3C (NC_012802) Virus Tagged ORF Clone

CAT#: VC100638

Myc-DDK-tagged ORF clone of viral ORF for 3C [Human cosavirus D], codon optimized for human cell expression, YP_002956125

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100638 represents NCBI reference of YP_002956125 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCTGATTTGAGAGATCTGGTGACCTTCTTTAATGCCGCCCGAGGGGCACAGTGGATGGTTGACT
  CCATAAGAGGCCTGATCTCTTGGATCAAGCAGTGGCTGGAGCTGGAAGAGGCTAACGAGGCGGTCCAGTT
  CGAACGGCTCTTGATCGAATCACCCAAGCACTGTAAAGCTATTAACGACTATAACGTTGGGAAATCATTC
  ATTAGGCCCGAGAATTCATTTGATTTTATGGAGAAGCTGGTCGACTCAGCCACCAAGCTGGGGAAGGTGA
  ATATTGCAGGATACTTTCGGTCTTTTACCTCCGTCGATACAGATACCGCTCGGATGGAGCCAGTCGTTCT
  GGTTTTGCGCGGAAAACCCGGAGCCGGGAAGAGTGCCGCCGCTACTATTATCACAGCCGCCGTAAGTAAA
  ATCTTGACCGGCACACAAAGCGTGTACTCCCTGTCACCCGATACCGAACATATGGATGGATACCACGGTC
  AGTTTGCCATGATCATGGATGACCTGGGTCAGAATCCCGATGGGGAGGATTTTCGGACGTTTTGCCAGAT
  GATCTCCGTCGCTCAGTATAGACCCTCAATGGCCGATCTGAAGGATAAGGGTATCCTGTTTAAATCCCAA
  TTTATCGTGGCCACTACAAACCTGCCGGAGTTCAGGCCACTGACCGTGAGTGACCGAGGGGCCGTCGACC
  GCCGAATCACATTTGATATCGGAGTCACCCCAGGAACTGCGGTTACAAAAAACGGTAAGCTGGATCTGGC
  CATGGCTCTTAAACCCGACGGGGAAGGAGAGTTCCCCTACTCCTGCGATTGTCAGATCTTGCACACAACG
  GGGCTGGCCCTTCAAAACCTGCGCACCGGCAAAACCATGAATATCAAAGAGCTTGTGGACCTCATTGTCA
  AAAAGATCAAATCTAAAAGGACGACATCTGGGATGCTGGAGGGTTTGGTGGTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100638 representing YP_002956125
  Red=Cloning sites Green=Tags
  
  MGPDLRDLVTFFNAARGAQWMVDSIRGLISWIKQWLELEEANEAVQFERLLIESPKHCKAINDYNVGKSF
  IRPENSFDFMEKLVDSATKLGKVNIAGYFRSFTSVDTDTARMEPVVLVLRGKPGAGKSAAATIITAAVSK
  ILTGTQSVYSLSPDTEHMDGYHGQFAMIMDDLGQNPDGEDFRTFCQMISVAQYRPSMADLKDKGILFKSQ
  FIVATTNLPEFRPLTVSDRGAVDRRITFDIGVTPGTAVTKNGKLDLAMALKPDGEGEFPYSCDCQILHTT
  GLALQNLRTGKTMNIKELVDLIVKKIKSKRTTSGMLEGLVVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012802
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012802.1, YP_002956125
• RefSeq ORF: 966
• MW: 35.6 kDa