VP1 (NC_012800) Virus Tagged ORF Clone

CAT#: VC100611

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human cosavirus A], codon optimized for human cell expression, YP_002956100

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100611 represents NCBI reference of YP_002956100 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAGACCTCCGAACACCCAATGGATAACGCAGAATGCGGGAAGATCGACGACAAGGACGCAGGCA
  TGTTTAGCGGCCATTCCGTTGGACTGCCTACACCTCATACGTCCACCTCTTTTTTCTACGACAGATATCG
  CTTCGTAGGCATCGTAAAATCTGTCGTAAACAATACACCAAAACCCGTGAATATCTATGACGATACCGGC
  AAGGTGAAGAACCTTCAGCAGGTGTTTCCTACGTCTGATACACTCCTCCCACATAGTCTGATGTCACTGT
  CCCCCTGTGCCTCTGTGTGTGGGCAGCCCATCAGTTCATTTCTGTTTGCCCAGCGGGCCAACCCAAAGAA
  AACCCTGAAGCTGAGGTCCGGCGACGAATTTCTGTATCGCTGCTGTCCTTTCTCCTACATCAAATGCGAC
  CTGGAATTCACCGTGGTTCCACCCGCCAACAGTACCCGAGATTACATCGTGCATTGGTACCCACCAGGCG
  CCACGCTGGACGCTGGTGAAGTCGCTGTGGGAAACACATCTGGTTCAAATGGCTTCGATGATAATGGAAT
  GAACGCCGGTAGCTCCTTGTTTTCCTACAACCCAACCTTTCACGCTAGGGCACCCAGTAAGGTATCAGCT
  GTGATCCCATTCTGCCTGCCCGTTTCATTGCTCCCTCTTTATTTTGACGGATTTCCTGACTACAGTACGA
  CAAAGGGAATGTATGGCTGCTCACCGTCCTTTAGCTTCGGAACAATCTATATCGAGTCCGGCCTGCAAGA
  GACTTACTCTGTGTACATACGCTACAAGGATTTCAAGGGCTATGCCCCTCGGCCACTTATACGCACACCG
  CACATACGCCTTTCAGAACGGGCCAGGTATATCATGGCCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100611 representing YP_002956100
  Red=Cloning sites Green=Tags
  
  MGETSEHPMDNAECGKIDDKDAGMFSGHSVGLPTPHTSTSFFYDRYRFVGIVKSVVNNTPKPVNIYDDTG
  KVKNLQQVFPTSDTLLPHSLMSLSPCASVCGQPISSFLFAQRANPKKTLKLRSGDEFLYRCCPFSYIKCD
  LEFTVVPPANSTRDYIVHWYPPGATLDAGEVAVGNTSGSNGFDDNGMNAGSSLFSYNPTFHARAPSKVSA
  VIPFCLPVSLLPLYFDGFPDYSTTKGMYGCSPSFSFGTIYIESGLQETYSVYIRYKDFKGYAPRPLIRTP
  HIRLSERARYIMAD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012800
• ORF Size: 882 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012800.1, YP_002956100
• RefSeq ORF: 882
• MW: 32.5 kDa