VP2 (NC_007455) Virus Tagged ORF Clone

CAT#: VC100560

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human bocavirus], codon optimized for human cell expression, YP_338089

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100560 represents NCBI reference of YP_338089 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGATACCGATATTCAAGACCAGCAGCCTGATACGGTCGACGCCCCACAGAATACCAGCGGAGGTG
  GGACAGGCTCCATTGGTGGTGGTAAAGGCTCCGGCGTGGGCATAAGTACAGGTGGATGGGTCGGGGGGTC
  TCATTTCTCTGACAAATACGTGGTCACAAAAAACACACGGCAATTTATCACCACTATACAAAATGGGCAT
  CTGTATAAAACAGAGGCCATCGAAACCACTAATCAGTCAGGCAAGAGCCAGCGATGCGTTACCACCCCTT
  GGACATATTTTAATTTCAATCAATACAGCTGCCACTTCTCCCCTCAAGACTGGCAGCGGCTGACAAATGA
  GTACAAGCGCTTTAGGCCAAAGGCAATGCAAGTGAAGATTTACAACCTGCAGATCAAACAGATCCTCTCC
  AACGGTGCAGACACCACCTACAATAACGACCTTACCGCCGGCGTACACATTTTCTGCGACGGTGAGCACG
  CCTACCCAAATGCCAGTCACCCCTGGGATGAGGACGTGATGCCGGACTTGCCATACAAAACTTGGAAACT
  CTTCCAGTATGGCTACATCCCCATTGAGAATGAACTCGCAGATCTTGACGGAAACGCGGCTGGAGGCAAT
  GCTACGGAGAAAGCTCTCTTGTATCAGATGCCATTCTTTCTGCTGGAGAATAGCGATCACCAGGTCCTTA
  GAACCGGTGAGAGTACAGAGTTTACATTCAACTTCGACTGCGAATGGGTGAACAATGAACGGGCCTATAT
  ACCACCCGGTCTGATGTTTAACCCAAAGGTTCCCACACGACGGGTCCAATATATTAGACAAAATGGCTCA
  ACTGCGGCTAGCACCGGTCGGATCCAGCCTTACAGTAAGCCTACTTCATGGATGACTGGTCCCGGGCTGC
  TTAGTGCCCAGCGGGTTGGCCCACAGTCATCTGACACTGCTCCTTTCATGGTGTGTACTAACCCTGAAGG
  CACACATATTAACACCGGCGCAGCCGGATTCGGCAGTGGCTTCGATCCTCCAAACGGATGCCTGGCCCCA
  ACCAACCTGGAGTATAAATTGCAGTGGTACCAGACACCTGAGGGCACCGGGAACAATGGCAACATCATCG
  CGAATCCTTCCTTGTCCATGCTGAGAGATCAGCTCCTGTACAAAGGTAACCAGACCACCTATAACTTGGT
  CGGAGACATATGGATGTTCCCAAACCAAGTCTGGGATCGGTTCCCTATCACGAGGGAAAACCCCATCTGG
  TGTAAAAAGCCCCGCGCCGACAAGCATACGATCATGGACCCCTTTGATGGCAGCATCGCGATGGACCACC
  CGCCTGGAACAATTTTCATCAAGATGGCCAAGATTCCAGTTCCCACTGCCTCTAATGCAGACTCCTACCT
  CAACATTTACTGTACCGGCCAAGTTAGTTGTGAAATCGTGTGGGAAGTGGAAAGGTATGCTACTAAGAAC
  TGGAGGCCTGAACGCCGACACACGGCCCTTGGCATGTCACTGGGCGGAGAATCTAATTATACTCCCACAT
  ATCATGTAGATCCTACCGGCGCTTATATCCAGCCAACAAGTTATGACCAATGTATGCCCGTTAAGACCAA
  CATCAACAAAGTCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100560 representing YP_338089
  Red=Cloning sites Green=Tags
  
  MSDTDIQDQQPDTVDAPQNTSGGGTGSIGGGKGSGVGISTGGWVGGSHFSDKYVVTKNTRQFITTIQNGH
  LYKTEAIETTNQSGKSQRCVTTPWTYFNFNQYSCHFSPQDWQRLTNEYKRFRPKAMQVKIYNLQIKQILS
  NGADTTYNNDLTAGVHIFCDGEHAYPNASHPWDEDVMPDLPYKTWKLFQYGYIPIENELADLDGNAAGGN
  ATEKALLYQMPFFLLENSDHQVLRTGESTEFTFNFDCEWVNNERAYIPPGLMFNPKVPTRRVQYIRQNGS
  TAASTGRIQPYSKPTSWMTGPGLLSAQRVGPQSSDTAPFMVCTNPEGTHINTGAAGFGSGFDPPNGCLAP
  TNLEYKLQWYQTPEGTGNNGNIIANPSLSMLRDQLLYKGNQTTYNLVGDIWMFPNQVWDRFPITRENPIW
  CKKPRADKHTIMDPFDGSIAMDHPPGTIFIKMAKIPVPTASNADSYLNIYCTGQVSCEIVWEVERYATKN
  WRPERRHTALGMSLGGESNYTPTYHVDPTGAYIQPTSYDQCMPVKTNINKVL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007455
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007455.1, YP_338089
• RefSeq ORF: 1626
• Locus ID: 3711588
• MW: 60.5 kDa