PFKFB4 (NM_004567) Human Untagged Clone

CAT#: SC110972

PFKFB4 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PFKFB4
• Synonyms: 6-phosphofructo-2-kinase; bifunctional enzyme with kinase and biphosphatase activities; fructose-2,6-biphosphatase-4; fructose-2,6-biphosphatase 4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110972 sequence for NM_004567 edited (data generated by NextGen Sequencing)
  ATGGCGTCCCCACGGGAATTGACACAGAACCCCCTGAAGAAGATCTGGATGCCATACAGC
  AATGGGCGGCCCGCTCTGCACGCTTGCCAGCGCGGTGTGTGCATGACCAACTGCCCAACT
  CTCATTGTCATGGTGGGCCTGCCCGCCAGGGGCAAGACCTACATCTCCAAGAAGCTGACT
  CGATACCTGAACTGGATTGGTGTGCCCACTCGGGAGTTCAATGTTGGCCAGTATCGCCGG
  GACGTGGTCAAGACCTACAAATCTTTTGAATTTTTTCTCCCCGACAATGAAGAGGGCCTG
  AAAATCAGGAAGCAGTGTGCCCTGGCAGCCCTCCGTGACGTCCGGCGGTTCCTTAGTGAG
  GAGGGGGGACATGTGGCGGTTTTTGATGCCACAAACACCACCCGAGAACGGAGAGCGACC
  ATCTTTAATTTTGGAGAACAGAATGGCTACAAGACCTTTTTTGTCGAGTCCATCTGTGTG
  GATCCTGAGGTCATAGCTGCCAACATCGTGCAAGTGAAACTGGGCAGCCCTGACTATGTC
  AACCGCGACAGTGATGAGGCTACGGAGGACTTCATGAGGCGCATTGAGTGCTATGAGAAC
  TCCTACGAGTCGCTAGATGAGGACCTGGATAGGGACCTGTCCTATATCAAGATCATGGAT
  GTGGGCCAGAGCTACGTGGTGAACCGTGTGGCTGACCACATCCAGAGCCGCATCGTATAT
  TACCTCATGAACATCCACGTGACCCCCCGCTCCATCTACCTCTGCCGGCACGGGGAGAGC
  GAGCTCAACCTCAAGGGCCGGATTGGCGGGGACCCAGGACTGTCCCCTCGGGGCAGGGAG
  TTTGCCAAGAGTCTAGCCCAGTTCATCAGTGACCAAAATATCAAGGATCTGAAGGTCTGG
  ACAAGCCAGATGAAGAGGACAATCCAGACGGCTGAGGCACTGGGTGTGCCCTATGAACAG
  TGGAAGGTCCTCAACGAGATCGATGCGGGCGTCTGTGAGGAAATGACCTACGAGGAAATT
  CAGGATAATTATCCACTGGAGTTCGCCCTGCGGGACCAGGACAAGTACCGGTACCGGTAC
  CCTAAAGGGGAGTCCTACGAGGACCTGGTCCAGAGACTGGAGCCTGTCATCATGGAGCTG
  GAGAGGCAAGAGAATGTGCTGGTCATCTGCCACCAGGCTGTGATGCGCTGCCTGCTGGCC
  TACTTCCTCGACAAGGCAGCAGAACAGCTGCCCTACCTCAAGTGTCCGCTGCACACAGTC
  CTGAAGCTGACTCCTGTGGCATATGGTTGTAAAGTGGAGTCCATATTCCTGAACGTGGCT
  GCTGTGAACACGCACCGGGACAGGCCTCAGAACGTGGACATCTCAAGACCTCCAGAGGAA
  GCCCTTGTCACGGTGCCTGCTCACCAGTGA
  
  Clone variation with respect to NM_004567.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004567 unedited
  GCGATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGAGGTGGCCCGGCT
  GCTGGGTTGGAAGCGGCGCAGTCCGACTCATCCCGGCCCCGGGATGGCGTCCCCACGGGN
  AATTGACACAGAACCCCCTGAAGAAGATCTGGATGCCATACAGCAATGGGCGGCCCGGTC
  TGCACGCTTGCCAGCGCGGTGTGTGCATGACCAACTGCCCAACTCTCATTGTCATGGTGG
  GCCTGCCCGCCAGGGGCAAGACCTACATCTCCAAGAGGCTGACTCGATACCTGAACTGGA
  TTGGTGTGCCCACTCGGGAGTTCAATGTTGGCCAGTATCGCCGGGACGTGGTCAAGACCT
  ACAAATCTTTTGAATTTTTTCTCCCCGACAATGAAGAGGGCCTGAAAATCAGGAAGCAGT
  GTGCCCTGGCAGCCCTCCGTGACGTCCGGCGGTTCCTTAGTGAGGAGGGGGGACATGTGG
  CGGTTTTTGATGCCACAAACACCACCCGAGAACGGAGAGCGACCATCTTTAATTTTGGAG
  AACAGAATGGCTACAAGACCTTTTTTGTCGAGTCCATCTGTGTGGATCCTGAGGTCATAG
  CTGCCAACATCGTGCAAGTGAAACTGGGCAGCCCTGACTATGTCAACCGCGACAGTGATG
  AGGCTACCGAGGACTTCATGAGGCGCATTGAGTGCTATGAGAACTCCTACCAGTCGCTAG
  ATGAGGACCCTGATAGGGACCTGTCCTATATAAGATCATGGATGTGGGCCCAGAGCTACG
  TGGTGAACCGTGTGGCTGACCCCATCCCGACCCGAACGTATATTACCTCATGAACATTCC
  ACGTGACCCCCGGTCCATCTACCTTGGNCGGAACGGGGAGAGCGAGGCTAACCTCCAGGC
  GCGCGATGGTGGGGACCCAGACTGTCCCCTCGGGCAGGTAGTTTGCCAGAGTTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004567 unedited
  AGCTATGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAT
  TTTTTAAAAAGTATTTATTGCAAAGAATGCTGGCACACAGTGTTGCATGTGGCTGAGATT
  CAATACCATCCATTCTGGGGAACTCGGGAAAATATCTGCAACATGGGCACGCCAGCCAGC
  ATGGCCGAAATGTGGCAGCCAATCAGCTACACCTCAGCCCATGCGGAGACCATGCCAGGG
  CAGAGGCCGGGGGGTTGCCCCCACTCATGCCCTGATCCCTGGATGTCCCTCTACCCTGTC
  CCCAGTCCTCGTAATGCATCCAGTCCAGCAACTCCTAACTCCCCTCTGTACACAGAGAGA
  AAAGGCCTCGGCGGTCTCACCTCAGATGACATCCAGAGGTACATGCACTGTTGCTGACCA
  AGGGAACCCACTATGGCTAACAAATGAGTGTGTCCAGGTGACAAAAGTCACCAAAACTCC
  AAAATACACAGAAGCAAAGGCTGCACTCCTAATGTCCCAAAGGCATGGTCCTTGGGCTCT
  GGGTCTAAAGGGCTTGGGGCTCTCACACAGAATCTGGAGGATCTTGCCCCCGGTCCCCCT
  GGACATTTCTTACCGAGCCCCCCCAGGCTGAGACACACCGACCCGGGCACGGGCCCTATC
  TTCAGTCGCGCTCCGGGGAGCTCTCGGGACTCGCCTAACCCGGGAACCTGGAAATATCGC
  CCTCCCTTTCGACATTGAACATCACGGGCGCGCGCGCCAGAGGCCACTCCCTCCATGGCA
  CCCGCCTACACGCCTCGTCGCCCCTTTCACCCATACCGACCCTCATCAACCGCCCCAACC
  CTACAATATTCACTCTACATCACCACACAACACCATTGTCACGTAACACCCGCACACCCC
  TCAGATTCCGCCGCCGGCCGGTCCCTGCTCCTTGGCCAGTACNAGCATCTAGCCGTACTC
  CACCCGCGTC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004567
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004567.2, NP_004558.1
• RefSeq Size: 3503 bp
• RefSeq ORF: 1410 bp
• Locus ID: 5210
• Cytogenetics: 3p21.31
• Domains: PGAM, 6PF2K
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism
• Gene Summary: 'The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (2) lacks an alternate coding exon in the 5' end compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.