NS1 (NC_007455) Virus Tagged ORF Clone

CAT#: VC100557

Myc-DDK-tagged ORF clone of viral ORF for NS1 [Human bocavirus], codon optimized for human cell expression, YP_338086

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100557 represents NCBI reference of YP_338086 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTCAATCCGCCTGTGATAAGAGCCTTCAGCCAGCCTGCCTTTACTTATGTGTTTAAGTTCCCCT
  ATCCACAGTGGAAAGAGAAAGAGTGGCTGCTTCACGCCCTCCTGGCCCACGGAACCGAGCAGTCCATGAT
  TCAGCTGCGAAACTGCGCCCCTCATCCAGACGAAGACATTATTCGAGATGACCTTTTGATCTCACTGGAA
  GACCGGCATTTTGGTGCAGTACTGTGTAAAGCCGTTTATATGGCCACAACTACACTTATGTCCCACAAGC
  AGCGAAACATGTTCCCCAGATGTGATATCATTGTGCAGTCCGAACTCGGCGAGAAAAATCTGCACTGTCA
  CATTATAGTGGGAGGCGAGGGCCTGAGCAAGCGCAACGCAAAGTCAAGTTGTGCACAATTCTATGGACTG
  ATTTTGGCTGAGATAATTCAGCGCTGTAAATCCCTTTTGGCAACCCGCCCCTTCGAACCCGAGGAAGCTG
  ACATTTTTCATACTCTGAAAAAGGCTGAAAGAGAGGCATGGGGCGGAGTGACAGGCGGAAACATGCAGAT
  CTTGCAGTATAGGGACAGACGAGGCGACCTCCACGCTCAAACAGTAGATCCCCTGCGGTTCTTCAAGAAT
  TATCTGCTTCCCAAAAATAGGTGTATCTCCTCATACTCCAAGCCCGATGTGTGCACGTCACCTGACAACT
  GGTTCATTCTGGCCGAAAAGACTTACAGCCATACTTTGATAAACGGACTGCCTCTGCCGGAGCACTATCG
  GAAGAATTATCACGCCACTCTGGACAACGAGGTAATACCGGGACCTCAGACGATGGCCTACGGAGGCAGG
  GGACCCTGGGAGCACCTGCCCGAAGTGGGCGATCAGCGGCTCGCAGCAAGCAGCGTGTCCACAACTTATA
  AACCAAATAAGAAAGAAAAGCTTATGCTGAACCTTCTGGACAAGTGCAAGGAACTGAACTTGTTGGTTTA
  TGAAGACCTCGTGGCAAATTGCCCTGAGCTTCTCCTGATGCTGGAAGGCCAGCCTGGCGGGGCTAGACTT
  ATTGAACAGGTGTTGGGCATGCACCACATTAATGTGTGCTCTAACTTCACCGCGCTTACCTACCTCTTCC
  ACCTGCACCCTGTGACTTCCCTGGACTCCGACAATAAGGCACTGCAGCTGCTGCTGATCCAGGGGTACAA
  TCCTCTTGCAGTAGGCCACGCTCTGTGTTGCGTACTCAACAAACAGTTTGGGAAGCAAAACACTGTGTGC
  TTTTATGGTCCGGCATCCACTGGGAAGACCAACATGGCAAAAGCTATTGTCCAGGGGATCCGGCTCTATG
  GTTGTGTGAACCATCTGAACAAAGGGTTTGTGTTTAATGACTGTCGCCAGAGGCTGGTCGTGTGGTGGGA
  AGAGTGTCTGATGCATCAAGACTGGGTGGAACCAGCCAAGTGCATCCTTGGCGGAACCGAATGCAGGATT
  GACGTGAAGCACAGGGACAGCGTCCTGCTGACACAAACCCCGGTAATCATCAGCACGAATCACGACATTT
  ATGCCGTGGTCGGAGGCAACTCCGTGTCACACGTACATGCGGCGCCCCTTAAAGAACGGGTGATTCAACT
  TAACTTTATGAAGCAGCTCCCGCAGACATTCGGGGAGATCACTGCCACTGAGATTGCCGCGCTTCTTCAG
  TGGTGCTTTAACGAGTACGATTGTACTCTCACAGGCTTCAAGCAAAAGTGGAATCTTGACAAGATTCCAA
  ATTCATTTCCACTGGGAGTGCTCTGTCCGACCCATTCCCAGGATTTTACCTTGCATGAGAACGGCTATTG
  TACTGACTGCGGGGGCTACCTCCCCCACAGCGCTGATAATAGCATGTACACAGATCGCGCGTCCGAAACA
  AGCACAGGAGACATCACTCCCTCAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100557 representing YP_338086
  Red=Cloning sites Green=Tags
  
  MAFNPPVIRAFSQPAFTYVFKFPYPQWKEKEWLLHALLAHGTEQSMIQLRNCAPHPDEDIIRDDLLISLE
  DRHFGAVLCKAVYMATTTLMSHKQRNMFPRCDIIVQSELGEKNLHCHIIVGGEGLSKRNAKSSCAQFYGL
  ILAEIIQRCKSLLATRPFEPEEADIFHTLKKAEREAWGGVTGGNMQILQYRDRRGDLHAQTVDPLRFFKN
  YLLPKNRCISSYSKPDVCTSPDNWFILAEKTYSHTLINGLPLPEHYRKNYHATLDNEVIPGPQTMAYGGR
  GPWEHLPEVGDQRLAASSVSTTYKPNKKEKLMLNLLDKCKELNLLVYEDLVANCPELLLMLEGQPGGARL
  IEQVLGMHHINVCSNFTALTYLFHLHPVTSLDSDNKALQLLLIQGYNPLAVGHALCCVLNKQFGKQNTVC
  FYGPASTGKTNMAKAIVQGIRLYGCVNHLNKGFVFNDCRQRLVVWWEECLMHQDWVEPAKCILGGTECRI
  DVKHRDSVLLTQTPVIISTNHDIYAVVGGNSVSHVHAAPLKERVIQLNFMKQLPQTFGEITATEIAALLQ
  WCFNEYDCTLTGFKQKWNLDKIPNSFPLGVLCPTHSQDFTLHENGYCTDCGGYLPHSADNSMYTDRASET
  STGDITPSK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007455
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007455.1, YP_338086
• RefSeq ORF: 1917
• Locus ID: 3711585
• MW: 72.1 kDa