RIP2 (RIPK2) (NM_003821) Human Untagged Clone

CAT#: SC323325

RIPK2 (untagged)-Kinase deficient mutant (K47M) of Human receptor-interacting serine-threonine kinase 2 (RIPK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RIPK2
• Synonyms: CARD3; CARDIAK; CCK; GIG30; RICK; RIP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323325 sequence for NM_003821 edited (data generated by NextGen Sequencing)
  ATGAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTACCACAAACTCGCCGAC
  CTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTGTGTCGTCCGCCCGCCACGCAGACTGG
  CGCGTCCAGGTGGCCGTGATGCACCTGCACATCCACACTCCGCTGCTCGACAGTGAAAGA
  AAGGATGTCTTAAGAGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCA
  ATTTTGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATGCCAAAT
  GGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGATGTTGCTTGGCCATTG
  AGATTTCGCATCCTGCATGAAATTGCCCTTGGTGTAAATTACCTGCACAATATGACTCCT
  CCTTTACTTCATCATGACTTGAAGACTCAGAATATCTTATTGGACAATGAATTTCATGTT
  AAGATTGCAGATTTTGGTTTATCAAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGT
  AGCAAATCTGCACCAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTATGAACCT
  GGACAAAAATCAAGGGCCAGTATCAAGCACGATATATATAGCTATGCAGTTATCACATGG
  GAAGTGTTATCCAGAAAACAGCCTTTTGAAGATGTCACCAATCCTTTGCAGATAATGTAT
  AGTGTGTCACAAGGACATCGACCTGTTATTAATGAAGAAAGTTTGCCATATGATATACCT
  CACCGAGCACGTATGATCTCTCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGA
  CCATCTTTCTTAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAAGAGATA
  ACTTTTCTTGAAGCTGTTATTCAGCTAAAGAAAACAAAGTTACAGAGTGTTTCAAGTGCC
  ATTTACCTATGTGACAAGAAGAAAATGGAATTATCTCTGAACATACCTGTAAATCATGGT
  CCACAAGAGGAATCATGTGGATCCTCTCAGCTCCATGAAAATAGTGGTTCTCCTGAAACT
  TCAAGGTCCCTGCCAGCTCCTCAAGACAATGATTTTTTATCTAGAAAAGCTCAAGACTGT
  TATTTTATGAAGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCACCATTTCTGGA
  TCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTCTTCAGCAATAATAAAT
  CCACTCTCAACTGCAGGAAACTCAGAACGTCTGCAGCCTGGTATAGCCCAGCAGTGGATC
  CAGAGCAAAAGGGAAGACATTGTGAACCAAATGACAGAAGCCTGCCTTAACCAGTCGCTA
  GATGCCCTTCTGTCCAGGGACTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAG
  CCTACAAGGACCTCAAAAGTCAGACAATTACTAGACACTACTGACATCCAAGGAGAAGAA
  TTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAACAAATGGGTCTTCAGCCTTAC
  CCGGAAATACTTGTGGTTTCTAGATCACCATCTTTAAATTTACTTCAAAATAAAAGCATG
  TAA
  
  Clone variation with respect to NM_003821.5
  140 a=>t;964 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003821 unedited
  AGGGTACGACGAACAACGAGCGGAGGCGAGAACGGGAGAGGTAACACAAGCAGAGCACGAAAGCGAACCG
  ACAGAACCACGAAACACGACCCACAAAAGGGCGGCCGCGAAAACGGCACGAGGGGCCACGCACGCGCAGG
  GGCGCAACAGGGCGCCCGAGCGCGGCGAGGGAGCCCTCGGGAGCCCGCCGCAGCAGGGGGCACACCCGGA
  ACCGGCCTGAGCGCCCCGGGACCATGAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTTCCCTACC
  ACAAACTCGCCGACCTGCGCTACCTGAGCCGCGGCGCCTCTGGGCACTGTGTCGTCCCGCCCCGCCACGC
  AGACTGGCGCGTCCAGGTGGGCCGGTGATGCCACCTGCACATCCCCACTCCCGCTGCTCGACAGTGGAAG
  GAAAGGATGTTCTAAGAAGAAGCTGAAATTTTACACAAAGCTAGGATTTAGTACATTCTTCAAATTTTGG
  GATTTTGCATGAAGCCTGATTTTTTGGAATAGTTACTGAATACATGGCAAATTGGTCCATTAAAGGAACT
  CTACCTTAGGAAACCTGGAATCTCTGAATGTGCCTGGCCAATTAAGTTTCGCAACCGCGTGTAAATTTCC
  CTTGTGTGTAATTCCTCGACAAATTGATCTCCCTTACTCTCATGACCTGGAATCTGAATCTCATTGGACA
  TAGATTCTGTGTAGATTGGCATTTGGTTTAAATGGCAGAGTGCCTCTCAGTCCAGTGCATTGCCCGAAGG
  GACAATTCTATGCACTGAACTTGACTGCAATACGCAATCAGCGATTAGCTGCTTCATGAAGTTATCGAAC
  GCCTGAGTACAACTCGCAAAGAT
  
  Kinase domain sequence

  >SC323325 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCCTTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCCTCGCTCGTGCAGGG
  GCGTATCTGGGCGCCTGAGCGCGGCGTGGGAGCCTTGGGAGCCGCCGCAGCAGGGGGCACACCCGGAACC
  GGCCTGAGCGCCCGGGACCATGAACGGGGAGGCCATCTGCAGCGC
  
• Restriction Sites: Please inquire
• ACCN: NM_003821
• ORF Size: 1623 bp
• Insert Size: 1880
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003821.4, NP_003812.1
• RefSeq Size: 2521
• RefSeq ORF: 1623
• Locus ID: 8767
• Domains: pkinase, TyrKc, CARD, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Neurotrophin signaling pathway, NOD-like receptor signaling pathway
• Gene Summary: This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]