RIP2 (RIPK2) (NM_003821) Human Untagged Clone

CAT#: SC323355

RIPK2 (untagged)-Kinase deficient mutant (K47M) of Human receptor-interacting serine-threonine kinase 2 (RIPK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RIPK2
• Synonyms: CARD3; CARDIAK; CCK; GIG30; RICK; RIP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003821, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTACCACAAACTCGCCGACCTGCGCTACC
  TGAGCCGCGGCGCCTCTGGCACTGTGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCGTGAA
  GCACCTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAGAGAAGCTGAAATTTTA
  CACAAAGCTAGATTTAGTTACATTCTTCCAATTTTGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAG
  TTACTGAATACATGCCAAATGGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGATGTTGC
  TTGGCCATTGAGATTTCGCATCCTGCATGAAATTGCCCTTGGTGTAAATTACCTGCACAATATGACTCCT
  CCTTTACTTCATCATGACTTGAAGACTCAGAATATCTTATTGGACAATGAATTTCATGTTAAGATTGCAG
  ATTTTGGTTTATCAAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGTAGCAAATCTGCACCAGAAGG
  AGGGACAATTATCTATATGCCACCTGAAAACTATGAACCTGGACAAAAATCAAGGGCCAGTATCAAGCAC
  GATATATATAGCTATGCAGTTATCACATGGGAAGTGTTATCCAGAAAACAGCCTTTTGAAGATGTCACCA
  ATCCTTTGCAGATAATGTATAGTGTGTCACAAGGACATCGACCTGTTATTAATGAAGAAAGTTTGCCATA
  TGATATACCTCACCGAGCACGTATGATCTCTCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGA
  CCATCTTTCTTAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAAGAGATAACTTTTCTTG
  AAGCTGTTATTCAGCTAAAGAAAACAAAGTTACAGAGTGTTTCAAGTGCCATTCACCTATGTGACAAGAA
  GAAAATGGAATTATCTCTGAACATACCTGTAAATCATGGTCCACAAGAGGAATCATGTGGATCCTCTCAG
  CTCCATGAAAATAGTGGTTCTCCTGAAACTTCAAGGTCCCTGCCAGCTCCTCAAGACAATGATTTTTTAT
  CTAGAAAAGCTCAAGACTGTTATTTTATGAAGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCAC
  CATTTCTGGATCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTCTTCAGCAATAATAAAT
  CCACTCTCAACTGCAGGAAACTCAGAACGTCTGCAGCCTGGTATAGCCCAGCAGTGGATCCAGAGCAAAA
  GGGAAGACATTGTGAACCAAATGACAGAAGCCTGCCTTAACCAGTCGCTAGATGCCCTTCTGTCCAGGGA
  CTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAGCCTACAAGGACCTCAAAAGTCAGACAATTA
  CTAGACACTACTGACATCCAAGGAGAAGAATTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAAC
  AAATGGGTCTTCAGCCTTACCCGGAAATACTTGTGGTTTCTAGATCACCATCTTTAAATTTACTTCAAAA
  TAAAAGCATGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003821 unedited
  CCCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGATCCGGGGAATGGG
  CGCCCTCGTGACCTAGTGTTGCGGGGCAAAAAGGGTCTTGCCGGCCTCGCTCGTGCAGGGGCGTATCTGG
  GCGCCTGAGCGCGGCGTGGGAGCCTTGGGAGCCGCCGCAGCAGGGGGCACACCCGGAACCGGCCTGAGCG
  CCCGGGGACCATGAACGGGGGAGGGCCATCTGCAGCGCCCTGCCCACCCATTTCCCTACCACAAACTCGG
  CCGAACCTGGCGCTACCTGAGCCCGGCGGCGGCCCTCTGGCACTGTGTCGCCCGCCCGGCCCCGCAAACT
  GGGCCCGTCCAATGTGGCCGTGAGGCACTGGCCCATCCACCCTCCCGTTGTTCGACGAAAAACTGAAAAT
  CTGGAGGTGGCTGGGCCATGGGAAATTCGCATCCTGCGGAATTTGCCCTTGGGTGTAATTTCCCTGCCCA
  ATGAAACCCCCCCCTACCTCCCCCGAGACTGGAAAACTCAAAATATCTTTTGGGCACAGAATTTCTGTTT
  TGAATATTCGATATTTGGTTTAACAGTGGGGCGCGTGGTGCCCCTTCCACAGCCAAGTATACATCTGTCC
  CCCAAGGGGGGGACTTTATATGCGCCCTGTGAACAATAAACTGGGGAAAATATAGGGCGCATCTCACCAG
  AATATACTCTCCGCTATCACAGGGAGTGTGGTCTCCAGAAGCGTTGAGAGTATCCATCCTTCTGCAATGT
  AGATGTCCACGACGCACCCGTCTTATGAAAGTTGCAGTGATTATACTACCGACGCTGATGTACTCTACTC
  TGATGGACAGTCTCATAATACACATCTTCT
  
  Kinase domain sequence

  >SC323355 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYTTGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGATCCGGGGAATGGGCGCCCTC
  GTGACCTAGTGTTGCGGGGCAAAAAGGGTCTTGCCGGCCTCGCTCGTGCAGGGGCGTATCTGGGCGCCTG
  AGCGCGGCGTGGGAGCCTTGGGAGCCGCCGCAGCAGGGGGCACAC
  
• Restriction Sites: Please inquire
• ACCN: NM_003821
• ORF Size: 1623 bp
• Insert Size: 1880
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003821.4, NP_003812.1
• RefSeq Size: 2521
• RefSeq ORF: 1623
• Locus ID: 8767
• Domains: pkinase, TyrKc, CARD, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Neurotrophin signaling pathway, NOD-like receptor signaling pathway
• Gene Summary: This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]