3A (NC_012800) Virus Tagged ORF Clone

CAT#: VC100615

Myc-DDK-tagged ORF clone of viral ORF for 3A [Human cosavirus A], codon optimized for human cell expression, YP_002956104

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100615 represents NCBI reference of YP_002956104 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCAACGATGATAGTCGGTTACACAAAGGATGATGACGGTATTGCAATTGTGGATTGCCTGGAGG
  AATGGAATAAGATTAAGGACAAGAAGAAAAAGCAGCTGGCGTTGGAAATGGTGGCTCAGGAGCTCAAGGA
  TAAGCACGAGGAGCATAAAGGGACCATCAAACTTCTTAAGATGTTTGTGACTGGACTGGGGGTGGTAGCT
  GCGGTGGCTGGAGCATATGCCACCATGAAGTACTTCACCAAGGATAAACCGAAAGAAGAGGAGGAAGAAC
  CAGAAGAAAAGAAGGAAAAGAAAACTGAGGAATCTAAGGAGGCGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100615 representing YP_002956104
  Red=Cloning sites Green=Tags
  
  MGPTMIVGYTKDDDGIAIVDCLEEWNKIKDKKKKQLALEMVAQELKDKHEEHKGTIKLLKMFVTGLGVVA
  AVAGAYATMKYFTKDKPKEEEEEPEEKKEKKTEESKEAA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012800
• ORF Size: 327 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012800.1, YP_002956104
• RefSeq ORF: 327
• MW: 12.3 kDa