NP-1 (NC_007455) Virus Tagged ORF Clone

CAT#: VC100558

Myc-DDK-tagged ORF clone of viral ORF for NP-1 [Human bocavirus], codon optimized for human cell expression, YP_338087

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100558 represents NCBI reference of YP_338087 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCCGGAAACATGAAAGACAAACACAGAAGTTACAAGAGGAAGGGATCCCCCGAACGCGGCGAAA
  GAAAGAGGCACTGGCAAACAACACATCACCGAAGTCGAAGTCGGAGCCCTATTCGCCATTCCGGGGAACG
  GGGGTCCGGCTCATATCACCAAGAGCATCCTATCAGCCACCTGAGTTCCTGTACTGCATCTAAAACTTCA
  GACCAGGTGATGAAGACAAGGGAGTCCACCTCTGGCAAGAAAGACAATCGGACCAACCCCTACACAGTGT
  TCAGCCAGCATCGGGCAAGCAACCCCGAGGCTCCCGGCTGGTGTGGATTTTACTGGCACTCTACCAGAAT
  CGCCCGGGACGGGACCAATAGTATCTTTAATGAGATGAAACAGCAATTTCAGCAGCTCCAGATCGACAAC
  AAAATAGGATGGGACAATACCAGGGAACTGCTTTTTAACCAGAAAAAGACACTGGATCAAAAATATCGCA
  ACATGTTTTGGCATTTTAGGAACAATTCCGACTGCGAGAGATGCAATTATTGGGACGACGTCTACCGGAG
  GCATCTGGCTAACGTTTCATCCCAGACCGAAGCAGACGAGATCACCGATGAAGAGATGTTGAGTGCGGCT
  GAGAGTATGGAGGCGGATGCCTCAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100558 representing YP_338087
  Red=Cloning sites Green=Tags
  
  MSSGNMKDKHRSYKRKGSPERGERKRHWQTTHHRSRSRSPIRHSGERGSGSYHQEHPISHLSSCTASKTS
  DQVMKTRESTSGKKDNRTNPYTVFSQHRASNPEAPGWCGFYWHSTRIARDGTNSIFNEMKQQFQQLQIDN
  KIGWDNTRELLFNQKKTLDQKYRNMFWHFRNNSDCERCNYWDDVYRRHLANVSSQTEADEITDEEMLSAA
  ESMEADASN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007455
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007455.1, YP_338087
• RefSeq ORF: 657
• Locus ID: 3711586
• MW: 25.7 kDa