VP3 (NC_013115) Virus Tagged ORF Clone

CAT#: VC100682

Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human enterovirus 107], codon optimized for human cell expression, YP_003104787

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100682 represents NCBI reference of YP_003104787 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCTGCCTACCCTCAATACCCCGGGTTCTAGTCAATTTTTGACCAGTGACGATTTCCAATCCCCAT
  GCGCCCTGCCGCAATTTGACGTCACCCCACCCATGAAGATTCCTGGGGAAGTGCATAACCTGATGGAGAT
  TGCCGAAGTGGACTCCGTAGTGCCTGTAAACAATGTCAACGGAACCACTGACCAAATGGACATGTTTCGC
  ATACCCGTCACGATTGGGAACAAGACCCAGGACCAGGTGTTCGGAGTTAGATTGCAGCCAGGGCTCGACT
  CAGTGTTTAAGCACACGCTGCTGGGTGAGATCCTCAATTACTACGCACACTGGTCCGGAAGTATTAAACT
  TACCTTTATGTTTTGTGGTAGTGCTATGGCCACTGGTAAGTTCTTGATCGCGTACAGTCCTCCGGGAGCA
  GATCCACCCACCAACCGAAAAGGAGCAATGCTCGGCAAGAATATTGTGTGGGACATTGGTCTTCAGTCCA
  GTTGCGTGCTCTGTATGCCATGGATCTCACAGACTCACTATCGACTTGTTCAGCAAGACGAATATACGAG
  CGCAGGCTTCATCACTGGATGGTACCAAACTGGCGTGGTGATCCCGCCTGGAACCCCCTCAACCTGCTCA
  ATCCTCTGCTTCGTGTCCGCATGTAATGATTTTTCCGTGCGCATGCTGCGGGACACACCCTTTACTAGCC
  AGACCGCAATTCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100682 representing YP_003104787
  Red=Cloning sites Green=Tags
  
  MGLPTLNTPGSSQFLTSDDFQSPCALPQFDVTPPMKIPGEVHNLMEIAEVDSVVPVNNVNGTTDQMDMFR
  IPVTIGNKTQDQVFGVRLQPGLDSVFKHTLLGEILNYYAHWSGSIKLTFMFCGSAMATGKFLIAYSPPGA
  DPPTNRKGAMLGKNIVWDIGLQSSCVLCMPWISQTHYRLVQQDEYTSAGFITGWYQTGVVIPPGTPSTCS
  ILCFVSACNDFSVRMLRDTPFTSQTAILQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013115
• ORF Size: 717 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013115.1, YP_003104787
• RefSeq ORF: 717
• MW: 26.2 kDa