VP2 (NC_012802) Virus Tagged ORF Clone

CAT#: VC100633

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human cosavirus D], codon optimized for human cell expression, YP_002956120

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100633 represents NCBI reference of YP_002956120 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCTAAGGTGGAAAACGACACGAATATGGAAGATAGGGTGATCACCTTGAAGGCCGGAAACACAA
  TAGTAAATTCACAAGCCAGTGAGGGTGTGCTCCATGGTTATGGCATCGGCACTAACACCCAAAGGCCAAG
  CTCTTGTGGGGACGATCCTTCAATCGCTACCCATTGTATCGAACGGGGTTTTACCATTAACCTGGCCGAC
  TGGGACAAGTCTAAAGAGAGCTGGCAGGCCCTGGTTTACAGGCTCTCCGATCATCTCAAGGATGATACAG
  TCGGAAACATGTTCTCAAAGACATTGGGAACCCACGCATACACGAAATGCGGATATAGAGTGAGTCTCCA
  GATCAATACCTCTCCATTTCACTCCGGACTGATCGGACTGTTCCTCGTTCCGGAATGCTGTATTCCCGCT
  AGCCTTAACATGGACTGGATCGACTTGAAGACCCAGCTGCCGCTGCTGACTAGTTCATCTCACTACCAGG
  GGCTGGGACTGTCCACTGGGCAGGGTACAATCTCTGAGAAAGGTTCAATCGATGCAGCGGGAACAATCCC
  GCAGCAGCTCTTCATTTACCCACACCAGCTGATCAATCCAAAGGATACCAATATTGCGAGTGTGGAAGTA
  CCTTATGTGAATTGTGCTCCGACATCTGACCCCATGATCCACAATATCTGGACTGCTTTGGTGGTGGTAA
  TTGCCCCGCTCCAATCCAACGCATCAGCTTCCCCAACCGTGGCTATGAGTATGACAGTGACTCCTGTTGG
  GGCTGTATTTAACGGCCTGAGGCATCCCGCTCCGAATGTACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100633 representing YP_002956120
  Red=Cloning sites Green=Tags
  
  MKPKVENDTNMEDRVITLKAGNTIVNSQASEGVLHGYGIGTNTQRPSSCGDDPSIATHCIERGFTINLAD
  WDKSKESWQALVYRLSDHLKDDTVGNMFSKTLGTHAYTKCGYRVSLQINTSPFHSGLIGLFLVPECCIPA
  SLNMDWIDLKTQLPLLTSSSHYQGLGLSTGQGTISEKGSIDAAGTIPQQLFIYPHQLINPKDTNIASVEV
  PYVNCAPTSDPMIHNIWTALVVVIAPLQSNASASPTVAMSMTVTPVGAVFNGLRHPAPNVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012802
• ORF Size: 813 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012802.1, YP_002956120
• RefSeq ORF: 813
• MW: 29.2 kDa