3C (NC_012800) Virus Tagged ORF Clone

CAT#: VC100614

Myc-DDK-tagged ORF clone of viral ORF for 3C [Human cosavirus A], codon optimized for human cell expression, YP_002956103

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100614 represents NCBI reference of YP_002956103 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCCGACCTGCGGGATTTCGTGACCTTCTTCAATGCTGCCCGGGGCGCCCAGTGGATGATCGACA
  GCCTTAAATCCCTCATAACTTGTATTAAACAATGGCTGGAGCTGGAGGAGGAGAATGAAGCCGTGCAACT
  GGAGAAGATGCTCATAGATTCTCCAAGACATTGTAAGGCGATTAATGACTATAATAGAGGCGATTCTTTT
  CAGAGGCCCACTAATTCCTTTGAGTTTATGGACCGACTGGTTGAATGTGCGACGAAGCTGGGCAAGGTAC
  AGATAGCCACTTATTTTAGAAATTTCACAACTGCCGACTCTGATACTTCCCGACCCGAACCTGTGGTCGT
  AGTGCTCCGGGGCAAGCCCGGTGTTGGGAAGAGTGCCGCCGCTACTGTTATGGCCGCCGCCGTTTCCAAA
  TTGCTCGTAGGATCCCAGAGCGTTTACACACTTAGCCCTGATACGGAGCATATGGACGGATATCATGGAC
  AGTTTGTGACCCTGATGGATGATCTCGGGCAGAATCCTGACGGCGAGGACTTCCGCTGTTTCTGTCAGAT
  GGTCTCATGCGCGCAGTACCGGCCCGCCATGGCCGACCTTAAGGATAAAGGCATCCTTTTTACCAGCAGA
  TTGCTTATCGCAACGACCAATCTTCCTGACTTCAATCCTGTCACAATCTCCGACCCCAGAGCTTTGGACC
  GCCGGATAACGTTCGATATTTTGGTGACTCCAGGCTCCGCAGCAACCAAGAACGGCAAGCTGGATCTGGC
  TGCGGCCCTGAAACCAGATGGTCCTGGAGAACATCCCTATACGAGCGACTGCCCTATACTTCACACTACA
  GGCCTGTTGCTCAAGAATTTGCGGAATAACCAGACAATGAATTTGAAAGACTTGGTTGATATGATCGTCA
  AGAGGATAAAGCACAAAAAAGAAGTGGGTAACATGCTTGACAGCCTGGTTGCACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100614 representing YP_002956103
  Red=Cloning sites Green=Tags
  
  MGPDLRDFVTFFNAARGAQWMIDSLKSLITCIKQWLELEEENEAVQLEKMLIDSPRHCKAINDYNRGDSF
  QRPTNSFEFMDRLVECATKLGKVQIATYFRNFTTADSDTSRPEPVVVVLRGKPGVGKSAAATVMAAAVSK
  LLVGSQSVYTLSPDTEHMDGYHGQFVTLMDDLGQNPDGEDFRCFCQMVSCAQYRPAMADLKDKGILFTSR
  LLIATTNLPDFNPVTISDPRALDRRITFDILVTPGSAATKNGKLDLAAALKPDGPGEHPYTSDCPILHTT
  GLLLKNLRNNQTMNLKDLVDMIVKRIKHKKEVGNMLDSLVAQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012800
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012800.1, YP_002956103
• RefSeq ORF: 966
• MW: 35.7 kDa