VP2 (NC_013115) Virus Tagged ORF Clone

CAT#: VC100681

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human enterovirus 107], codon optimized for human cell expression, YP_003104786

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100681 represents NCBI reference of YP_003104786 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCTACTGTGGAAGAGTGTGGCTTCTCTGACCGGGTCAGGTCTATAACACTGGGTAACAGCACCA
  TCACTACCCAGGAGTGCGCTAACGTAGTGGTGGGATATGGTGTGTGGCCTGAATATCTTTCCGATAAGGA
  GGCCACTGCTGAGGATCAGCCTACTCAGCCAGACGTGGCAACCTGCAGATTTTATACTCTGGACAGCGTT
  CAATGGGAGAAGAACTCTGCTGGTTGGTGGTGGAAGTTCCCCGAGGCACTGAGCGACATGGGGCTCTTTG
  GTCAGAACATGATGTACCATTATCTGGGAAGAGCGGGATACACAATTCATGTCCAATGCAACGCGAGCAA
  GTTTCACCAGGGCTGCCTGTTGGTAGTGTGCGTACCTGAGGCGGAGATGGGAGGGGCTAAAGTGGGCCAG
  GCATTCGAGGCCGACGCCCTTGCAAAGGGTGACAGGGCCATGCTGTTTACCGATCAAAAGCAGACTGACA
  AGACAAAGATCCAATCAGTGGTGCACAACGCCGGGATGGGAGTGGGCGTGGGAAATCTGACAATCTTCCC
  GCACCAGTGGATTAACCTCCGCACTAACAACTCTGCGACGATAGTGATGCCGTATATCAACTCAGTGCCG
  ATGGATAACATGTTCCGGCACTACAATTTTACTCTGATGATTATCCCCTTCGTGAAACTGGACTACACCG
  AAGCTACCAGTTACGTTCCGATCACCGTTACGGTCGCACCCATGTGCGCTGAGTACAATGGACTGAGACT
  GGGGCAGGTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100681 representing YP_003104786
  Red=Cloning sites Green=Tags
  
  MSPTVEECGFSDRVRSITLGNSTITTQECANVVVGYGVWPEYLSDKEATAEDQPTQPDVATCRFYTLDSV
  QWEKNSAGWWWKFPEALSDMGLFGQNMMYHYLGRAGYTIHVQCNASKFHQGCLLVVCVPEAEMGGAKVGQ
  AFEADALAKGDRAMLFTDQKQTDKTKIQSVVHNAGMGVGVGNLTIFPHQWINLRTNNSATIVMPYINSVP
  MDNMFRHYNFTLMIIPFVKLDYTEATSYVPITVTVAPMCAEYNGLRLGQVQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013115
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013115.1, YP_003104786
• RefSeq ORF: 783
• MW: 29.1 kDa