VP2 protein (NC_001428) Virus Tagged ORF Clone

CAT#: VC100741

Myc-DDK-tagged ORF clone of viral ORF for VP2 protein [Human enterovirus C], codon optimized for human cell expression, NP_740584

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100741 represents NCBI reference of NP_740584 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCTAACGTCGAAGCCTGTGGATACTCCGATCGAGTCAGACAGATTACACTGGGAAATAGCACCA
  TCACAACCCAAGAAGCAGCTAACGCTATTGTGGCATACGGCGAATGGCCCACTTACATCAACGACAGTGA
  AGCAAATCCTGTGGATGCTCCTACTGAACCCGACGTTTCCTCCAACAGATTCTACACACTGGAAAGTGTC
  AGCTGGAAAACCACGAGCAGGGGCTGGTGGTGGAAGCTTCCGGACTGTTTGAAAGACATGGGTATGTTCG
  GGCAGAATATGTATTATCATTACTTGGGTAGATCCGGCTATACTATACACGTGCAGTGTAACGCCAGTAA
  ATTCCATCAGGGAGCACTCGGTGTGTTCCTGATCCCAGAGTTTGTGATGGCCTGCAACACAGAGAGCAAG
  ACTAGCTACGTGTCTTATATCAACGCAAACCCAGGGGAACGCGGCGGGGAATTCACCAACACGTACAATC
  CTAGCAATACAGATGTATCAGAAGGCAGGCAGTTCGCCGCCCTGGATTATCTGCTTGGCTCTGGTGTGCT
  TGCTGGTAATGCTTTTGTGTATCCACACCAAATCATTAACCTTCGGACCAACAATTCTGCTACTATCGTG
  GTACCCTACGTTAACTCACTGGTTATCGACTGCATGGCAAAACACAACAACTGGGGAATAGTGATACTGC
  CGTTGGCCCCACTGGCCTTTGCCGCCACGAGTAGTCCGCAGGTTCCTATCACCGTCACCATAGCTCCCAT
  GTGCACCGAGTTCAACGGCCTGCGGAACATTACCATCCCTGTTCATCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100741 representing NP_740584
  Red=Cloning sites Green=Tags
  
  MSPNVEACGYSDRVRQITLGNSTITTQEAANAIVAYGEWPTYINDSEANPVDAPTEPDVSSNRFYTLESV
  SWKTTSRGWWWKLPDCLKDMGMFGQNMYYHYLGRSGYTIHVQCNASKFHQGALGVFLIPEFVMACNTESK
  TSYVSYINANPGERGGEFTNTYNPSNTDVSEGRQFAALDYLLGSGVLAGNAFVYPHQIINLRTNNSATIV
  VPYVNSLVIDCMAKHNNWGIVILPLAPLAFAATSSPQVPITVTIAPMCTEFNGLRNITIPVHQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001428
• ORF Size: 819 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001428.1, NP_740584
• RefSeq ORF: 819
• MW: 30.1 kDa