VP1 (NC_012729) Virus Tagged ORF Clone

CAT#: VC100555

Myc-DDK-tagged ORF clone of viral ORF for capsid protein VP1 [Human bocavirus 4], codon optimized for human cell expression, YP_002916062

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100555 represents NCBI reference of YP_002916062 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCCATCAAAAGACAGCCTGGAGGCTGGGTGTTGCCTGGGTATAAGTATCTCGGCCCGTTCAATC
  CTCTCGAGAACGGAGAACCGGTGAACAAGGCGGACAGGGCAGCTCAGGCCCACGATAAGTCTTATTCTGA
  GCTGATTAAGAGCGGCAAGAACCCTTACCTCTATTTCAATAAGGCCGACGAGAAGTTCATCGATGACCTC
  AAAGATGACTGGTCACTGGGGGGGATAATCGGTAGCTCATTCTTCAAGCTTAAAAGAGCCGTTGCTCCGG
  CACTCGGAAACAAAGAACGGGCTCAGAAGAGACATTTCTATTTTGCTAATAGCAATAAAGGGGCTAAAAA
  GACTAAGAACAATGAGCCCAAGCCTGGGACGAGTAAGATGTCTGAAAATGAAATTCAGGACCAGCAGCCC
  TCAGACAGCATGGATGGCCAGAGAGGTGGGGGTGGCGGTGCCACCGGCTCTGTTGGCGGCGGCAAAGGAT
  CTGGCGTTGGAATTTCTACCGGTGGCTGGGTAGGAGGCTCCTATTTTACAGATAGCTATGTAATTACTAA
  AAATACAAGACAGTTTCTGGTGAAGATACAGAACAATCACCAATACAAAACTGAACTGATCAGCCCTTCT
  ACCTCCCAAGGGAAAAGCCAACGCTGCGTATCCACCCCCTGGAGCTATTTCAACTTTAATCAGTATTCCT
  CCCATTTTTCTCCACAAGACTGGCAACGGTTGACCAACGAATACAAGAGATTCCGCCCTAAAGGGATGCA
  TGTAAAGATATACAATCTTCAGATCAAGCAAATACTGTCAAACGGGGCCGACACAACGTATAATAACGAT
  TTGACTGCTGGGGTCCACATCTTCTGTGACGGAGAGCACGCCTACCCTAACGCCACTCATCCCTGGGATG
  AAGATGTGATGCCGGAGCTGCCTTATCAGACTTGGTACCTGTTTCAGTATGGCTACATACCCGTGATTCA
  CGAATTGGCAGAGATGGAAGATTCCAACGCCGTTGAAAAAGCCATCTGCCTGCAGATTCCGTTCTTTATG
  CTCGAGAATAGTGACCATGAGGTGTTGCGCACGGGAGAAAGTACAGAATTCACGTTTAACTTTGATTGTG
  AGTGGATTAATAATGAACGGGCATACATTCCTCCTGGCCTTATGTTCAATCCACTCGTGCCAACCCGGCG
  AGCTCAATACATCCGGCGAAATAATAACCCTCAGACAGCTGAGAGCACTTCCCGAATTGCACCATATGCC
  AAACCGACATCCTGGATGACCGGACCTGGCCTGCTGAGCGCGCAGAGAGTCGGACCTGCCACATCTGATA
  CGGGCGCTTGGATGGTGGCCGTCAAGCCCGAGAACGCCAGTATTGATACAGGCATGTCCGGCATTGGCAG
  TGGCTTCGATCCCCCCCAAGGTTCCCTGGCCCCTACCAATCTGGAATACAAAATTCAGTGGTATCAAACA
  CCTCAGGGCACAAATAATAATGGGAACATCATTTCAAATCAACCTCTTTCAATGCTTCGCGATCAGGCAT
  TGTTCAGGGGGAATCAGACTACCTATAATTTGTGCTCTGACGTTTGGATGTTCCCCAACCAAATCTGGGA
  TCGGTACCCCATTACCAGGGAAAATCCAATTTGGTGTAAAAAGCCAAGGTCAGATAAACACACCACCATA
  GACCCATTCGATGGCAGTCTGGCAATGGACCATCCTCCGGGCACAATATTCATTAAGATGGCAAAAATAC
  CAGTGCCTTCCAACAATAACGCAGATTCTTATCTGAACATTTACTGCACCGGACAAGTGTCATGTGAGAT
  AGTGTGGGAGGTAGAGAGATATGCTACGAAAAACTGGAGACCCGAACGGAGGCACACTACCTTCGGCCTT
  GGCATTGGAGGCGCCGACAACCTGAATCCCACTTACCACGTGGACAAGAATGGCACCTACATACAACCTA
  CAACCTGGGACATGTGCTTTCCTGTGAAGACTAACATCAACAAAGTCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100555 representing YP_002916062
  Red=Cloning sites Green=Tags
  
  MPPIKRQPGGWVLPGYKYLGPFNPLENGEPVNKADRAAQAHDKSYSELIKSGKNPYLYFNKADEKFIDDL
  KDDWSLGGIIGSSFFKLKRAVAPALGNKERAQKRHFYFANSNKGAKKTKNNEPKPGTSKMSENEIQDQQP
  SDSMDGQRGGGGGATGSVGGGKGSGVGISTGGWVGGSYFTDSYVITKNTRQFLVKIQNNHQYKTELISPS
  TSQGKSQRCVSTPWSYFNFNQYSSHFSPQDWQRLTNEYKRFRPKGMHVKIYNLQIKQILSNGADTTYNND
  LTAGVHIFCDGEHAYPNATHPWDEDVMPELPYQTWYLFQYGYIPVIHELAEMEDSNAVEKAICLQIPFFM
  LENSDHEVLRTGESTEFTFNFDCEWINNERAYIPPGLMFNPLVPTRRAQYIRRNNNPQTAESTSRIAPYA
  KPTSWMTGPGLLSAQRVGPATSDTGAWMVAVKPENASIDTGMSGIGSGFDPPQGSLAPTNLEYKIQWYQT
  PQGTNNNGNIISNQPLSMLRDQALFRGNQTTYNLCSDVWMFPNQIWDRYPITRENPIWCKKPRSDKHTTI
  DPFDGSLAMDHPPGTIFIKMAKIPVPSNNNADSYLNIYCTGQVSCEIVWEVERYATKNWRPERRHTTFGL
  GIGGADNLNPTYHVDKNGTYIQPTTWDMCFPVKTNINKVL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012729
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012729.2, YP_002916062
• RefSeq ORF: 2010
• Locus ID: 7922602
• MW: 75.4 kDa