VP3 (NC_012802) Virus Tagged ORF Clone

CAT#: VC100634

Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human cosavirus D], codon optimized for human cell expression, YP_002956121

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100634 represents NCBI reference of YP_002956121 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGCTATCCCCGTTCGCATGACCCAAAATAGCGGCCAATTCTCAACCACCCTCCCCGCGCGAATGG
  AACCTTGCTACGGCCTTACTCCCAACCCCACCCGAGACTTTCTCCCTCCAGTCGTTGAGGATCTGCTCAG
  TATCGCCAAGGTGCCCTGCTTTTTGCTCGCCGATGAGGACACTACTAAACAGAAACCCTACTTTCTGATA
  TCTAATGCGTCCAGCACTCAGACGGCAGTGTTTGAAATGAACGTGATACTCAGCGAATACGCTCTGCAGA
  GAACTTTCGTGAGTATGTTTGGCAAGTTCTTCTGTAACTACCGGGGCTCAATCCAAATTACTGCATGGGC
  GGCCGTGACCGCCATGACCCGGGGAAAGCTCCTGTTCAGTTACACACCACCTGGGGCTGGGAAGCCCCAA
  AATATCAAGCAGGCTATGATGGGGACTTATACTATCTGGGACCTGGGGCTGCAGTCCACCCTCAACTTTA
  CAATCCCATACATCTCAAGCGTTGACTTCAGAATCAACTCCAGGGCTGTGGCTAGTGCCCTGAATGCTGA
  CGGCTGGCTGACCATCTGGATCCTCAATCCAATAACATATCCGCCGCAAACTCCACCCCAGCAAGCTATC
  CTGCTGATGGCGTCCGCTGGCTCTGACTTCAGCTACCGGCTGCCTATTAATCCGCCTTTTGTACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100634 representing YP_002956121
  Red=Cloning sites Green=Tags
  
  MTAIPVRMTQNSGQFSTTLPARMEPCYGLTPNPTRDFLPPVVEDLLSIAKVPCFLLADEDTTKQKPYFLI
  SNASSTQTAVFEMNVILSEYALQRTFVSMFGKFFCNYRGSIQITAWAAVTAMTRGKLLFSYTPPGAGKPQ
  NIKQAMMGTYTIWDLGLQSTLNFTIPYISSVDFRINSRAVASALNADGWLTIWILNPITYPPQTPPQQAI
  LLMASAGSDFSYRLPINPPFVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012802
• ORF Size: 696 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012802.1, YP_002956121
• RefSeq ORF: 696
• MW: 25.7 kDa