NP1 (NC_012729) Virus Tagged ORF Clone

CAT#: VC100554

Myc-DDK-tagged ORF clone of viral ORF for NP1 [Human bocavirus 4], codon optimized for human cell expression, YP_002916061

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100554 represents NCBI reference of YP_002916061 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCTGAAAGCACAAAGAACAGACACCGGTCAAGCAAGCGAACACCCTCACCTCTGCAGAAGGAGC
  GCAAACGCAATTGGGAAAATAGAAAGAGTCGCTCCCGATCTCCTATTAGACGCCACGGTGAGAAAAATCT
  TGAATACGCTCATCACAATAACCAAGACAATAGACAGTCTAGCTATACAGCGTCCAAGACCTCAGACCAA
  GCGATGAAAACCAAAGAAAAGACAAGTGGCGGGACAAGGACCAACCCTTATACTGTTTTTAGTCAGCACA
  GGGCAAATCACAGCAACGCACCTGGCTGGTGTGGGTTTTACTGGCATTCCACTCGGCTGGCCCGCAACGG
  GACGAATAATATTTTCAACGAAATGAAGCAGAAGTTCCAGGAGCTGCAGATTGATGGCAAGATAAGCTGG
  GATACTACTCGCGAGTTGCTCTTTACGCAAAAAAAGACTTTGGATCAGGGCTATAGAAACATGCTGTATC
  ATTTCAGACATAGTCCTGATTGCCCGAGGTGTGATTACTGGGATGATGTGTATCGGAAGCACCTCGCCAA
  CGTGAGTTCCCAGGAGTCCGAGGAAGTCACCGATGAGGAAATGTTGAGCGCCGTCGAATCAATGGAAACA
  AATGCGAGCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100554 representing YP_002916061
  Red=Cloning sites Green=Tags
  
  MSSESTKNRHRSSKRTPSPLQKERKRNWENRKSRSRSPIRRHGEKNLEYAHHNNQDNRQSSYTASKTSDQ
  AMKTKEKTSGGTRTNPYTVFSQHRANHSNAPGWCGFYWHSTRLARNGTNNIFNEMKQKFQELQIDGKISW
  DTTRELLFTQKKTLDQGYRNMLYHFRHSPDCPRCDYWDDVYRKHLANVSSQESEEVTDEEMLSAVESMET
  NASN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012729
• ORF Size: 642 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012729.2, YP_002916061
• RefSeq ORF: 642
• Locus ID: 7922601
• MW: 25.2 kDa