CENPA (NM_001042426) Human Untagged Clone

CAT#: SC322212

CENPA (untagged)-Human centromere protein A (CENPA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CENPA
• Synonyms: CenH3; CENP-A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322212 AGCACCGGCTCATGTGAGGCTCGCGGCACAGCGTTCTCTGGGCTCCCCAGAAGCCAGCCT
  TTCGCTCCCGGACCCGGCAGCCCGAGCAGGAGCCGTGGGACCGGGCGCCAGCACCCTCTG
  CGGCGTGTCATGGGCCCGCGCCGCCGGAGCCGAAAGCCCGAGGCCCCGAGGAGGCGCAGC
  CCGAGCCCGACCCCGACCCCCGGCCCCTCCCGGCGGGGCCCCTCCTTAGGCGCTTCCTCC
  CATCAACACAGTCGGCGGAGACAAGGTTGGCTAAAGGAGATCCGAAAGCTTCAGAAGAGC
  ACACACCTCTTGATAAGGAAGCTGCCCTTCAGCCGCCTGGCAGCAGAAGCATTTCTAGTT
  CATCTCTTTGAGGACGCCTATCTCCTCACCTTACATGCAGGCCGAGTTACTCTCTTCCCA
  AAGGATGTGCAACTGGCCCGGAGGATCCGGGGCCTTGAGGAGGGACTCGGCTGAGCTCCT
  GCACCCAGTGTTTCTGTCAGTCTTTCCTGCTCAGCCAGGGGGGATGATACCGGGGACTCT
  CCAGAGCCATGACTAGATCCAATGGATTCTGCGATGCTGTCTGGACTTTGCTGTCTCTGA
  ACAGTATGTGTGTGTTGCTTTAAATATTTTTCTTTTTTTTGAGAAGGAGAAGACTGCATG
  ACTTTCCTCTGTAACAGAGGTAATATATGAGACAATCAACACCGTTCCAAAGGCCTGAAA
  ATAATTTTCAGATAAAGAGACTCCAAGGTTGACTTTAGTTTGTGAGTTACTCATGTGACT
  ATTTGAGGATTTTGAAAACATCAGATTTGCTGTGGTATGGGAGAAAAGGTTATGTACTTA
  TTATTTTAGCTCTTTCTGTAATATTTACATTTTTTACCATATGTACATTTGTACTTTTAT
  TTTACACATAAGGGAAAAAATAAGACCACTTTGAGCAGTTGCCTGGAAGGCTGGGCATTT
  CCATCATATAGACCTCTGCCCTTCAGAGTAGCCTCACCATTAGTGGCAGCATCATGTAAC
  TGAGTGGACTGTGCTTGTCAACGGATGTGTAGCTTTTCAGAAACTTAATTGGGGATGAAT
  AGAAAACCTGTAAGCTTTGATGTTCTGGTTACTTCTAGTAAATTCCTGTCAAAATCAATT
  CAGAAATTCTAACTTGGAGAATTTAACATTTTACTCTTGTAAATCATAGAAGATGTATCA
  TAACAGTTCAGAATTTTAAAGTACATTTTCGATGCTTTTATGGGTATTTTTGTAGTTTCT
  TTGTAGAGAGATAATAAAAATCAAAATATTTAATGAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001042426
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042426.1, NP_001035891.1
• RefSeq Size: 1352 bp
• RefSeq ORF: 345 bp
• Locus ID: 1058
• Cytogenetics: 2p23.3
• Gene Summary: 'Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting protein (isoform b) is shorter than isoform a.