NS1 (NC_012729) Virus Tagged ORF Clone

CAT#: VC100552

Myc-DDK-tagged ORF clone of viral ORF for non structural protein NS1 [Human bocavirus 4], codon optimized for human cell expression, YP_002916060

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100552 represents NCBI reference of YP_002916060 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCTCTGCGCCCGTTCTGAGGGCCTTCTCTCAGCCCACCTTTACCTACGTCATTAAATTCCCTT
  ACAATAACTGGAAAGAGGACGAGCACCTTCTGTGGTCCCTGCTTGCTCCGGGCACAGAATCCCTGATGAT
  CCAGCTCAAAAACTGCGCCCCTCATCCTGAAGATGACCCAATCAGAGAAGATATCCTCTGCAGCCTGGCT
  GACCTCCATTACGGGGCTGTTTTCGCTAAAGCCTGTTATATAGCCACTTCCACATTGATGGGCCAAAAGC
  AGAGGACTCTGTTCCCTCGCTGTGATATTGTGTGTCAATCTGAAATTGGAAGTGATTTTCTCCATTGTCA
  CATACTCGTGGGAGGAGCCGGCCTCAGCAAACGCAATGCCAAAATTTCCAGAGCAACTCTTTTGGGTTTG
  GTAATGGCAGAACTTACCCAGAGATGCAAACTCCTTCTTGCTCACAGGCCCTTTGAGCCGGCGGAGGCCA
  CCATCTACCATGAACTGAAAAGAATCGAAAGAGAAGCTTGGTCTGGACACACCGGGAACTGGGTGCAGAT
  TCTGCAATATAAAGACAAGAGAGGCGACCTCCATGCCCAACCTATTGACCCACTGCGGTTCCTGAAACAT
  TATATCCTGCCGAAGAACCGACTCATCAGCCCAAGCAGCAAGCCTGATGTCTGCACTTCCCCCGATAATT
  GGTTCATCCTCGCGGATAAGACATACTCCCACACAATTATTAATGGTCTCCCCCTGCTGGAAAGGAACCG
  GAAGGCCTACCTGCAGGAGTTGGAAAGCGAGGTCATTCCCGGACCGTCCGCTATGGCATTCGGCGGCAGA
  GGGGCCTGGGAGCAGCTCCCCGAGGTGGGGGAACAGAGGCTGATCACAAGTAACACCTCCACCGCTTACA
  AGGCGAATAAAAAGGAAAAGTTGATGCTGAACCTGCTGGACAAGTGCGATGAGCTTAATCTCCTCGTGTA
  TGAGGACCTGGTGTCCGCGTGCCCAGACCTGCTCTTGATGCTCGAGGGCCAACCTGGTGGAGCAAGGCTG
  ATTGAGCAGGTCCTGGGAATGCACCATATTAAAGTGTGCGCCAAACACACAGCCCTGAGTTTCTTGTTCC
  ACCTTCACCCAGACCAACTGCTGACGTCAAGCAATAAAGCACTCAAATTGCTGCTCATTCAGGGATATAA
  CCCTCTGCAAGTCGGACATGCTATCTGCTGCGTCCTCAATAAGCAAATGGGAAAGCAGAACACCATCTGT
  TTTTATGGCCCTGCATCTACCGGCAAAACCAACTTCGCCAAGGCCATAGTCCAGGGGGTCAGACTCTATG
  GGTGTGTAAACCACTTGAATAAGGGGTTCGTGTTTAACGATTGCCGCCAAAGACTGATCATCTGGTGGGA
  AGAATGCCTTATGCACCAGGACTGGGTCGAGCCCGCGAAATGTATTCTGGGCGGCACCGAGTGCCGAATT
  GATGTCAAGCACAAGGACAGTGTCCTTTTGCAGCAGACCCCTGTTATCATCAGCACCAACCACGATATAT
  ACTCCGTCGTAGGAGGAAACACAGTGTCTCACGTCCACGCGGCCCCTCTGAAGGAACGAGTGCTCCAGCT
  TAACTTTATGAAGCAGCTGCCTCAGACCTTCGGCGAAATCTCACCAAGCGAGATCGCAGAACTTCTGCAA
  TGGTGTTTTAACGAGTACGACTGTACCCTGGCTGGCTTTAAACAGAAATGGAACTTGGACAAGGTGCCAA
  ATAGCTTTCCGATCGGAGATTTGTGCCCAACCCACAGTCAGGACTTTACACTTCACGAAAATGGCTTCTG
  TAGCGACTGCGGCGGTTACCTGCCCCATAGCGCCGATGATAGCGTGTACACAGACGTGGCCAGCGAAACT
  ACGTCAGGAGATTACGATCCAGGCCGGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100552 representing YP_002916060
  Red=Cloning sites Green=Tags
  
  MAFSAPVLRAFSQPTFTYVIKFPYNNWKEDEHLLWSLLAPGTESLMIQLKNCAPHPEDDPIREDILCSLA
  DLHYGAVFAKACYIATSTLMGQKQRTLFPRCDIVCQSEIGSDFLHCHILVGGAGLSKRNAKISRATLLGL
  VMAELTQRCKLLLAHRPFEPAEATIYHELKRIEREAWSGHTGNWVQILQYKDKRGDLHAQPIDPLRFLKH
  YILPKNRLISPSSKPDVCTSPDNWFILADKTYSHTIINGLPLLERNRKAYLQELESEVIPGPSAMAFGGR
  GAWEQLPEVGEQRLITSNTSTAYKANKKEKLMLNLLDKCDELNLLVYEDLVSACPDLLLMLEGQPGGARL
  IEQVLGMHHIKVCAKHTALSFLFHLHPDQLLTSSNKALKLLLIQGYNPLQVGHAICCVLNKQMGKQNTIC
  FYGPASTGKTNFAKAIVQGVRLYGCVNHLNKGFVFNDCRQRLIIWWEECLMHQDWVEPAKCILGGTECRI
  DVKHKDSVLLQQTPVIISTNHDIYSVVGGNTVSHVHAAPLKERVLQLNFMKQLPQTFGEISPSEIAELLQ
  WCFNEYDCTLAGFKQKWNLDKVPNSFPIGDLCPTHSQDFTLHENGFCSDCGGYLPHSADDSVYTDVASET
  TSGDYDPGRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012729
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012729.2, YP_002916060
• RefSeq ORF: 1920
• Locus ID: 7922600
• MW: 71.8 kDa