3C (NC_012802) Virus Tagged ORF Clone

CAT#: VC100641

Myc-DDK-tagged ORF clone of viral ORF for 3C [Human cosavirus D], codon optimized for human cell expression, YP_002956128

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100641 represents NCBI reference of YP_002956128 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCCCTGCTGGACCTCGAAAAGAAAGTCCTGGCCAACGTCCAGCCCTTTATCCTCCGAGTGGCAG
  GACGCGACTACATCCAGTCCTGCCTGTTTGTGGGTAAAAGAGTCTTCCTGGTGAACAAACACGCAATTGA
  CTCTGTCGAGCAGAAATTCCAGGTCGCTGGCAAGACATACGACTTGGATGACGTTGACGTCGCTATTCTG
  GATACCGAGTACGGACTCACAGACGTGGCCGCCGTAAAGCTGAACACCGGCCCTGAGTGGAAGAATCTGA
  GTAAGCTCTTTGTTTCTCTGGACACCACCTTGCACCCCGGCACACGGATTACTATCCTTTCCAATGACCA
  ACTGAACATGGTGCGAGAGGGCAGTTTTCTCCGGAACGAGGACGATATCCCAACCAATATAGGCCCTATT
  CCCTTCGTTATGCTGTATAAAGCCTCATCTTATTTCGGTATGTGCGGTTCAGCAGTAGTAACGCGCATAG
  GAGATTGCCCCGGTATACTGGGACTGCACTGTGCAGGCGGCGGAGGCGTATGCGTCGCCTCACGGGTTAC
  AAAAAGAATGGTTGAGACAGTCCTGAAGTATTTTTACCCCCCTCAGGTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100641 representing YP_002956128
  Red=Cloning sites Green=Tags
  
  MGPLLDLEKKVLANVQPFILRVAGRDYIQSCLFVGKRVFLVNKHAIDSVEQKFQVAGKTYDLDDVDVAIL
  DTEYGLTDVAAVKLNTGPEWKNLSKLFVSLDTTLHPGTRITILSNDQLNMVREGSFLRNEDDIPTNIGPI
  PFVMLYKASSYFGMCGSAVVTRIGDCPGILGLHCAGGGGVCVASRVTKRMVETVLKYFYPPQVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012802
• ORF Size: 612 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012802.1, YP_002956128
• RefSeq ORF: 612
• MW: 22.4 kDa