2B (NC_012800) Virus Tagged ORF Clone

CAT#: VC100613

Myc-DDK-tagged ORF clone of viral ORF for 2B [Human cosavirus A], codon optimized for human cell expression, YP_002956102

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100613 represents NCBI reference of YP_002956102 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCATTTAACCCGGAATATACAGCTCACGGCCCCGTGACCGAGCTCATCCAGCTTGCCAGGAAGC
  CGGAAACTGTGGATAATGTTAATAGGCTCCTTACAACACTGAACACCCTTATGGCTAAGTGGAACAACCT
  TAAGGACACAGTAACCGACGCCGTGTTCCTCCGCGATATGGTTTGCCTTCTCGTCAAGCTCACATCCCTT
  ATGTACCTGGTGCACGGACAGGGACCCGGTGCTTACTTCGCAGCTGCTAGCATCCTGTTGGCCGATGGAA
  TTACATTTTTCGACTGGTACGAGAAGATCAAGATTTTCATGGCACGAAAGCTGCGCGTTAGCCCCCCATT
  TTTTCCCGCTGCTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100613 representing YP_002956102
  Red=Cloning sites Green=Tags
  
  MPAFNPEYTAHGPVTELIQLARKPETVDNVNRLLTTLNTLMAKWNNLKDTVTDAVFLRDMVCLLVKLTSL
  MYLVHGQGPGAYFAAASILLADGITFFDWYEKIKIFMARKLRVSPPFFPAAQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012800
• ORF Size: 366 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012800.1, YP_002956102
• RefSeq ORF: 366
• MW: 13.7 kDa