PFKFB4 (NM_004567) Human Untagged Clone

CAT#: SC110972

PFKFB4 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4)


  "NM_004567" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PFKFB4
Synonyms 6-phosphofructo-2-kinase; bifunctional enzyme with kinase and biphosphatase activities; fructose-2,6-biphosphatase-4; fructose-2,6-biphosphatase 4
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC110972 sequence for NM_004567 edited (data generated by NextGen Sequencing)
ATGGCGTCCCCACGGGAATTGACACAGAACCCCCTGAAGAAGATCTGGATGCCATACAGC
AATGGGCGGCCCGCTCTGCACGCTTGCCAGCGCGGTGTGTGCATGACCAACTGCCCAACT
CTCATTGTCATGGTGGGCCTGCCCGCCAGGGGCAAGACCTACATCTCCAAGAAGCTGACT
CGATACCTGAACTGGATTGGTGTGCCCACTCGGGAGTTCAATGTTGGCCAGTATCGCCGG
GACGTGGTCAAGACCTACAAATCTTTTGAATTTTTTCTCCCCGACAATGAAGAGGGCCTG
AAAATCAGGAAGCAGTGTGCCCTGGCAGCCCTCCGTGACGTCCGGCGGTTCCTTAGTGAG
GAGGGGGGACATGTGGCGGTTTTTGATGCCACAAACACCACCCGAGAACGGAGAGCGACC
ATCTTTAATTTTGGAGAACAGAATGGCTACAAGACCTTTTTTGTCGAGTCCATCTGTGTG
GATCCTGAGGTCATAGCTGCCAACATCGTGCAAGTGAAACTGGGCAGCCCTGACTATGTC
AACCGCGACAGTGATGAGGCTACGGAGGACTTCATGAGGCGCATTGAGTGCTATGAGAAC
TCCTACGAGTCGCTAGATGAGGACCTGGATAGGGACCTGTCCTATATCAAGATCATGGAT
GTGGGCCAGAGCTACGTGGTGAACCGTGTGGCTGACCACATCCAGAGCCGCATCGTATAT
TACCTCATGAACATCCACGTGACCCCCCGCTCCATCTACCTCTGCCGGCACGGGGAGAGC
GAGCTCAACCTCAAGGGCCGGATTGGCGGGGACCCAGGACTGTCCCCTCGGGGCAGGGAG
TTTGCCAAGAGTCTAGCCCAGTTCATCAGTGACCAAAATATCAAGGATCTGAAGGTCTGG
ACAAGCCAGATGAAGAGGACAATCCAGACGGCTGAGGCACTGGGTGTGCCCTATGAACAG
TGGAAGGTCCTCAACGAGATCGATGCGGGCGTCTGTGAGGAAATGACCTACGAGGAAATT
CAGGATAATTATCCACTGGAGTTCGCCCTGCGGGACCAGGACAAGTACCGGTACCGGTAC
CCTAAAGGGGAGTCCTACGAGGACCTGGTCCAGAGACTGGAGCCTGTCATCATGGAGCTG
GAGAGGCAAGAGAATGTGCTGGTCATCTGCCACCAGGCTGTGATGCGCTGCCTGCTGGCC
TACTTCCTCGACAAGGCAGCAGAACAGCTGCCCTACCTCAAGTGTCCGCTGCACACAGTC
CTGAAGCTGACTCCTGTGGCATATGGTTGTAAAGTGGAGTCCATATTCCTGAACGTGGCT
GCTGTGAACACGCACCGGGACAGGCCTCAGAACGTGGACATCTCAAGACCTCCAGAGGAA
GCCCTTGTCACGGTGCCTGCTCACCAGTGA

Clone variation with respect to NM_004567.2
>OriGene 5' read for NM_004567 unedited
GCGATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGAGGTGGCCCGGCT
GCTGGGTTGGAAGCGGCGCAGTCCGACTCATCCCGGCCCCGGGATGGCGTCCCCACGGGN
AATTGACACAGAACCCCCTGAAGAAGATCTGGATGCCATACAGCAATGGGCGGCCCGGTC
TGCACGCTTGCCAGCGCGGTGTGTGCATGACCAACTGCCCAACTCTCATTGTCATGGTGG
GCCTGCCCGCCAGGGGCAAGACCTACATCTCCAAGAGGCTGACTCGATACCTGAACTGGA
TTGGTGTGCCCACTCGGGAGTTCAATGTTGGCCAGTATCGCCGGGACGTGGTCAAGACCT
ACAAATCTTTTGAATTTTTTCTCCCCGACAATGAAGAGGGCCTGAAAATCAGGAAGCAGT
GTGCCCTGGCAGCCCTCCGTGACGTCCGGCGGTTCCTTAGTGAGGAGGGGGGACATGTGG
CGGTTTTTGATGCCACAAACACCACCCGAGAACGGAGAGCGACCATCTTTAATTTTGGAG
AACAGAATGGCTACAAGACCTTTTTTGTCGAGTCCATCTGTGTGGATCCTGAGGTCATAG
CTGCCAACATCGTGCAAGTGAAACTGGGCAGCCCTGACTATGTCAACCGCGACAGTGATG
AGGCTACCGAGGACTTCATGAGGCGCATTGAGTGCTATGAGAACTCCTACCAGTCGCTAG
ATGAGGACCCTGATAGGGACCTGTCCTATATAAGATCATGGATGTGGGCCCAGAGCTACG
TGGTGAACCGTGTGGCTGACCCCATCCCGACCCGAACGTATATTACCTCATGAACATTCC
ACGTGACCCCCGGTCCATCTACCTTGGNCGGAACGGGGAGAGCGAGGCTAACCTCCAGGC
GCGCGATGGTGGGGACCCAGACTGTCCCCTCGGGCAGGTAGTTTGCCAGAGTTTC
>OriGene 3' read for NM_004567 unedited
AGCTATGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAT
TTTTTAAAAAGTATTTATTGCAAAGAATGCTGGCACACAGTGTTGCATGTGGCTGAGATT
CAATACCATCCATTCTGGGGAACTCGGGAAAATATCTGCAACATGGGCACGCCAGCCAGC
ATGGCCGAAATGTGGCAGCCAATCAGCTACACCTCAGCCCATGCGGAGACCATGCCAGGG
CAGAGGCCGGGGGGTTGCCCCCACTCATGCCCTGATCCCTGGATGTCCCTCTACCCTGTC
CCCAGTCCTCGTAATGCATCCAGTCCAGCAACTCCTAACTCCCCTCTGTACACAGAGAGA
AAAGGCCTCGGCGGTCTCACCTCAGATGACATCCAGAGGTACATGCACTGTTGCTGACCA
AGGGAACCCACTATGGCTAACAAATGAGTGTGTCCAGGTGACAAAAGTCACCAAAACTCC
AAAATACACAGAAGCAAAGGCTGCACTCCTAATGTCCCAAAGGCATGGTCCTTGGGCTCT
GGGTCTAAAGGGCTTGGGGCTCTCACACAGAATCTGGAGGATCTTGCCCCCGGTCCCCCT
GGACATTTCTTACCGAGCCCCCCCAGGCTGAGACACACCGACCCGGGCACGGGCCCTATC
TTCAGTCGCGCTCCGGGGAGCTCTCGGGACTCGCCTAACCCGGGAACCTGGAAATATCGC
CCTCCCTTTCGACATTGAACATCACGGGCGCGCGCGCCAGAGGCCACTCCCTCCATGGCA
CCCGCCTACACGCCTCGTCGCCCCTTTCACCCATACCGACCCTCATCAACCGCCCCAACC
CTACAATATTCACTCTACATCACCACACAACACCATTGTCACGTAACACCCGCACACCCC
TCAGATTCCGCCGCCGGCCGGTCCCTGCTCCTTGGCCAGTACNAGCATCTAGCCGTACTC
CACCCGCGTC
Restriction Sites ECoRI-NOT     
ACCN NM_004567
Insert Size 3630 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004567.2, NP_004558.1
RefSeq Size 3503 bp
RefSeq ORF 1410 bp
Locus ID 5210
Cytogenetics 3p21.31
Domains PGAM, 6PF2K
Protein Families Druggable Genome
Protein Pathways Fructose and mannose metabolism
Gene Summary 'The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]'
Transcript Variant: This variant (2) lacks an alternate coding exon in the 5' end compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.

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