NS2 (NC_012729) Virus Tagged ORF Clone

CAT#: VC100553

Myc-DDK-tagged ORF clone of viral ORF for non structural protein NS2 [Human bocavirus 4], codon optimized for human cell expression, YP_002916059

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100553 represents NCBI reference of YP_002916059 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTTAGCGCACCGGTCCTTAGGGCCTTCAGCCAGCCAACCTTCACATATGTGATCAAGTTCCCTT
  ATAATAATTGGAAAGAAGACGAGCACCTGCTGTGGTCCCTGCTGGCCCCAGGAACTGAATCACTGATGAT
  TCAGCTGAAAAACTGCGCGCCGCATCCCGAGGACGACCCTATTAGAGAGGATATCCTGTGTAGTCTGGCC
  GACCTGCATTACGGAGCCGTGTTTGCGAAGGCTTGTTATATCGCCACCAGCACACTTATGGGCCAGAAGC
  AGCGCACCCTGTTTCCCCGGTGTGACATTGTCTGCCAAAGCGAGATAGGGTCCGACTTTCTGCATTGCCA
  CATTCTGGTGGGCGGTGCAGGTCTGTCCAAACGGAATGCCAAAATTAGCCGGGCTACTCTCTTGGGACTG
  GTGATGGCTGAACTTACGCAGCGCTGTAAGCTGTTGCTCGCCCACAGACCGTTCGAGCCAGCAGAGGCTA
  CCATTTATCATGAGCTTAAAAGGATCGAAAGAGAAGCCTGGTCAGGCCATACCGGCAATTGGGTCCAGAT
  CTTGCAATACAAAGACAAGCGGGGGGACCTGCACGCCCAACCCATCGACCCACTGCGCTTTCTGAAACAC
  TATATCCTCCCAAAGAATAGACTGATATCACCGAGCAGCAAGCCTGATGTGTGTACGAGTCCCGATAACT
  GGTTCATCCTGGCCGATAAAACATACTCTCATACCATAATCAACGGCCTCCCACTGCTGGAGCGGAACAG
  AAAGGCTTACCTGCAGGAGCTGGAATCCGAGGTGATACCTGGACCTTCAGCAATGGCCTTCGGAGGTAGA
  GGGGCATGGGAGCAACTCCCTGAGGTTGGGGAACAGCGACTGATCACTAGCAACACATCCACTGCTTACA
  AAGCAAATAAGAAAGAGAAATTGATGCTCAATCTTCTGGATAAATGCGACGAGCTGAATCTCCTTGTGTA
  CGAGGATCTTGTCTCCGCGTGCCCGGATCTGTTGCTGATGCTCGAGGGTCAGCCAGGAGGGGCTCGATTG
  ATAGAACAGGTGCTGGGAATGCACCATATTAAAGTCTGTGCAAAGCATACTGCCCTGTCCTTTTTGTTTC
  ACCTCCACCCAGACCAGCTCCTCACCTCCTCCAATAAGGCACTCAAGTTGTTGCTTATCCAAGGATATAA
  TCCGCTGCAAGTGGGCCACGCCATATGTTGCGTCCTGAATAAACAGATGGGGAAGCAGAATACCATTTGT
  TTTTACGGACCTGCTAGTACAGGGAAAACCAATTTCGCTAAAGCTATTGTACAGGGCGTGAGGCTCTATG
  GATGCGTTAACCACCTTAATAAAGGGTTTGTGTTCAACGATTGCAGACAACGGCTGATCATCTGGTGGGA
  GGAATGCCTGATGCATCAGGACTGGGTGGAGCCCGCGAAATGCATCCTCGGGGGCACAGAATGCCGGATT
  GACGTGAAACATAAGGATTCCGTCCTCCTGCAGCAAACCCCCGTGATAATTTCCACGAATCATGACATTT
  ATAGCGTCGTGGGAGGGAACACGGTCTCCCACGTGCACGCCGCCCCCTTGAAGGAAAGGGTGCTGCAACT
  GAATTTCATGAAGCAACTTCCACAGACTTTTGGAGAAATTAGCCCTAGTGAAATCGCAGAATTGCTCCAG
  TGGTGTTTCAACGAGTATGATTGCACACTTGCTGGATTCAAGCAGAAGTGGAATCTCGACAAAGTGCCTA
  ATTCTTTTCCCATAGGGGACTTGTGCCCTACTCATAGTCAAGATTTTACCCTCCACGAAAACGGCTTTTG
  TAGTGACTGTGGCGGATATCTCCCTCATAGTGCTGACGACAGTGTGTATACTGACGTGGCAAGCGAGACT
  ACTTCAGGAGATTACGACCCGGGTAACTTGGGGGACACTGATGGCGAGGACTCTAAGTCTGAGGCATCCG
  AGGTGGACTATTGCCCGCCGAAGAAGCGGCGAGTTATCAGCGCGACTCCCCCCAATTCACCAGTGTCAGG
  TCCCAGTTTGTCTACCTTCTTGGATACCTGGCAGTCCCAGCCACGGGACGATGACGAGCTGCGCATCTAC
  GAGGAGCAGGCATCCCAGTTCCAGAAGAATACTAAGAGTACAAGCGAGCGCGAAGAGGCCCAACTCGGGG
  AGTCCCAGGAACCGCAACCTGAGCCAGACCCGACAGCTTGGGGAGAGAAGCTGGGCGTTTGTTCCAGTCA
  GCAACCCGGCCAGCCTCCAATCGTGCTGTATTGCTTTGAGGACCTCCGGCCAAGTGACGAAGATGAAGGT
  GAAAACATCGGCGGAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100553 representing YP_002916059
  Red=Cloning sites Green=Tags
  
  MAFSAPVLRAFSQPTFTYVIKFPYNNWKEDEHLLWSLLAPGTESLMIQLKNCAPHPEDDPIREDILCSLA
  DLHYGAVFAKACYIATSTLMGQKQRTLFPRCDIVCQSEIGSDFLHCHILVGGAGLSKRNAKISRATLLGL
  VMAELTQRCKLLLAHRPFEPAEATIYHELKRIEREAWSGHTGNWVQILQYKDKRGDLHAQPIDPLRFLKH
  YILPKNRLISPSSKPDVCTSPDNWFILADKTYSHTIINGLPLLERNRKAYLQELESEVIPGPSAMAFGGR
  GAWEQLPEVGEQRLITSNTSTAYKANKKEKLMLNLLDKCDELNLLVYEDLVSACPDLLLMLEGQPGGARL
  IEQVLGMHHIKVCAKHTALSFLFHLHPDQLLTSSNKALKLLLIQGYNPLQVGHAICCVLNKQMGKQNTIC
  FYGPASTGKTNFAKAIVQGVRLYGCVNHLNKGFVFNDCRQRLIIWWEECLMHQDWVEPAKCILGGTECRI
  DVKHKDSVLLQQTPVIISTNHDIYSVVGGNTVSHVHAAPLKERVLQLNFMKQLPQTFGEISPSEIAELLQ
  WCFNEYDCTLAGFKQKWNLDKVPNSFPIGDLCPTHSQDFTLHENGFCSDCGGYLPHSADDSVYTDVASET
  TSGDYDPGNLGDTDGEDSKSEASEVDYCPPKKRRVISATPPNSPVSGPSLSTFLDTWQSQPRDDDELRIY
  EEQASQFQKNTKSTSEREEAQLGESQEPQPEPDPTAWGEKLGVCSSQQPGQPPIVLYCFEDLRPSDEDEG
  ENIGGD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012729
• ORF Size: 2328 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012729.2, YP_002916059
• RefSeq ORF: 2328
• Locus ID: 7922604
• MW: 86.8 kDa