VP1 (NC_007455) Virus Tagged ORF Clone

CAT#: VC100559

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human bocavirus], codon optimized for human cell expression, YP_338088

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100559 represents NCBI reference of YP_338088 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCTATCAAGAGACAGCCCCGAGGGTGGGTCCTGCCCGGTTACAGGTATCTGGGTCCATTCAACC
  CTTTGGACAACGGAGAGCCTGTCAATAATGCAGATCGCGCCGCACAGCTGCACGACCATGCCTATAGCGA
  GCTGATCAAATCCGGCAAGAATCCATACCTGTACTTTAACAAAGCAGATGAGAAGTTCATCGACGATCTG
  AAGGATGACTGGTCCATAGGCGGCATTATTGGCAGCAGCTTCTTTAAGATCAAACGAGCAGTTGCACCCG
  CTCTCGGTAACAAGGAGAGGGCACAGAAGAGGCACTTTTACTTTGCTAACTCCAATAAAGGAGCAAAAAA
  GACCAAAAAATCTGAACCCAAGCCGGGCACCAGCAAGATGAGTGACACCGATATACAGGACCAGCAGCCG
  GACACTGTAGACGCTCCTCAAAATACTAGTGGGGGGGGCACTGGGTCTATAGGTGGAGGTAAGGGGTCTG
  GCGTGGGAATTTCCACCGGAGGGTGGGTTGGCGGGAGTCATTTCAGCGACAAGTACGTGGTGACTAAAAA
  CACCCGGCAGTTTATTACCACAATTCAAAATGGCCATCTGTACAAGACGGAGGCAATCGAAACAACAAAT
  CAGAGCGGAAAGAGCCAGCGGTGCGTGACTACCCCCTGGACCTATTTTAACTTCAACCAGTATTCCTGCC
  ACTTTAGCCCTCAAGATTGGCAGCGGCTCACCAACGAATACAAACGCTTTCGGCCAAAGGCAATGCAGGT
  AAAGATCTATAACCTGCAGATTAAGCAAATCCTCTCCAATGGCGCCGATACAACATACAACAATGATCTG
  ACAGCGGGGGTTCACATTTTCTGTGACGGCGAACACGCTTATCCCAACGCTTCACACCCGTGGGACGAGG
  ATGTGATGCCCGACCTGCCGTACAAGACTTGGAAACTTTTTCAGTATGGATACATTCCCATTGAGAATGA
  ACTCGCTGACCTCGATGGCAATGCTGCAGGTGGGAATGCGACCGAGAAGGCACTTCTCTACCAGATGCCT
  TTTTTTCTCCTGGAGAATTCAGATCACCAGGTCCTTCGCACTGGTGAGAGTACAGAGTTCACTTTCAATT
  TCGATTGCGAATGGGTCAACAATGAGAGAGCTTACATCCCTCCGGGGCTTATGTTCAATCCTAAAGTCCC
  TACCCGGAGGGTGCAGTATATCCGGCAGAACGGGTCCACGGCAGCCTCTACTGGCCGCATCCAACCCTAT
  TCCAAGCCCACATCCTGGATGACCGGACCTGGGCTGCTGTCAGCTCAAAGAGTCGGACCACAGAGCTCAG
  ACACCGCACCTTTCATGGTTTGTACCAATCCGGAAGGAACTCACATTAACACTGGTGCAGCAGGATTCGG
  CAGTGGTTTCGATCCCCCCAATGGCTGCTTGGCCCCAACAAATCTTGAATACAAACTGCAATGGTACCAG
  ACCCCGGAAGGTACCGGCAACAATGGAAATATCATTGCCAATCCCTCCCTGAGTATGCTTCGAGATCAAT
  TGTTGTACAAGGGCAACCAGACCACTTATAACCTTGTCGGTGACATCTGGATGTTTCCCAACCAGGTATG
  GGACAGGTTTCCTATCACCAGGGAAAATCCCATATGGTGCAAAAAACCGCGCGCAGATAAACATACAATT
  ATGGATCCTTTCGATGGGAGTATTGCCATGGACCATCCACCTGGCACAATCTTCATCAAGATGGCCAAAA
  TCCCAGTGCCAACCGCTTCAAATGCTGACTCCTACTTGAATATCTACTGTACAGGGCAGGTGTCTTGTGA
  AATTGTTTGGGAAGTGGAAAGATACGCCACCAAAAATTGGAGGCCTGAAAGGCGACATACAGCCCTCGGC
  ATGTCACTGGGTGGAGAGAGTAATTACACCCCAACATACCATGTGGACCCGACGGGAGCCTACATCCAGC
  CCACCTCTTATGACCAGTGTATGCCCGTGAAAACAAACATCAACAAGGTACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100559 representing YP_338088
  Red=Cloning sites Green=Tags
  
  MPPIKRQPRGWVLPGYRYLGPFNPLDNGEPVNNADRAAQLHDHAYSELIKSGKNPYLYFNKADEKFIDDL
  KDDWSIGGIIGSSFFKIKRAVAPALGNKERAQKRHFYFANSNKGAKKTKKSEPKPGTSKMSDTDIQDQQP
  DTVDAPQNTSGGGTGSIGGGKGSGVGISTGGWVGGSHFSDKYVVTKNTRQFITTIQNGHLYKTEAIETTN
  QSGKSQRCVTTPWTYFNFNQYSCHFSPQDWQRLTNEYKRFRPKAMQVKIYNLQIKQILSNGADTTYNNDL
  TAGVHIFCDGEHAYPNASHPWDEDVMPDLPYKTWKLFQYGYIPIENELADLDGNAAGGNATEKALLYQMP
  FFLLENSDHQVLRTGESTEFTFNFDCEWVNNERAYIPPGLMFNPKVPTRRVQYIRQNGSTAASTGRIQPY
  SKPTSWMTGPGLLSAQRVGPQSSDTAPFMVCTNPEGTHINTGAAGFGSGFDPPNGCLAPTNLEYKLQWYQ
  TPEGTGNNGNIIANPSLSMLRDQLLYKGNQTTYNLVGDIWMFPNQVWDRFPITRENPIWCKKPRADKHTI
  MDPFDGSIAMDHPPGTIFIKMAKIPVPTASNADSYLNIYCTGQVSCEIVWEVERYATKNWRPERRHTALG
  MSLGGESNYTPTYHVDPTGAYIQPTSYDQCMPVKTNINKVL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007455
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007455.1, YP_338088
• RefSeq ORF: 2013
• Locus ID: 3711587
• MW: 75.1 kDa