VP3 (NC_012800) Virus Tagged ORF Clone

CAT#: VC100610

Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human cosavirus A], codon optimized for human cell expression, YP_002956099

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100610 represents NCBI reference of YP_002956099 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCTATCCCAGTGCGACCCTTCCATAACTTCCAGCAGTTCAGCACCACAGTCCCCCTTCGAACCG
  AGCCCTGTTACGGGATGACTGTGACACCTCCTGTGGACTACATGCCTTTGCCAATCACTGACCTTGTTGA
  ACTCGCGAAAGTCCCAAGCTTTGTCACTGTAGCTAACAGTGACACCACAAGCGAGCGGTCATTTCCCTAC
  TTCAGCGTCAGTAACACAGAGCAAGGACGAAATCTTTTCAAAAGCTCAGTGGTCCTCAGCGACCTTCATT
  ATCAGCACACCCTGGTGGCCAATCTTGCCCGATACTTTTGTAATTATAGAGGGTCCCTGCAGTTCGACTT
  CATTGCCGCCACCACAGCTATGACAAGGGGTAAATTGCTTATCAGTTATACTCCCCCGGGGGCCGGCGAA
  CCACAATCAATTGACCAGGCCATGATGGGAACGTACGCTATCTGGGACCTCGGCTTGCAGAGCACCTTCA
  ATTTTGTCGTACCCTTTATCAGCGCAAGTGACTTCAGATTTAATACCTCTTCCGTGTCCAATGCATTGAA
  CAGCGATGGCTGGATAACAGTCTGGCTCATGAACCCTCTCACGTATCCACCAAGCACTCCACCAACGCAG
  CAAATTTTGATGCTTATGTCAGCAGGATCAGACTTCTCCTATAGGCTGCCTATCAGCCCCGGGTTTGCAG
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100610 representing YP_002956099
  Red=Cloning sites Green=Tags
  
  MGPIPVRPFHNFQQFSTTVPLRTEPCYGMTVTPPVDYMPLPITDLVELAKVPSFVTVANSDTTSERSFPY
  FSVSNTEQGRNLFKSSVVLSDLHYQHTLVANLARYFCNYRGSLQFDFIAATTAMTRGKLLISYTPPGAGE
  PQSIDQAMMGTYAIWDLGLQSTFNFVVPFISASDFRFNTSSVSNALNSDGWITVWLMNPLTYPPSTPPTQ
  QILMLMSAGSDFSYRLPISPGFAE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012800
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012800.1, YP_002956099
• RefSeq ORF: 702
• MW: 26.0 kDa