2A (NC_013115) Virus Tagged ORF Clone

CAT#: VC100684

Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus 107], codon optimized for human cell expression, YP_003104789

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100684 represents NCBI reference of YP_003104789 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCTTTCGGTCAGCAGAGTGGGGCCGTGTACGTTGGCAATTATCGCGTCGTTAACAGGCACCTCG
  CCACTCACAGTGACTGGCAGAACTGCGTGTGGGAAGATTCCAATAGAGATCTGCTCGTCTCAACCACGAC
  AGCCCACGGATGTGATACCATCGCTAGATGTCAATGTACGACCGGGGTGTACTTCTGCGCTTCCAAGAAC
  AAGCACTACCCAGTCGTTTTTGAAGGTCCCGGACTGGTCGAGGTGCAGGAAAGCGAATATTACCCCCGGA
  GATATCAATCCCATGTATTGTTGGCCGCAGGGTTCTCAGAGCCCGGTGACTGCGGAGGCATTTTGAGGTG
  TGAGCATGGCGTCATAGGGATTGTAACGATGGGAGGCGAAGGCGTTGTTGGGTTTGCTGATGTTAGGGAC
  CTGCTCTGGCTCGAAGATGATGCCATGGAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100684 representing YP_003104789
  Red=Cloning sites Green=Tags
  
  MGPFGQQSGAVYVGNYRVVNRHLATHSDWQNCVWEDSNRDLLVSTTTAHGCDTIARCQCTTGVYFCASKN
  KHYPVVFEGPGLVEVQESEYYPRRYQSHVLLAAGFSEPGDCGGILRCEHGVIGIVTMGGEGVVGFADVRD
  LLWLEDDAMEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013115
• ORF Size: 453 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013115.1, YP_003104789
• RefSeq ORF: 453
• MW: 16.6 kDa