VP1 (NC_012802) Virus Tagged ORF Clone

CAT#: VC100635

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human cosavirus D], codon optimized for human cell expression, YP_002956122

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100635 represents NCBI reference of YP_002956122 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGATATCCACGATAATGCAGAGAAGGGCCTTACTGAAACAACTGATGCAACCCAGTTCTGTGGGG
  CCGCAGTGGGCTACACGACTAACCATTCCAACTGCGAATTCTTTTTTGATAGGTACCGATTCGTCGGCTT
  TATCGATGCTGTGAGGAATAATAAACGGAGCGTCATCAGCGTTTTCGATTCAAACAATAAGGTGAAACGG
  ATAGCCGAATTGTTCAAGGAACCAAATAAACCCGGTAACTACTTTACACTCTCTCCTAATCCAGCAATCA
  GTACAACTCCGATGTCCGCTTATATTGTGATTGACCATCCCTCCAGCACTAGCTCACCCTACACCCAGTA
  TGCAATCGCAACAACTGGCGATCCCTTTTTCCTCCGCTCATGTCCCTTCACCTACTTTCACTGCGACCTT
  GAAGTGACAATCAAACCAGAAAACGCAGTGAGTGGTGTCTGGAGGGCCACCTGGTATCCACCCGGATCCG
  ACTTGAAGGAGGACGAGGTAGTCCCCTCTTTCAGGACCACTGGAGAGAGCGGTAATATGTCTCTCACCGT
  AAACAACCGGGAACGGTCCACTATCTATAATACCTACCCTACATTCTATTCCCGAGATGGCCAATGTGTG
  TCCTTTAACATCCCCTACACCTCACCTTTGAGTGTGATTCCAACGCGCTTCGATGGATACCCCGATTACT
  CACGAACCGTTGGCGCTTATGGTGTTGCGCCTGCTAATCATTTTGGAACACTTACGGTGGCCGCTAACGA
  TGAGGGCTATAAGTTCTTTGTCTACGTGCGGTACAAAAACTTTAAGGGCTACGTCCCTAAGACACTCCCA
  CCCCAGCCTCTCTTCCTGAAGAACAGTAGGTCCCTCACGAACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100635 representing YP_002956122
  Red=Cloning sites Green=Tags
  
  MGDIHDNAEKGLTETTDATQFCGAAVGYTTNHSNCEFFFDRYRFVGFIDAVRNNKRSVISVFDSNNKVKR
  IAELFKEPNKPGNYFTLSPNPAISTTPMSAYIVIDHPSSTSSPYTQYAIATTGDPFFLRSCPFTYFHCDL
  EVTIKPENAVSGVWRATWYPPGSDLKEDEVVPSFRTTGESGNMSLTVNNRERSTIYNTYPTFYSRDGQCV
  SFNIPYTSPLSVIPTRFDGYPDYSRTVGAYGVAPANHFGTLTVAANDEGYKFFVYVRYKNFKGYVPKTLP
  PQPLFLKNSRSLTNE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012802
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012802.1, YP_002956122
• RefSeq ORF: 885
• MW: 33.1 kDa