2C (NC_013115) Virus Tagged ORF Clone

CAT#: VC100686

Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus 107], codon optimized for human cell expression, YP_003104791

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100686 represents NCBI reference of YP_003104791 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAACAACTGGCTCAAGAAATTCACGGAGATGACTAACGCTTGTAAAGGGATGGAATGGATTGCTG
  TGAAAATACAGAAGTTCATAGAGTGGCTGAAAGTAAAAATCCTGCCTGAGGTGAGAGAAAAACATGAGTT
  CCTGAACCGCCTTAAGCAGCTCCCCCTGCTGGAAAGCCAGATCGCGACTATCGAGCAGTCAGCACCCTCC
  CAGTCAGATCAAGAGCAGCTGTTTTCTAATGTCCAATATTTCGCACACTATTGCCGCAAGTACGCACCTT
  TGTATGCCGCCGAGGCAAAAAGAGTGTTCTCTCTTGAGAAAAAGATGAGCAACTATATTCAATTTAAAAG
  TAAATGCCGCATTGAGCCTGTATGCTTGCTCCTGCATGGATCTCCAGGCGCCGGGAAAAGCGTCGCGACC
  AACCTTATAGGCCGCAGCCTCGCAGAAAAGCTGAACAGTAGTGTTTATTCTTTGCCTCCCGATCCCGACC
  ACTTTGACGGATACAAACAGCAGGCCGTTGTAATTATGGACGACCTCTGTCAGAACCCCGATGGGAAGGA
  CGTGTCTCTCTTCTGTCAGATGGTGAGCTCTGTTGACTTCGTGCCGCCAATGGCAGCGCTGGAAGAGAAA
  GGCATTCTCTTCACATCACCATTCGTCCTCGCTTCTACGAACGCCGGTTCTATTAACGCTCCAACGGTGT
  CTGATTCAAGGGCACTTGCCCGGAGGTTCCACTTCGACATGAACATCGAAGTCATCTCTATGTACAGCCA
  GAATGGAAAAATCAACATGCCAATGAGCGTGAAGACGTGCGATGAGGAATGCTGTCCCGTCAATTTCAAA
  AGGTGCTGCCCTCTTGTGTGTGGCAAGGCCATACAATTCATAGATAGGCGGACCCAGGTGCGGTATTCCC
  TCGATATGTTGGTGACGGAAATGTTTCGGGAATACAACCACAGACACTCTGTAGGTGCTACTCTGGAGGC
  TTTGTTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100686 representing YP_003104791
  Red=Cloning sites Green=Tags
  
  MNNNWLKKFTEMTNACKGMEWIAVKIQKFIEWLKVKILPEVREKHEFLNRLKQLPLLESQIATIEQSAPS
  QSDQEQLFSNVQYFAHYCRKYAPLYAAEAKRVFSLEKKMSNYIQFKSKCRIEPVCLLLHGSPGAGKSVAT
  NLIGRSLAEKLNSSVYSLPPDPDHFDGYKQQAVVIMDDLCQNPDGKDVSLFCQMVSSVDFVPPMAALEEK
  GILFTSPFVLASTNAGSINAPTVSDSRALARRFHFDMNIEVISMYSQNGKINMPMSVKTCDEECCPVNFK
  RCCPLVCGKAIQFIDRRTQVRYSLDMLVTEMFREYNHRHSVGATLEALFQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013115
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013115.1, YP_003104791
• RefSeq ORF: 990
• MW: 37.5 kDa