCTCTGGTGTTTGTTGACTT
  CTGCCTGTGGGTCTGAGGAAGGGAGAAGGCAGAACCCATAGGCAGTCCCTGGGCAAGGGA
  GGATTTTCCCGGCCGCAGCACAGCCTTCCATTCATGTGCACGTGAGTCTGGTGAAAATGG
  CCTTGTGAAGTCCAGTGTCTGTCATGCCCCTGAGTTCTTTTGGTCCCAGAGAGGCAGCCT
  GGGCAGTCTGTTTACAGCTGGCAAACAGACTGGCTGGCAAGTTGTGGGGCTGGGTCAGAA
  AGTGTCTGGACTTGTAATCGGGTGCCCTGCTGTCCACAGGTGGACTCTGGGGAAGCCAGG
  CCCCTGGATCCCAAGTCCTTTGTGTCCGGAGCCTTTCCTGGCCTGTGCCTGGAGGGTAGC
  ACATCTTTCCAGCCTCCCAGGGCTTTGCAGCCTGCAGATGCCACCTGTCCCTGAGAGAAG
  TTAGCGTCCCTGGCCCTGGCCGGTGGTCTCTCATCCTTGTGTGCTGCTCTGCTAAGAGAT
  GTCCAAGGCGGAGCCGGGGCAAGATCCTTCCAGACTCATCTGTCAGAGCCCCAAGCCCTT
  TAGACCCAGAGCCCAAGGACCATGCCTTTGGGACATTAGGACTGCAGCCTTTGCTTCTGT
  GTATTTTGGAGTTTTGGTGACTTTTGTCACCTGGACACACTCATTTGTTAGCCATAGTGG
  GTTCCCTTGGTCAGCAACAGTGCATGTACCTCTGGATGTCATCTGAGGTGAGACCACCGA
  GGCCTTTTCTCTCTGTGTACAGAGGGGAGTTAGGAGTTGCTGGACTGGATGCATTACGAG
  GACTGGGGACAGGGTAGAGGGACATCCAGGGATCAGGGCATGAGTGGGGGCAACCCCCCG
  GCCTCTGCCCTGGCATGGTCTCCGCATGGGCTGAGGTGTAGCTGATTGGCTGCCACATTT
  CGGCCATGCTGGCTGGCGTGCCCATGTTGCAGATATTTTCCCGAGTTCCCCAGAATGGAT
  GGTATTGAATCTCAGCCACATGCAACACTGTGTCCAGCATTCTTTGCAATAAATACTTTT
  TAAAAAATAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004567
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004567.2, NP_004558.1
• RefSeq Size: 3503 bp
• RefSeq ORF: 1410 bp
• Locus ID: 5210
• Cytogenetics: 3p21.31
• Domains: PGAM, 6PF2K
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism
• Gene Summary: 'The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (2) lacks an alternate coding exon in the 5' end compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.